Vega3 sb easy probe

Manufactured by TESCAN

Sourced in Czechia

The Vega3 SB Easy Probe is a scanning electron microscope (SEM) designed for high-resolution imaging and analysis. It features a compact and user-friendly design, enabling easy operation and sample handling. The Vega3 SB Easy Probe provides stable and reliable performance for a variety of applications.

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Lab products found in correlation

Nis element, by Nikon (2 mentions) Nova nanosem 230, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

VEGA3 (428 citations) VEGA3 SB (10 citations)

6 protocols using vega3 sb easy probe

SEM Analysis of Fibrous Mats

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The samples were sputter-coated with a 7 nm layer of
gold and imaged by means of a TESCAN Vega 3SB Easy Probe (TESCAN s.r.o.,
Czech Republic) scanning electron microscope. Images of both the inner
(in contact with the underlying spunbond layer) and outer sides of
the fibrous mats prior to and following sterilization were taken in
three independent positions. The fibers (500 nos.) were assessed by
means of NIS Elements software (LIM s.r.o., Czech Republic) for fiber
diameter measurement purposes. The data is shown in box plot form
with the representation of the upper and lower quartiles and the median
as boxes and the variability showing the maximum and minimum measured
values.

Horakova J., Klicova M., Erben J., Klapstova A., Novotny V., Behalek L, & Chvojka J. (2020). Impact of Various Sterilization and Disinfection Techniques on Electrospun Poly-ε-caprolactone. ACS Omega, 5(15), 8885-8892.

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Characterization of Functionalized Polyacrylonitrile Nanofibers

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All non-functionalized PAs and PAs functionalized with AgNO₃ were characterized in terms of surface density, thickness, air permeability, and fiber diameter in the study of Lencova et al. [37 (link)]. The same parameters for CHX PAs were characterized in this study. Briefly, the surface density was evaluated from 10 × 10 cm samples taken from at least five different parts of the material; the thickness was measured with a Corp ID-C112XB device (Mitutoyo, Teplice, Czech Republic); air permeability was measured using a TEXTEST FX 3300 device (TexTest Instruments, Schwerzenbach, Switzerland). To evaluate fiber diameters, CHX PAs were sputter-coated with gold (14 nm), and pictures were taken using a scanning electron microscope (SEM) Tescan Vega3 SB Easy Probe (TESCAN, Brno, Czech Republic), and Nova NanoSEM 230 (Thermo Fisher Scientific, Waltham, MA, USA), respectively. The fiber diameter was evaluated from five pictures of each sample, at least 100 measurements from one sample in total, using the software NIS Elements (Nikon, Tokyo, Japan). Pore size of all 13 tested PAs was measured in this study; pore size was measured in a CFP-1200 AEL Capillary Flow Porometer (Porous Materials Inc., Ithaca, NY, USA) using the bubble point method.

Lencova S., Zdenkova K., Jencova V., Demnerova K., Zemanova K., Kolackova R., Hozdova K, & Stiborova H. (2021). Benefits of Polyamide Nanofibrous Materials: Antibacterial Activity and Retention Ability for Staphylococcus Aureus. Nanomaterials, 11(2), 480.

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Nanofibrous Materials Characterization by SEM

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Scanning
electron microscopy (SEM) was used for the morphological analysis
of nanofibrous materials. Electrospun samples (5 × 5 mm²) were sputtered with a thin layer of gold and imaged by a Vega 3SB
Easy Probe (TESCAN, Czechia) scanning electron microscope. The fiber
diameters (100 measurements for each sample) were measured by using
FIJI/ImageJ software (NIH). Further, a quantified estimation of characteristic
distances between fibers forming “eyes” in SEM photographs
of fiber systems was provided according to Lurie et al.^{9 (link)} We estimate here the characteristic intrafiber
distance δ according to eq 1. Detailed information is provided
in the Supporting Information. Next, SEM
at low voltage to analyze the fiber surface was performed. The samples
were sputtered with 1 nm of platinum for surface analysis. High-resolution
imaging was performed on the FE-SEM Zeiss Ultra Plus equipped with
the energy-dispersive spectroscopy (Oxford X-Max20, Carl Zeiss, Germany)
using gentle beam conditions of 1 kV/1.6 pA.

Lencova S., Stindlova M., Havlickova K., Jencova V., Peroutka V., Navratilova K., Zdenkova K., Stiborova H., Hauzerova S., Kostakova E.K., Jankovsky O., Kejzlar P., Lukas D, & Demnerova K. (2024). Influence of Fiber Diameter of Polycaprolactone Nanofibrous Materials on Biofilm Formation and Retention of Bacterial Cells. ACS Applied Materials & Interfaces, 16(20), 25813-25824.

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SEM Analysis of PCL Nanomaterial Biofilms

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Samples of PCL nanomaterials,
after both filtration and biofilm formation, were analyzed by SEM.
The PCLs with the retained cells or matured biofilm were gently rinsed
with phosphate-buffered saline (PBS) and fixed (4 °C, 15 min)
in frozen ethanol (99.8%, Penta, Czechia). The samples were dewatered
with ethanol (concentrations 60.0–99.9%; 5 min per each concentration,
room temperature). After drying (room temperature, at least 24 h),
the samples were sputter-coated with gold (14 nm) and observed using
a SEM Tescan Vega3 SB Easy Probe (Tescan, Czechia).

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Electrospun Fiber Diameter Characterization

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Electrospun samples with dimensions of 0.5 × 0.5 cm were sputtered with a thin layer of gold and imaged using a Vega 3SB Easy Probe (TESCAN, Brno, Czech Republic) scanning electron microscope. The fiber diameters (approximately 100 measurements for each sample) were measured using FIJI/ImageJ software (version number 2.1.0/1.53t, NIH).

Havlickova K., Kuzelova Kostakova E., Lisnenko M., Hauzerova S., Stuchlik M., Vrchovecka S., Vistejnova L., Molacek J., Lukas D., Prochazkova R., Horakova J., Jakubkova S., Heczkova B, & Jencova V. (2024). The Impacts of the Sterilization Method and the Electrospinning Conditions of Nanofibrous Biodegradable Layers on Their Degradation and Hemocompatibility Behavior. Polymers, 16(8), 1029.

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Characterizing PA Nanofibrous Materials

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The prepared PA nanofibrous materials were characterized in terms of their morphology. Scanning electron microscopy (SEM) using a Tescan Vega3 SB Easy Probe (TESCAN, Czechia) was used to assess PA homogeneity and to investigate the possible effect of the functionalization on PA morphology. Before SEM analysis, the PA nanofibers were sputter-coated with gold (14 nm). The software NIS Elements (Nikon, Japan) was used to perform the morphological analysis. Fiber diameter, surface density, and air permeability of the PAs were determined. Fiber diameter was evaluated from 100 measurements of different areas (Lencova et al., 2021a (link)), surface density (the thickness of PAs) was measured using a Corp ID-C112XB (Mitutoyo, Czechia), and air permeability was measured with a TEXTEST FX 3300 (TexTest Instruments, Switzerland). The air permeability measurement was done with standardized pressure gradient at room temperature; was based on measuring the amount of air that passed between the opposite sides of the PA, relative to the surface area and time. The air flow pressure was 200 Pa.

Lencova S., Stiborova H., Munzarova M., Demnerova K, & Zdenkova K. (2022). Potential of Polyamide Nanofibers With Natamycin, Rosemary Extract, and Green Tea Extract in Active Food Packaging Development: Interactions With Food Pathogens and Assessment of Microbial Risks Elimination. Frontiers in Microbiology, 13, 857423.

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