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Mithras lb940 elisa plate reader

Manufactured by Berthold Technologies

The Mithras LB940 is an ELISA plate reader designed for automated measurement of absorbance in microplates. It is capable of conducting photometric measurements to quantify the concentration of target analytes in samples.

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2 protocols using mithras lb940 elisa plate reader

1

Serum HMGB1 protein quantification

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Serum samples were obtained from NCD and SSD-fed mice and concentrations of HMGB1 protein were determined by commercial HMGB1 ELISA colorimetric kit (Novus Biologicals) according to the manufacturer’s protocol. Plates were analyzed using a Mithras LB940 ELISA plate reader (Berthold technologies).
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2

Serum Insulin and IFN-gamma Analysis

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To analyze serum insulin concentrations, animals were fasted overnight and injected with 1 g/kg of D-glucose. Blood was isolated from the v. saphena using heparin coated plastics. Insulin was measured in plasma using the mouse ultrasensitive insulin ELISA kit (Alpco). IFN-g concentrations were measured with the mouse IFN-gamma Platinum ELISA (eBioscience) according to manufacturer's protocol. Plates were analyzed using a Mithras LB940 ELISA plate reader (Berthold technologies).
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