Materials used for the pellet formulation were acyclovir of European Pharmacopea quality, which was obtained from Union Quimico Farmaceutica S.A., Barcelona, Spain. Chitosan (medium molecular weight 90–310 kDa, degree of deacetylation 82%) and sodium alginate (molecular weight 120,000–190,000 g/mol) were purchased from Sigma-Aldrich Chemie GmbH, Steinheim, Germany, Methocel K100M; co-processed microcrystalline cellulose with lactose monohydrate and natrium carboxymethyl cellulose (Specicell®140) were supplied by The Dow Chemical company, Michigan, USA. Acetic acid, sodium chloride, and hydrochloric acid were purchased from Centralchem s.r.o, Bratislava, Slovakia. Pellets were filled into hard gelatin capsules, which were provided by Interpharm a.s., Bratislava, Slovakia. Purified water was freshly prepared by distillation.
Sodium chloride (nacl)
Manufactured by Centralchem
Sourced in Slovakia
Sodium chloride is a chemical compound with the formula NaCl. It is a white crystalline solid that is the main source of sodium and chloride ions in the body. Sodium chloride is commonly used in various laboratory applications as a reference material or for the preparation of buffer solutions.
Automatically generated - may contain errors
Lab products found in correlation
Acetic acid, by Centralchem (1 mentions)
Hydrochloric acid (hcl), by Centralchem (1 mentions)
Sodium alginate, by Merck Group (1 mentions)
Chitosan, by Merck Group (1 mentions)
Isopropyl myristate, by Merck Group (1 mentions)
Carrageenan, by Merck Group (1 mentions)
Isopropyl alcohol, by Centralchem (1 mentions)
Polysorbate 80, by Centralchem (1 mentions)
Alginic acid sodium salt, by Merck Group (1 mentions)
Milli q system, by Merck Group (1 mentions)
Chemidoc mp, by Bio-Rad (1 mentions)
4 protocols using sodium chloride (nacl)
1
Acyclovir Chitosan-Alginate Pellet Formulation
2
Topical Minoxidil Formulation Development
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All materials were of analytical grade and used as received without further purification. Alginic acid sodium salt, carrageenan and isopropyl myristate, minoxidil (MXD) were purchased from Sigma Aldrich (Steinheim, Germany). Polysorbate 80, sodium chloride, ethanol (96%) and isopropyl alcohol were purchased from Centralchem (Bratislava, Slovakia). Propylene glycol (PG) was purchased from Interpharm (Bratislava, Slovakia), Carbopol 71G NF from Lubrizol (Cleveland, USA), soybean lecithin from Dimica (ASP, Divina, Slovakia) and gelatin from Valúch (Banská Bystica, Slovakia). Double-distilled water used for the experiments was prepared at the Department of Galenic Pharmacy (Kavalier, Labo SK, Bratislava, Slovakia). Porcine ear skin was acquired from a local slaughterhouse (Stará Huta, Slovakia).
3
Synthesis and Characterization of Gold Nanoparticles
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Gold nanoparticles were synthesized at the Institute of Analytical Chemistry, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava (Slovakia). Low-molecular-weight salmon sperm dsDNA was purchased from Sigma-Aldrich (Germany). Na2HPO4, NaH2PO4, K3[Fe(CN)6], and K4[Fe(CN)6]·3 H2O were obtained from Lachema Brno (Czech Republic). CH3CH2OH and NaCl were provided by Centralchem Bratislava (Slovakia). Other chemicals were obtained from Mikrochem (Slovakia) and Lachema (Czech Republic). All chemicals were of analytical reagent-grade purity. Nanopure water with resistivity above 18 MΩ·cm (Millipore Milli-Q system) was used for all experiments.
4
Quantifying RdRp and ASC1R Interaction
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ASC1R and the 119 nt target RdRp sequence were mixed in an equimolar ratio (1012 molecules each) and incubated in a hybridization buffer comprising 0.001% sodium dodecyl sulfate (SDS; Sigma Aldrich, Darmstadt, Germany), 0.1 M NaCl (Centralchem, Bratislava, Slovakia), and 1 mM EDTA (Centralchem) for 30 min at 37 °C. The resultant heteroduplexes, control ASC1R (1012 molecules), and RdRp sequence (1013 molecules) were then loaded onto a 6% polyacrylamide gel and subjected to electrophoresis at 110 V (40 min, room temperature). Afterwards, nucleic acids were stained with GelRed Nucleic Acid Stain (Millipore, Darmstadt, Germany) for 20 min and visualized at 590 nm, and Cy5-labeled ASC1R was visualized at 680 nm, both using a ChemiDoc MP instrument (Bio-Rad Laboratories, Hercules, CA, USA).
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