Skov3

Manufactured by American Type Culture Collection

Sourced in United States, China, United Kingdom, Germany, Sweden, Canada, Italy, Australia, Israel, Belgium, Cameroon

SKOV3 is a cell line derived from a human ovarian adenocarcinoma. It is commonly used in scientific research as a model for the study of ovarian cancer.

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Lab products found in correlation

1 282 protocols using skov3

Ovarian Cancer Cell Culture Protocols

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All ovarian cancer cell lines were maintained in RPMI-1640 media (Sigma-Aldrich) supplemented with 10% Foetal calf serum (FCS, First link), 0.2 mM L-Glutamine (Gibco), penicillin 50 U ml⁻¹, and streptomycin 50 μg ml⁻¹ (Gibco). HEK293T (Human Embryonic Kidney) cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM) supplemented as above. HCT116 were grown in McCoy’s medium supplemented as above. All cell lines were cultured at 37 °C in a 5% CO₂ humidified incubator. Transduced cells were maintained in full medium supplemented with Puromycin dihydrochloride (Gibco) (10 μg ml⁻¹ for SKOV3, 5 μg ml⁻¹ for PEA1, and 1 μg ml⁻¹ for PEO1 and HCT116).
SKOV3, HEK293T and HCT116 cells were purchased from ATCC. PEA1 and PEO1 cells were acquired from Cancer Research UK. All cells were tested regularly to exclude mycoplasma infection.

Birtley J.R., Alomary M., Zanini E., Antony J., Maben Z., Weaver G.C., Von Arx C., Mura M., Marinho A.T., Lu H., Morecroft E.V., Karali E., Chayen N.E., Tate E.W., Jurewicz M., Stern L.J., Recchi C, & Gabra H. (2019). Inactivating mutations and X-ray crystal structure of the tumor suppressor OPCML reveal cancer-associated functions. Nature Communications, 10, 3134.

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Culturing Human Cell Lines

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Human ovarian adenocarcinoma
(SK-OV-3),
leukemia (CCRF-CEM), and embryonic kidney (HEK 293T) cells were purchased
from American Type Culture Collection. The SK-OV-3 and HEK 293T cells
were grown in Eagle’s minimum essential medium (EMEM), and
RPMI-1640 medium (ATCC, Manassas, VA) was used for CCRF-CEM cells
in a humidified atmosphere of 5% CO₂ at 37 °C. Both
media were supplemented with fetal bovine serum (FBS, 10%) and penicillin–streptomycin
solution (1%, 10,000 units of penicillin and 10 mg of streptomycin
in 0.9% NaCl).

Oh D., Nasrolahi Shirazi A., Northup K., Sullivan B., Tiwari R.K., Bisoffi M, & Parang K. (2014). Enhanced Cellular Uptake of Short Polyarginine Peptides through Fatty Acylation and Cyclization. Molecular Pharmaceutics, 11(8), 2845-2854.

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Culturing Breast and Ovarian Cancer Cell Lines

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Human adherent cell lines SKBR3 (breast adenocarcinoma), MDA-MB-231 (breast adenocarcinoma), SKOV3 (ovarian adenocarcinoma) were purchased from American Type Cell Culture (ATCC, Manassas, VA, USA). SKBR3 and SKOV3 cells were maintained in McCoy’s 5A culture medium (ATCC) supplemented with 20% (v/v) fetal bovin serum (FBS, Gibco, ThermoFisher Scientific, Gent, Belgium). Both cell lines were cultured in a humidified incubator at 37 °C in the presence of 5% CO₂ in air. MDA-MB-231 cells were maintained in Leibovitz’s L-15 culture medium (ATCC) supplemented with 10% (v/v) FBS (Gibco) and 100 Units/mL penicillin and 100 µg/mL streptomycin (Gibco). MDA-MB-231 cells were cultured in a humidified incubator at 37 °C in 100% air. All cell lines were dissociated using 5 mM ethylenediaminetetraacetic acid (EDTA, Sigma-Aldrich, St. Louis, MO, USA).

Gijs M., Penner G., Blackler G.B., Impens N.R., Baatout S., Luxen A, & Aerts A.M. (2016). Improved Aptamers for the Diagnosis and Potential Treatment of HER2-Positive Cancer. Pharmaceuticals, 9(2), 29.

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Ovarian Cancer Cell Lines Protocol

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Ovarian cancer cell lines OVCAR-3, Caov-3, SW626, and SK-OV-3 cell lines were purchased from American Type Culture Collection (Manassas, VA, USA). A2780 cell line was purchased from ECACC (UK). OVCAR-3 cells were cultured in RPMI-1640 Medium (ATCC, #30-2001) with 0.01 mg/ml bovine insulin and 20% fetal bovine serum (FBS, HyClone; GE Healthcare Life Science, Logan, UT, USA). Caov-3 cells were fostered in Dulbecco’s Modified Eagle’s Medium (DMEM, ATCC, #30-2001) with 10% FBS. SW626 cells were incubated in Leibovitz’s L-15 Medium (ATCC, #30-2008) with 10% FBS. SK-OV-3 cells were trained in McCoy’s 5a Medium (ATCC, #30-2007) with 10% fetal bovine serum. A2780 cells were planted in RPMI-1640 Medium (ATCC, #30-2001) containing 10% FBS. 100 U/mL penicillin and 100 μg/mL streptomycin were added to all mediums. A2780, OVCAR-3, Caov-3, and SK-OV-3 cells were maintained in an incubator at 37°C with 5% CO₂. SW626 cells were kept in an incubator at 37°C. SRT2183 (#HY-19759), SB203580 (#HY-10256), and Torin 1 (#HY-13003) were obtained from Med Chem Express (USA), chloroquine (#C6628), and rapamycin (#V900930) were purchased from Sigma (USA).

Sun T., Hu Y., He W., Shang Y., Yang X., Gong L., Zhang X., Gong P, & Yang G. (2020). SRT2183 impairs ovarian cancer by facilitating autophagy. Aging (Albany NY), 12(23), 24208-24218.

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Cell Line Characterization and Culture

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LNCAP (RRID:CVCL_0395), DU145 (NCI-DTP catalog no. DU-145, RRID:CVCL_0105), and SKOV3 (NCI-DTP catalog no. SKOV-3, RRID:CVCL_0532) cells were obtained from ATCC. The DLD1 wild-type (RRID:CVCL_0248) and BRCA2^−/− (RRID:CVCL_HD57) cell lines were purchased from Horizon Discovery. Cell line identification (short tandem repeat typing) was validated using the CellCheck assay (IDEXX Bioanalytics). All cell lines were validated free of virus and Mycoplasma contamination using the MycoSEQ assay (Thermo Fisher Scientific) or STAT-Myco assay (IDEXX Bioanalytics). All cell lines were grown according to supplier instructions. LNCAP, DU145, and DLD1 were grown in RPMI1640 growth media (Corning 17-105-CV) supplemented with 10% FBS and 2 mmol/L glutamine. SKOV3 were grown in McCoy's 5A (Modified) Medium (Thermo Fisher Scientific 16600082). Olaparib and AZD0156 (ATM inhibitor, ATMi) were made by AstraZeneca, carboplatin and cisplatin were bought from Tocris Bioscience (catalog no. 2626 and 15663-27-1). Olaparib, ATMi, and carboplatin were all solubilized in DMSO at 10 mmol/L stock concentration. cisplatin was solubilized in an aqueous solution at 1.67 mmol/L.

Jamal K., Galbiati A., Armenia J., Illuzzi G., Hall J., Bentouati S., Barrell D., Ahdesmäki M., O'Connor M.J., Leo E, & Forment J.V. (2022). Drug–gene Interaction Screens Coupled to Tumor Data Analyses Identify the Most Clinically Relevant Cancer Vulnerabilities Driving Sensitivity to PARP Inhibition. Cancer Research Communications, 2(10), 1244-1254.

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Cytotoxicity of Paclitaxel and Fenofibric Acid in Ovarian Cancer

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The ovarian cancer cell lines SKOV-3 and CAOV-3 were obtained from the ATCC, Manassas, VA. SKOV-3 and CAOV-3 cells were cultured in RPMI1640 supplemented with 10% FBS. Cells were plated in 12-well plates (1 × 10⁵ cells/well). When they reached approximately 50% confluence, the cells were treated for 2 days with paclitaxel (PAC), fenofibric acid (FA) or their combination at concentrations detailed in legend of Figure 8. The attached cells were trypsinized and cell numbers/well were quantified with a Coulter counter.

Caillaud M., Patel N.H., Toma W., White A., Thompson D., Mann J., Tran T.H., Roberts J.L., Poklis J.L., Bigbee J.W., Fang X., Gewirtz D.A, & Damaj M.I. (2020). A Fenofibrate Diet Prevents Paclitaxel-Induced Peripheral Neuropathy in Mice. Cancers, 13(1), 69.

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Culturing SKOV3, NHDF, and OVCAR3 Cell Lines

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SKOV3 and NHDF cell lines were purchased from ATCC, and SKOV3 was cultured in 10% FBS and 1% PS containing McCoy's 5a Medium. NHDF cells were cultured in 10% FBS and 1% PS containing DMEM Medium. The OVCAR3 cell line was gifted from the laboratory of Research Program in Systems Oncology at the University of Helsinki and cultured in 10% FBS, 1% PS, and 0.01 mg/mL bovine insulin containing RPMI-1640 Medium. All cells were maintained in the incubator of 37 °C and 5% CO₂ atmosphere.

Ma X., Zhou W., Zhang R., Zhang C., Yan J., Feng J., Rosenholm J.M., Shi T., Shen X, & Zhang H. (2023). Minimally invasive injection of biomimetic Nano@Microgel for in situ ovarian cancer treatment through enhanced photodynamic reactions and photothermal combined therapy. Materials Today Bio, 20, 100663.

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Cultivation of Human Ovarian Cancer SKOV-3 Cells

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The human ovarian cancer cell line SKOV-3 used in this study was purchased from ATCC (Rockville, MD, USA). The human ovarian cancer cell line SKOV-3 was cultured in McCoy’s 5A Modified Medium with L-Glutamine (ATCC, Rockville, MD, USA) supplemented with 10% fetal bovine serum, 100 U/ml penicillin and 100 µg/ml streptomycin at 37°C in an atmosphere of 5% CO₂ in air. SKOV-3 cells were seeded at a density of 10⁶ cells/ml in 24-well plates (PAA Laboratories GmbH, Pasching, Austria). Passaged 4–6 times, 70–80% confluence SKOV-3 cells were used for further experiments. Cell viability was consistently > 95% as determined by trypan blue staining.

Mielczarek-Palacz A., Sikora J., Kondera-Anasz Z., Mickiewicz P, & Mickiewicz A. (2015). Effect of Th1/Th2 cytokine administration on proinflammatory SKOV-3 cell activation. Archives of Medical Science : AMS, 12(6), 1337-1347.

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Cultivation of Ovarian Cancer Cell Lines

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Human ovarian cancer cell lines HEY, HEY A8, A2780, SKOV3 and SKOV3 ip1[43 (link)] were purchased from ATCC. A2780/CDDP and an immortalized normal human ovarian epithelial cell line (T29) were maintained in the laboratory of cancer Institute, Fudan University Shanghai Cancer Center. HEY A8, A2780/CDDP and SKOV3 ip1 derived from HEY, A2780 and SKOV3, respectively, are resistant to cisplatin or paclitaxel [44 (link), 45 (link)]. Cells were routinely cultured with RPMI 1640 supplemented with 10% fetal bovine serum (FBS), antibiotics (100U/mL penicillin and 100μg/mL streptomycin) in a humidified incubator at 37°C and 5% CO₂. Both RPMI 1640 medium and fetal bovine serum (FBS) were purchased from Thermo Scientific.

Li S., Yang L., Wang J., Liang F., Chang B., Gu H., Wang H., Yang G, & Chen Y. (2016). Analysis of the chemotherapeutic effects of a propadiene compound on malignant ovarian cancer cells. Oncotarget, 7(35), 57145-57159.

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Culturing Four Epithelial Ovarian Cancer Cell Lines

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Four human epithelial ovarian cancer (EOC) cell lines (SKOV3, CAOV3, A2780, and ES-2) were used in this study. The CAOV3 was obtained from the Korean Cell Line Bank (Seoul, Korea). The SKOV3, A2780, and ES-2 cells were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). The SKOV3 and ES-2 cells were grown with McCoy's 5A Medium (ATCC) containing 10% fetal bovine serum (FBS; Life Technologies, Grand Island, NY, USA), 1.5 mM L-glutamine, 100 U/mL penicillin, and 100 mg/mL streptomycin. CAOV3 was grown with Dulbecco's modified Eagle's medium (DMEM, Life Technologies) containing 10% FBS (Life Technologies), 100 U/mL penicillin and 100 mg/mL streptomycin. A2780 was grown with Roswell Park Memorial Institute medium 1640 (RPM I1640, Life Technologies) containing 10% FBS (Life Technologies), 25 mM HEPES (4-(2-hydroxyethyl)-1piperazineethanesulfonic acid), 100 U/mL penicillin, and 100 mg/mL streptomycin. Cells were grown in a 100 mm dish at 37°C and 5% CO 2 . The cell growth and morphology were routinely monitored.

Ouh Y.T., Cho H.W., Lee J.K., Choi S.H., Choi H.J, & Hong J.H. (2019). CXC chemokine ligand 1 mediates adiponectin-induced angiogenesis in ovarian cancer. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 42(4).

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