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Fluoview fv500 laser scanning confocal system

Manufactured by Olympus
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Sourced in Japan

The Fluoview FV500 Laser Scanning Confocal System is a high-performance microscope imaging system designed for advanced fluorescence microscopy applications. The system utilizes multiple lasers and a sophisticated optical scanning mechanism to capture detailed, high-resolution images of fluorescently labeled samples.

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Lab products found in correlation

Dmlb light microscope, by Leica camera (1 mentions) Ix81 inverted microscope, by Olympus (1 mentions) Fl auto cell imaging system, by Leica camera (1 mentions) Sc 52746, by Santa Cruz Biotechnology (1 mentions) Tritc labeled igg, by Santa Cruz Biotechnology (1 mentions)

2 protocols using fluoview fv500 laser scanning confocal system

1

Live-cell Imaging Techniques Used

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Live-cell imaging was performed using an EVOS FL Auto Cell Imaging System or the Leica DMLB light microscope with differential interference contrast optics and a Leica DC300 video camera; a Nikon A1 Plus laser scanning confocal mounted on a Nikon 90i compound microscope (software version: NIS Elements 4.13 Build 914); or an Olympus Fluoview FV500 Laser Scanning Confocal System mounted on an Olympus Inverted IX81 microscope equipped with external photomultiplier tube (PMT), as described in detail, with strain and set-up information, in Supporting Information Methods S7.
Li G., Qi X., Sun G., Rocha R.O., Segal L.M., Downey K.S., Wright J.D, & Wilson R.A. (2020). Terminating rice innate immunity induction requires a network of antagonistic and redox-responsive E3 ubiquitin ligases targeting a fungal sirtuin. The New phytologist, 226(2).
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2

Immunofluorescence Analysis of Kidney TNF-α

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Approximately half of the left kidney of each mouse was subjected to immunofluorescence analysis. Frozen kidney sections were rinsed in phosphate buffered saline (PBS) at room temperature, blocked in 10% control rabbit serum and incubated with TNF-α primary antibody (1:200); sc-52746; Santa Cruz Inc., Dallas, TX, USA). TRITC-labeled IgG (Santa Cruz Inc., USA) was used as a secondary antibody, then the samples were washed three times in PBS, mounted and the fluorescent signals were detected using an Olympus Fluoview FV500 Laser Scanning Confocal System (Tokyo, Japan).
Chen S.M., Chen T.H., Chang H.T., Lin T.Y., Lin C.Y., Tsai P.Y., Imai K., Chen C.M, & Lee J.A. (2020). Methylglyoxal and D-lactate in cisplatin-induced acute kidney injury: Investigation of the potential mechanism via fluorogenic derivatization liquid chromatography-tandem mass spectrometry (FD-LC-MS/MS) proteomic analysis. PLoS ONE, 15(7), e0235849.
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