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3 protocols using ferrous sulfate

1

Bacterial Enzyme Production Protocol

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Bovine serum albumin, tyrosine, thrombin, and standard streptokinase were obtained from Sigma Aldrich (Bengaluru, India). Starch casein agar, ISP broth, glucose, yeast extract, ammonium sulfate, manganese chloride, sodium carbonate, sodium bicarbonate, magnesium sulfate, sodium acetate, sodium chloride, sodium hydroxide, and ferrous sulfate were purchased from Hi Media laboratories (Mumbai, India). Copper sulfate, Folin–Ciocalteau reagent, potassium dihydrogen phosphate, trichloroacetic acid (TCA), hydrochloric acid (HCl), ethylene-diaminetetraacetic acid (EDTA), and Tris-HCl were purchased from Sisco research laboratories (SRL, Mumbai, India).
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2

Anti-inflammatory effects of LPS-induced model

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Pseudomonas aeruginosa LPS (LPS), Dexamethasone and Rolipram from Sigma–Aldrich (USA), Nordihydroguaiaretic acid (NDGA) from Cayman Chemicals (USA), ELISAMAX™ DELUX HUMAN TNF-α and IL-1β ELISA kits from Biolegend (USA), Thermo Scientific Detoxi-Gel Endotoxin Removing Columns from Thermoscientific (USA), Methanol, EDTA from Merck (USA) and Antibiotic solution, EZ™ MTT cell assay kit, Ferrous sulfate, Fetal bovine serum (FBS), HISEP™, Linoleic acid, RPMI 1640, Trypan blue, Tris base and Tween 20 from Himedia Laboratories (India) were purchased.
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3

DPPH Antioxidant Activity Assay Protocol

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1,1-Diphenyl-2-picrylhydrazyl (DPPH), ethylenediaminetetraacetic acid disodium salt dihydrate (EDTA), nitro blue tetrazolium chloride (NBT), hydrogen peroxide (H 2 O 2 ), nicotinamide adenine dinucleotide phosphate (NADPH), ribo avin, nicotinamide adenine dinucleotide (NADH), methionine, phenazine methosulfate (PMS), Triton X-100, guaiacol, glutathione, ascorbic acid, gallic acid, rutin, and sodium salicylate were provided by Sigma-Aldrich, Merck. Ferrous sulfate (FeSo 4 ), Tris HCl, sodium phosphate, ammonium molybdate, ferric chloride (FeCl 3 ), sodium carbonate, Folin-Ciocalteu reagent, sodium carbonate (Na 2 CO 3 ), ammonia solution (NH 3 ), sulfuric acid (H 2 SO 4 ), hydrochloric acid (HCl), Fehling's solutions A and B, aluminum chloride (AlCl 3 ), mercury potassium iodide, sodium hydroxide (NaOH), isoamyl alcohol, glacial acetic acid, nitric acid, methanol, chloroform, ethanol and hexane were purchased from Himedia.
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