Sulforhodamine b salt

Manufactured by Merck Group

Sourced in United States

Sulforhodamine B salt is a synthetic fluorescent dye commonly used in biochemical and cell biology research. It functions as a colorant and has applications in various laboratory techniques, such as protein quantification and cell viability assays. The dye exhibits excitation and emission wavelengths suitable for fluorescence-based measurements.

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Sulforhodamine B sodium salt (SRB) Sulforhodamine B (SRB) sodium salt solution Sulforhodamine B sodium salt assay Sulforhodamine B sodium salt Sulforhodamine B

5 protocols using sulforhodamine b salt

Acquisition and Preparation of Compounds for Cell and Animal Studies

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Authentic (−)-deguelin, rapamycin for in vitro studies and enzalutamide/MDV3100 were purchased from Cayman Chemical Company (Ann Arbor, MI, USA). rapamycin for animal studies was purchased from LC Laboratories (Woburn, MA, USA). R1881 was purchased from Perkin Elmer (Waltham, MA, USA). Sulforhodamine B salt, paclitaxel, 17-AAG, crystal violet, Trizma, Dulbecco’s phosphate-buffered saline (DPBS), HEPES, hydrocortisone, insulin, phenylmethanesulfonyl fluoride (PMSF), carboxymethyl cellulose, Tween-80, polyethylene glycol 400 and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Compounds for cell treatments were dissolved in DMSO and stored at −20°C. Compounds for in vivo studies were stored as stock solutions in DMSO (enzalutamide) or ethanol (rapamycin) at −20°C and diluted immediately before use.

Robles A.J., Cai S., Cichewicz R.H, & Mooberry S.L. (2016). Selective activity of deguelin identifies therapeutic targets for androgen receptor-positive breast cancer. Breast cancer research and treatment, 157(3), 475-488.

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Analytical Characterization of Compounds

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Sulforhodamine B salt, Trizma, Dulbecco’s phosphate-buffered saline (DPBS), phenylmethanesulfonyl fluoride (PMSF), dimethyl sulfoxide (DMSO), crystal violet, and Kolliphor^® EL were purchased from Sigma-Aldrich (St. Louis, MO, USA). Acetic acid was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Abemaciclib-mesylate was provided by Dr. Peter Houghton. The compounds used for in vitro cell treatments were dissolved in DMSO and stored at −20 °C. ATXII for in vivo studies was diluted in 50:50 DMSO:Kolliphor^® EL, stored at −20 °C, and diluted 1:10 in DPBS immediately prior to use. LCMS analyses were performed on a Shimadzu UFLC system with a quadrupole mass spectrometer using a Phenomenex Kinetex C18 column (3.0 mm × 75 mm, 2.6 μm) and a CH₃CN-H₂O (0.1% HCOOH) gradient solvent system. NMR spectra were obtained on a Varian a spectrometer (500 MHz for ¹H and 125 MHz for ¹³C) using acetone-d₆ (Aldrich) as the solvent. HPLC was performed on a Waters System equipped with a 1525 binary HPLC pump coupled to a 2998 PDA detector with a Phenomenex C18 column (21.2 × 250 mm or 10 × 250 mm, 5 μm).

Robles A.J., Dai W., Haldar S., Ma H., Anderson V.M., Overacker R.D., Risinger A.L., Loesgen S., Houghton P.J., Cichewicz R.H, & Mooberry S.L. (2021). Altertoxin II, a Highly Effective and Specific Compound against Ewing Sarcoma. Cancers, 13(24), 6176.

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Cytotoxicity Evaluation of CBD and CBD-Capped NPs

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The following materials were used in the cytotoxicity
evaluation
of CBD and CBD-capped NPs in HaCaT cells: HaCaT cell line (human epidermal
keratinocytes; American Type Culture Collection); CBD (Endoca BV Netherlands);
Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine
serum (FBS), phosphate-buffered saline (PBS) (Gibco—Thermo
Fisher Scientific, South Africa); and acetic acid, dimethyl sulfoxide
(DMSO), ethylenediaminetetraacetic acid (EDTA), fungizone, penicillin,
sodium hydroxide (NaOH), streptomycin, sulforhodamine B salt, trichloroacetic
acid (TCA), trisaminomethane (Tris), trypan blue, and trypsin (Sigma-Aldrich,
South Africa).

Josiah A.J., Pillai S.K., Cordier W., Nell M., Twilley D., Lall N, & Ray S.S. (2021). Cannabidiol-Mediated Green Synthesis, Characterization, and Cytotoxicity of Metal Nanoparticles in Human Keratinocyte Cells. ACS Omega, 6(43), 29078-29090.

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Prostate Cancer Cell Line Analysis

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Prostate cancer cell lines, AR− (PC3 and DU145 cells) and AR+ (LnCAP cells) and mouse embryonic fibroblasts (NIH 3T3 cells) were obtained from American Type Culture Collection (Manassas, VA, USA). The epithelial prostate cell line immortalized with SV40 (PNT1A) was gifted from the Department of Anatomy, University of Otago. Primary antibodies to acetyl H3, cyclin D, cyclin B, p21, p27kip1, and bcl-2 were purchased from Cell Signaling Technology (Danvers, MA, USA). β-tubulin, β-actin, Dulbecco’s modified Eagle’s medium (DMEM) nutrient mixture Ham’s F-12, sulforhodamine B salt and propidium iodide (PI) were purchased from Sigma-Aldrich (Auckland, New Zealand). Acrylamide, bisAcrylamide, sodium dodecylsulfate and PVDF membrane were purchased from Bio-Rad Laboratories (Hercules, CA, USA). Complete mini-EDTA-free protease inhibitor was purchased from Roche Diagnostics Corporation (Mannheim, Germany). Annexin V APC was obtained from BD Pharmingen (San Jose, CA, USA). FxCycle PI RNase staining solutions were ordered from Life Technologies (Christchurch, New Zealand). The Histone Deacetylase (HDAC) Activity Assay Kit (Fluorometric) was purchased from Abcam (Melbourne, Australia). Jazz90 and Jazz167 were synthesized as previously described [23 (link)]. SAHA was obtained from AK Scientific (Union City, CA, USA).

Rana Z., Tyndall J.D., Hanif M., Hartinger C.G, & Rosengren R.J. (2021). Cytostatic Action of Novel Histone Deacetylase Inhibitors in Androgen Receptor-Null Prostate Cancer Cells. Pharmaceuticals, 14(2), 103.

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Sulforhodamine B Cytotoxicity Assay

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Sulforhodamine B salt, Trizma, Dulbecco’s phosphate-buffered saline (DPBS), phenylmethanesulfonyl fluoride (PMSF) and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Compounds for cell treatments were dissolved in DMSO and stored at −20°C. Maximiscin for in vivo studies was stored as a stock solution in 50:50 DMSO:Kolliphor^® EL at −20°C and diluted in DPBS immediately prior to use.

Robles A.J., Du L., Cichewicz R.H, & Mooberry S.L. (2016). Maximiscin Induces DNA damage, Activates DNA Damage Response Pathways and Has Selective Cytotoxic Activity against a Subtype of Triple-Negative Breast Cancer. Journal of natural products, 79(7), 1822-1827.

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