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αcd8α pe

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αCD8α-PE is a fluorescently labeled antibody that binds to the CD8α (cluster of differentiation 8 alpha) receptor. It is commonly used in flow cytometry applications to identify and quantify CD8+ T cells.

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Lab products found in correlation

Facscanto analyzer, by BD (2 mentions) αpd 1, by Santa Cruz Biotechnology (2 mentions) Abd pe, by Santa Cruz Biotechnology (2 mentions) αb220 apc, by BioLegend (2 mentions) αcd4 pe, by BioLegend (2 mentions)

2 protocols using αcd8α pe

1

Immunomodulation of NOD and C57BL6 Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ten-week-old female NOD mice were randomly assigned into 4 groups and treated with one dose of αPD-1-ABD-PE (5 mg/kg), a mixture of αPD-1 (scFv, 2.5 mg/kg) and ABD-PE (2.5 mg/kg), PBS, or CP (200 mg/kg) (Santa Cruz Biotechnology) intraperitoneally. These mice were euthanized 24 hours later. Blood samples and splenocytes were collected from the mice, and red blood cells in these samples were lysed with the Ammonium-Chloride-Potassium (ACK) Lysing Buffer. The remaining cells in the samples were pelleted by a centrifugation at 300 g for 5 minutes, and stained by αB220-APC (Biolegend), αCD4-PE (Biolegend), or αCD8α-PE (Santa Cruz Biotechnology, Inc). The B220+, CD4+, and CD8+ fractions in the pellets were analyzed by flow cytometry on a BD FACSCanto Analyzer. The same experiment was conducted on 10-week-old female C57BL6 mice.
Zhao P., Wang P., Dong S., Zhou Z., Cao Y., Yagita H., He X., Zheng S.G., Fisher S.J., Fujinami R.S, & Chen M. (2019). Depletion of PD-1-positive cells ameliorates autoimmune disease. Nature biomedical engineering, 3(4), 292-305.
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2

Immunomodulation of NOD and C57BL6 Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ten-week-old female NOD mice were randomly assigned into 4 groups and treated with one dose of αPD-1-ABD-PE (5 mg/kg), a mixture of αPD-1 (scFv, 2.5 mg/kg) and ABD-PE (2.5 mg/kg), PBS, or CP (200 mg/kg) (Santa Cruz Biotechnology) intraperitoneally. These mice were euthanized 24 hours later. Blood samples and splenocytes were collected from the mice, and red blood cells in these samples were lysed with the Ammonium-Chloride-Potassium (ACK) Lysing Buffer. The remaining cells in the samples were pelleted by a centrifugation at 300 g for 5 minutes, and stained by αB220-APC (Biolegend), αCD4-PE (Biolegend), or αCD8α-PE (Santa Cruz Biotechnology, Inc). The B220+, CD4+, and CD8+ fractions in the pellets were analyzed by flow cytometry on a BD FACSCanto Analyzer. The same experiment was conducted on 10-week-old female C57BL6 mice.
Zhao P., Wang P., Dong S., Zhou Z., Cao Y., Yagita H., He X., Zheng S.G., Fisher S.J., Fujinami R.S, & Chen M. (2019). Depletion of PD-1-positive cells ameliorates autoimmune disease. Nature biomedical engineering, 3(4), 292-305.
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