The concentration of murine CXCL1, CXCL2, and IL-6 in mouse plasma was measured using a mouse premixed multi-analyte kit (R&D systems, Minneapolis, MN, USA) according to the manufacturer’s instructions. Fluorescence intensities were measured using a MAGPIX multiplexing system (Luminex Corporation, Austin, TX, USA). The concentration of murine C-reactive protein (CRP) in mouse plasma was measured by the enzyme-linked immunosorbent assay (ELISA) using CRP ELISA kit (R&D systems, Minneapolis, MN, USA) according to the manufacturer’s instructions. Optical densities were measured at 450 nm using a Bio-Rad Model 550 microplate reader (Bio-Rad Laboratories, Irvine, CA, USA). The concentration of analytes in plasma was calculated from standard curves generated by a curve-fitting program.
Crp elisa kit
Manufactured by R&D Systems
Sourced in United States
The CRP ELISA kit is a laboratory test used to measure the concentration of C-reactive protein (CRP) in a sample. CRP is an acute-phase protein produced by the liver in response to inflammation. The kit utilizes the enzyme-linked immunosorbent assay (ELISA) technique to quantify the CRP levels in a given sample.
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Lab products found in correlation
4 protocols using crp elisa kit
1
Multiplex Cytokine Analysis in Mouse Plasma
2
Measurement of Cytokines and CRP
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According to the kit instructions, the concentrations of IL-1β, IL-6, and IL-8 in GCF and serum as well as CRP in GCF were measured using Human High-Sensitivity T Cell Magnetic Bead Panel (Luminex Corporation, Austin, TX, USA). Furthermore, the concentration of CRP in serum was measured using a CRP ELISA kit (R&D Systems, Minneapolis, MN, USA).
3
CRP Quantification in Hepa1c1c-7 Cells
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The concentrations of CRP in the supernatants harvested from the culture in Hepa1c1c-7 cell were determined using a CRP ELISA kit (R&D Systems Inc., MN, USA) according to the manufacturer instructions. Concentrations of CRP were measured at 450 nm using ELISA. The lower limit of sensitivity for each ELISA was ≤ 5 pg/mL.
4
Plasma C-reactive Protein Measurement
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Plasma C-reactive protein (CRP) was assessed using a specific CRP ELISA kit (R&D systems, Pharmaco, Auckland, New Zealand), calculated as mg/L and presented as % pre-exercise values.
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