Image analysis system
The Image analysis system is a software tool designed to capture, process, and analyze digital images. It provides advanced image processing capabilities, enabling users to perform tasks such as image enhancement, segmentation, and quantitative analysis. The core function of this system is to facilitate the extraction of meaningful information from digital images, supporting research and analysis across various fields.
Lab products found in correlation
19 protocols using image analysis system
Hematoxylin and Eosin Staining Protocol
Collagen Gel Contraction Assay with Lipoaspirate
Blank: collagen with culture medium (without cells and stimuli);
Ctr: collagen with cells without stimuli;
Ctr+: collagen with cells + TGFβ1 5 ng/mL;
LIPO: collagen with cells + 15% v/v of LipoCM.
Transwell Invasion Assay for CD63 Modulation
The non-invading cells in the upper side of the filter were then gently removed with a soft cotton swab, and the cells that had invaded to the lower side of the filter were fixed with 4% paraformaldehyde at room temperature for 30 min and stained with 1% crystal violet at 37°C for 15 min (Sigma-Aldrich; Merck KGaA). The number of cells in three randomly selected fields was counted with an Image Analysis System (version 3.3.0; Leica Microsystems GmbH), and these numbers are expressed as the average number of migrating cells.
Cardiac Collagen Quantification Protocol
Quantifying Cardiac Calcium Content
Quantifying Renal Collagen Content
Lymphocyte Karyotyping by G-Banding
Quantifying Vascular Re-endothelialization Post-Injury
Rats were sacrificed by removing the common carotid artery and cultured in PBS. The common carotid arteries were placed into a petri dish containing PBS and were cut longitudinally by removing excess muscles and connective tissue. These arteries were rinsed again with PBS and then plated on glass slide. Images were captured using a micro-camera to determine the re-endothelialization of the injured vessels. The total area of the vessels and the area of the Evans blue stained area were measured with Lecia Qwin image analysis system. The percentage of re-endothelialization of the injured vessels was calculated by the following equation: (the total area of injured blood vessel - the area of the Evans blue stained area)/the total area of the injured blood vessel.
Immunohistochemical Analysis of DEC2, Ki-67, and NR2F1
Immunohistochemical Analysis of Collagen I and III
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