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Anti caspase 8

Manufactured by OriGene
Sourced in United States

Anti-caspase-8 is a laboratory reagent used for the detection and quantification of caspase-8, an enzyme involved in the initiation of apoptosis (programmed cell death). This product can be used in various cell-based assays and biochemical experiments to study the role of caspase-8 in cellular processes.

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2 protocols using anti caspase 8

1

Apoptotic and Cell Cycle Signaling Pathways in Cellular Response

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Fetal bovine serum (FBS; US origin) was purchased from HyClone Laboratories Inc., South Logan, UT, USA. Anti-caspase-3, anti-caspase-8, anti-PARP, anti-BID, anti-Bax, anti-COX-2 and anti-beta-actin antibodies were purchased from OriGene Technologies, Inc., Rockville, MD, USA. Anti-p53, anti-Bcl-2 (NT), anti-caspase-9 and anti-iNOS antibodies were purchased from AnaSpec, Inc., Fremont, CA, USA. Anti-Cdc25C, anti-CDK1, anti-CDK2, anti-Cyclin A1 and anti-Cyclin B1 antibodies were purchased from Bioss, Inc., Woburn, MA, USA. Anti-p21 (WAF1, Cip1) was purchased from eBioscience, San Diego, CA, USA. Anti-cyclin D1 and anti-cdk-4 antibodies were purchased from Cell Signaling Technology, Inc., Danvers, MA, USA. Cell Proliferation Reagent WST-1 and the Annexin-V-FLUOS Kit were purchased from Roche Diagnostics, Mannheim, Germany. Alkaline phosphatase-conjugated anti-Rabbit secondary antibody was purchased from RockLand Immunochemicals Inc., Gilbertsville, PA, USA. Z-IETD-FMK (caspase-8 inhibitor) and Z-LEHD-FMK (caspase-9 inhibitor) were purchased from Abcam, Cambridge, MA, USA. All the other chemicals and solvents are of analytical or molecular biology grade and procured locally.
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2

Apoptosis Signaling Pathway Analysis

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Cell proliferation reagent WST-1 and Annexin-V-FLUOS kit were procured from Roche diagnostics, Mannheim, Germany. US-origin fetal bovine serum (FBS) was purchased from HyClone Laboratories Inc., Utah, USA. All the other chemicals and solvents are of minimum analytical or molecular biology grade and procured locally. Anti-B-cell lymphoma (Bcl-2) (NT), anti-caspase-9 and anti-p53 antibodies were purchased from AnaSpec, Inc., USA. Anti-poly (adenosine diphosphate-ribose) polymerase (PARP), anti-caspase-3, anti-caspase-8, anti-Bcl-2-associated X (Bax), anti-BH3 interacting-domain (Bid), anti-pERK-1/2, anti-pJNK, anti-p38 and anti-beta-actin antibodies were purchased from OriGene Technologies Inc., Rockville, USA. Alkaline phosphatase conjugated anti-Rabbit secondary antibody was purchased from RockLand Immunochemicals Inc., Gilbertsville, USA.
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