Anti p65

Triptolide (TP) (purity >98%) was obtained from Purcell biology (Chengdu, China). Clomethiazole (CMZ) (purity >98%) and pyrazole (PZ) (purity>98%) were purchased from Aladdin (Shanghai, China). 3-(4, 5-dimethyl-2thiazolyl)-2, 5-diphenylterazolium bromide (MTT) was obtained from Beyotine Biotechnology Research Institute (Shanghai, China). Anti-CYP2E1 and anti-β-actin were purchased from Proteintech (Shanghai, China). Anti-P65 was obtained from Wanleibio (Shengyang, China). Antiβ-actin antibody and secondary antibodies for goat were obtained from ZSGBBIO (Beijing, China). Primary Antibody Dilution Buffer, RIPA Lysis, Penicillin-Streptomycin Solution and Extraction Buffer and PMSF were obtained from Beyotime (Hangzhou, China). Transcriptor First Strand cDNA Synthesis Kit, PrimeSTAR, and TB Green ® Premix Ex TaqÔ II were obtained from TaKaRa (Shiga, Japan). LipofectamineTM2000 and Trizol were purchased from Invitrogen (Carlsbad, CA, USA). CYP2E1, TNF-α, IL-6, (p-P65), and β-actin primers were obtained from Sangon Biotech (Shanghai, China). ROS assay kit and BCA protein assay kit were obtained from Beyotime (Hangzhou, China). The alanine transaminase (ALT), aspartate aminotransferase (AST), malondialdehyde (MDA), superoxide dismutase (SOD), Catalase (CAT) and glutathione (GSH) determination kits were obtained from Jiancheng Bioengineering Institute of Nanjing (Nanjing, China).