Trehalose 6 6 dibehenate tdb

Rabbit anti‐mouse thymocyte serum was generated by the Hybridoma Core at the Cleveland Clinic Lerner Research Institute as previously described.⁴ Heart allograft recipients were treated with mATG (0.5 mg i.p.) on days 0 and 4 posttransplant, and non‐transplanted mice were injected with 0.5 mg mATG i.p. on days 0 and 4 of the experiment. For CD4 T cell depletion, anti‐mouse CD4 mAb (clones GK1.5 and YTS191, BioXCell) were injected i.p. on days −3, −2, −1 relative to mATG treatment, 200 μg each per animal per day. When indicated, the recipients were treated i.v. with 1 mg anti‐CD154 mAb MR1 1 day prior to the surgery or with 0.1 mg agonistic anti‐CD40 mAb FGK4.5 on days 0 and 1 posttransplant (both from BioXCell). For the administration of synthetic Mincle agonist, non‐transplanted B6 mice treated with 0.5 mg mATG on days 0 and 4 were additionally injected with trehalose‐6,6‐dibehenate (TDB, InvivoGen) at 4 mg/kg i.p. twice weekly for the experiment duration starting on day 2.