Mice bearing orthotopic MB49-luciferase tumors were administered 3 mg of D-luciferin bioluminescent substrate (PerkinElmer) via intraperitoneal injection. Prior to imaging, mice were anesthetized with inhalational isoflurane, and abdominal hair was removed via shaving. Images were subsequently acquired 20 min after luciferin injection using an IVIS Spectrum in vivo imaging system (PerkinElmer) with a 10 s exposure time. Total luminescence counts were quantified across a standardized region of interest centered on the bladder area using Living Image analysis software (PerkinElmer).
D luciferin bioluminescent substrate
Manufactured by PerkinElmer
Sourced in United States
D-luciferin is a bioluminescent substrate used in various research applications. It is a chemically synthesized compound that emits light when combined with the enzyme luciferase in the presence of oxygen. The light emission is a result of the luciferin-luciferase reaction, and it can be used to detect and quantify the presence of luciferase-expressing cells or organisms.
Automatically generated - may contain errors
3 protocols using d luciferin bioluminescent substrate
1
In Vivo Bioluminescent Imaging of Bladder Tumors
2
Cytotoxicity Assay with Luc-Raji Cells
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Cytotoxicity were tested using luciferase‐labelled target cells (Luc‐Raji) as described by Melaiu et al.35 Luc‐Raji cells were suspended in RPMI‐1640 medium supplemented with 10% FBS in 96‐well, flat‐bottomed white view plates (Corning). Expanded NK cells suspended in RPMI 1640 with 10% FBS and 400 IU/ml IL‐2 were added at varying effector to target cell ratios as indicated in results section. The plates were incubated for 4 h at 37°C with 5% CO2. At the end of the culture, cell mixture was incubated with equal volume of D‐luciferin bioluminescent substrate (Perkin‐Elmer, Waltham, USA) for 10 min, then measured using a microplate reader (SpectraMax iD3). Viability was calculated by comparing relative luminescent signal from control wells on each plate. All experiments were done in triplicates.
3
Bioluminescent Tumor Imaging in Mice
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Mice bearing orthotopic MB49-luciferase tumors were administered 3 mg of D-luciferin bioluminescent substrate (PerkinElmer) via intraperitoneal injection. Prior to imaging, mice were anesthetized with inhalational isoflurane and abdominal hair was removed via shaving. Images were subsequently acquired 20 min after luciferin injection using an IVIS Spectrum in vivo imaging system (PerkinElmer) with a 10 s exposure time. Total luminescence counts were quantified across a standardized region of interest centered on the bladder area using Living Image analysis software (PerkinElmer).
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