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A10043

Manufactured by LI COR
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Sourced in United States

The A10043 is a compact infrared carbon dioxide (CO2) analyzer designed for laboratory and field applications. It is capable of measuring CO2 concentrations with high accuracy and precision. The device features a non-dispersive infrared (NDIR) sensor and is optimized for reliable and continuous operation.

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Lab products found in correlation

Trizol kit, by Thermo Fisher Scientific (2 mentions) Sigma sab2502119, by Merck Group (2 mentions) A21088, by Thermo Fisher Scientific (2 mentions) Tris glycine sds page, by Bio-Rad (2 mentions) Laemmli sample buffer, by Bio-Rad (2 mentions) Phospho rb ser807 811, by Cell Signaling Technology (1 mentions) Mab cyclin b1, by Santa Cruz Biotechnology (1 mentions) Odyssey clx infrared imager, by LI COR (1 mentions) Gapdh, by Merck Group (1 mentions)

3 protocols using a10043

1

HCMV Infection Dynamics in HFF Cells

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A total of 0.5 × 106 HFF were seeded in 10 cm2 dishes and were either treated with 3MB-PP1, MBV or DMSO as a negative control. On the next day, the cells were infected with the respective HCMV strains with different genomes per cell (4 genomes per cell). On different days post-infection, the cells were harvested, adjusted to 1 × 105 cells and lysed in 2× Laemmli sample buffer. Protein samples were separated on 10% SDS-PAGE (Life Technologies, NP0301BOX, Carlsbad, CA, USA) and transferred to PVDF membranes (Immobilon, ISEQ00010). The filters were probed with specific primary antibodies phospho-Rb (Ser807/811) (Cell Signaling, 8516S), Rb (Cell Signaling, 9309S), pp28 (kindly provided by William Britt), GAPDH (Sigma-Aldrich, G8795), pAb-UL97 (kindly provided by D.M. Coen, Harvard Medical School, Boston, MA, USA), mAb-Cyclin B1 (sc-245, Santa Cruz Biotechnology), Fluorescent-conjugated secondary antibodies (Invitrogen, A10043; Licor,926-32212) were used for detection by using the Odyssey Infrared Imager CLx (LI-COR Biotechnology, Lincoln, NE, USA).
Krämer N., Schütz M., Mato U.G., Herhaus L., Marschall M, & Zimmermann C. (2022). Recombinant Human Cytomegalovirus Expressing an Analog-Sensitive Kinase pUL97 as Novel Tool for Functional Analyses. Viruses, 14(10), 2285.
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2

Quantifying Protein Expression in Ischemic Muscle and BMDMs

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Proteins from ischemic muscle tissue (100–150 mg) were extracted using Trizol kit (Fisher 15596026). Proteins from BMDMs (1.5million/mL) were collected by using Laemmli sample buffer (Bio-Rad, cat.1610737) containing 2-βME; then boiled at 95°C and aliquoted. Aliquots from tissue lysates or cell lysates were separated by Tris/Glycine/SDS–PAGE (Bio-Rad 1610732) and blotted into PVDF membranes. Membranes were blocked in fluorescent blocking buffer (Rockland Immunochemicals, cat.MB070) and probed at 4°C overnight with antibodies against VEGF-A (1:1000; Sigma SAB2502119); cleaved mature IL-1β (1:500; Cell Signaling Technology 52718); β-Actin (1:1000, sc-47778). After multiple washes, membranes were incubated with fluorescent secondary antibodies (Thermo Fisher A21088, 1:5000; Thermo Fisher A10043, 1:5000; and LI-COR 926–32212, 1:5000). Protein bands were quantified by densitometry, using the LI-COR Imager (Odyssey CLx) and normalization to β-Actin.
Mantsounga C.S., Lee C., Neverson J., Sharma S., Healy A., Berus J.M., Parry C., Ceneri N.M., López-Giráldez F., Chun H.J., Lu Q., Sellke F., Choudhary G, & Morrison A.R. (2022). Macrophage IL-1β promotes arteriogenesis by autocrine STAT3- and NF-κB-mediated transcription of pro-angiogenic VEGF-A. Cell reports, 38(5), 110309.
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3

Quantifying Protein Expression in Ischemic Muscle and BMDMs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Proteins from ischemic muscle tissue (100–150 mg) were extracted using Trizol kit (Fisher 15596026). Proteins from BMDMs (1.5million/mL) were collected by using Laemmli sample buffer (Bio-Rad, cat.1610737) containing 2-βME; then boiled at 95°C and aliquoted. Aliquots from tissue lysates or cell lysates were separated by Tris/Glycine/SDS–PAGE (Bio-Rad 1610732) and blotted into PVDF membranes. Membranes were blocked in fluorescent blocking buffer (Rockland Immunochemicals, cat.MB070) and probed at 4°C overnight with antibodies against VEGF-A (1:1000; Sigma SAB2502119); cleaved mature IL-1β (1:500; Cell Signaling Technology 52718); β-Actin (1:1000, sc-47778). After multiple washes, membranes were incubated with fluorescent secondary antibodies (Thermo Fisher A21088, 1:5000; Thermo Fisher A10043, 1:5000; and LI-COR 926–32212, 1:5000). Protein bands were quantified by densitometry, using the LI-COR Imager (Odyssey CLx) and normalization to β-Actin.
Mantsounga C.S., Lee C., Neverson J., Sharma S., Healy A., Berus J.M., Parry C., Ceneri N.M., López-Giráldez F., Chun H.J., Lu Q., Sellke F., Choudhary G, & Morrison A.R. (2022). Macrophage IL-1β promotes arteriogenesis by autocrine STAT3- and NF-κB-mediated transcription of pro-angiogenic VEGF-A. Cell reports, 38(5), 110309.
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