Pmg plasmid

Manufactured by InvivoGen

Sourced in France

The PMG plasmid is a circular DNA molecule used in molecular biology applications. It serves as a vector for the cloning and expression of genetic material in various host organisms.

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Lab products found in correlation

Tip 500 columns, by Qiagen (2 mentions)

2 protocols using pmg plasmid

Plasmid Transfection Protocol for Cell Transduction

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For plasmid transfection, a pCMV-lacz plasmid (Plasmid factory) was used encoding a β-galactosidase protein (LacZ) gene under the control of a CMV promoter. The EEV plasmid was created by incorporating a SFV DNA replicase sequence (kindly donated by Prof Greg Atkins, Virus Group, Department of Microbiology, School of Genetics and Microbiology, Trinity College Dublin). A nuclear localization sequence was also incorporated to allow for more efficient nuclear targeting of the pEEV plasmid (Figure 1). The pMG plasmid was purchased from InvivoGen (Toulouse, France) (Supplementary Figure S1). Plasmids were propagated in Escherichia coli strain Top10 and purified on endotoxin-free Qiagen-tip 500 columns (Qiagen, Manchester, UK).

Forde P.F., Hall L.J., Sadadcharam M., de Kruijf M., O’ Sullivan G.C, & Soden D.M. (2014). Development and characterization of an enhanced nonviral expression vector for electroporation cancer treatment. Molecular Therapy. Methods & Clinical Development, 1, 14012-.

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Engineered Plasmid for Cancer Immunotherapy

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The pMG plasmid was purchased from InvivoGen (Toulouse, France). A version of this plasmid, designated pGT141, containing the murine GMCSF and human B7-1 genes transcriptionally controlled from the hEF1-HTLV and cytomegalovirus promoters, respectively, was designed and cloning was performed on contract by InvivoGen. The EEV plasmid was created by incorporating a Semliki Forest virus DNA replicase sequence (kindly donated by Prof Greg Atkins, Virus Group, Department of Microbiology, School of Genetics and Microbiology, Trinity College, Dublin, Ireland). A nuclear localisation sequence was also incorporated to allow for nuclear targeting.²⁶ The murine GMCSF and human b7.1 genes were also incorporated into the pEEV. Plasmids were propagated in Escherichia coli strain Top10 and purified on endotoxin-free Qiagen-tip 500 columns (Qiagen) All plasmids are described in Supplementary Figure S2.

Forde P.F., Hall L.J., de Kruijf M., Bourke M.G., Doddy T., Sadadcharam M, & Soden D.M. (2014). Non-viral immune electrogene therapy induces potent antitumour responses and has a curative effect in murine colon adenocarcinoma and melanoma cancer models. Gene Therapy, 22(1), 29-39.

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