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C57 6092

Manufactured by Cell Biologics
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Sourced in United States

C57–6092 is a laboratory equipment product. It functions as a cell culture incubator, providing a controlled environment for the growth and maintenance of cells in vitro.

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Lab products found in correlation

H1168, by Cell Biologics (1 mentions) Human ifnα2, by PBL Assay Science (1 mentions) Cyan adp flow cytometer, by Agilent Technologies (1 mentions) Rifn γ, by BioLegend (1 mentions) M1168, by Cell Biologics (1 mentions) Jetprime transfection reagent, by Polyplus Transfection (1 mentions)

2 protocols using c57 6092

1

IFN Effects on Lymphatic Endothelial Cells

Cited 2 times
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Primary mLECs (Cell Biologics, C57–6092) and hLECs (Cell Biologics, H-6092) were cultured in in endothelial cell media (Cell biologics, M1168 and H1168). Flasks and plates were coated with matrigel diluted 1:1000 in media(31 (link)). mLECS were treated with rIFNα (a kind gift from Ross Kedl(32 (link))) and rIFNγ (Biolegend, 714006) at 250 U/mL(33 (link)) for 24 hours (h) before cells were harvested. Cells were then stained with αPDPN and αPD-L1, and acquired on a Cyan ADP flow cytometer (Dako) and analyzed using flowjo software (Treestar). hLECs were treated with human IFNα2 (PBL Assay Science-11100–1) at 500 U/mL(34 (link)) for 24 h and then harvested as above.
Lucas E.D., Finlon J.M., Burchill M.A., McCarthy M.K., Morrison T.E., Colpitts T.M, & Jirón Tamburini B.A. (2018). Type 1 interferon and PD-L1 coordinate lymphatic endothelial cell expansion and contraction during an inflammatory immune response. Journal of immunology (Baltimore, Md. : 1950), 201(6), 1735-1747.
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2

HUVEC and mLEC miR-223 Regulation

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Human umbilical vein endothelial cells (HUVECs) or mouse primary lymphatic endothelial cells (mLECs; C57-6092, Cell Biologics, Chicago, USA) were seeded into 24-well plates (2 × 105/well) and cultured using complete RPMI 1640 medium containing 10% FBS. Upon a cell density of 50–70%, miR-223 mimic (100 nM) and negative control was separately transfected with jetPrime transfection reagent (114–15, Polyplus, France). Cells were collected after 48 h. Then qPCR and Western blot were used to measure expression of miR-223 and target gene ICAM-1.
Liu W., Zhou N., Liu Y., Zhang W., Li X., Wang Y., Zheng R, & Zhang Y. (2021). Mesenchymal stem cell exosome-derived miR-223 alleviates acute graft-versus-host disease via reducing the migration of donor T cells. Stem Cell Research & Therapy, 12, 153.
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