Blood plasma was analyzed for blood urea nitrogen (BUN), calcium, phosphorus, and creatinine using colorimetric assays (Point Scientific, Canton, MI, USA; or Sigma kits; Sigma, St. Louis, MO, USA). Intact PTH was determined by ELISA (Alpco, Salem, NH, USA).
Sigma kits
Manufactured by Merck Group
Sourced in United States
Sigma kits are a range of laboratory products manufactured by Merck Group. They are designed to assist researchers and scientists in various experimental and analytical procedures. The core function of Sigma kits is to provide reliable and consistent tools for tasks such as sample preparation, protein analysis, and nucleic acid extraction, among others. These kits are known for their quality and are used widely in scientific research and development.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using sigma kits
1
Biochemical Markers in Blood Plasma
2
Tracking Blood Pressure in Newborn SHRs and WKYs
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Newborn male SHRs (n = 45) and WKYs (n = 45), were evaluated during their first 8 weeks of life (from birth, i.e. week 0, to week 8). Their blood pressure was taken weekly using the CODA tail-cuff blood pressure system (Kent Scientific Corporation). Weekly, 5 rats per group (SHR and WKY), were sacrificed with a lethal injection of pentobarbital, and their kidneys were removed, decapsulated and weighed. The serum was collected for measuring biochemical parameters. The serum urea and creatinine concentrations were determined using colorimetric assays (Sigma kits).
3
Avian Blood Sample Analysis
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All data obtained were statistically analyzed Collection of blood samples and analysis Blood (2mLs) was collected from three birds of each replicate via wing vein. Samples collected were put in a sterile b o t t l e t u b e c o n t a i n i n g E t h y l e n e Diaminetetra-acetic acid (EDTA) as anticoagulant to prevent blood clothing before the analysis. Packed cell volume (PCV) and erythrocyte counts were determine as described by Ewuola and Egbunike, (2008) . Analysis to be obtained serum albumin, protein, urea, and creatinin were analyzed using sigma kits according to Feteris, (1965) total cholesterol was determined spectrophotometrically.
4
Evaluation of Liver and Kidney Function
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Liver functioning tests including ALT (alanine transferase) and AST (aspartate transferase) were determined by (DNPH-method) using Sigma Kits and ALP (alkaline phosphatase) was executed by adopting the alkaline phosphates (DGKC-method) (Basuny et al. 2009) . Kidney functioning tests comprising Serum urea (GLDH-method) and creatinine (Jaffe-method) values were documented to evaluate the renal functionality of variant experimental groups (Kumari et al. 2017) .
5
Avian Blood Biomarker Evaluation
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At the end of the experimental period (8 weeks), feed was withdrawn 12 hours prior to blood collection. Three birds were selected randomly from each replicate; the birds were bled using a sterilized disposable syringe and needle from punctured vein to aspirate 10 ml of blood samples from each bird. Five (5) ml of blood samples were collected into bottles treated with ethylene diamine tetra acetic acid (EDTA) for haematological assay. The remainder of each blood sample was allowed to coagulate to produce serum for blood chemistry measurements (Ochei and Kolhatkar, 2008) . (Lamb, 1991) . The mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC) were computed using the formulae outlined by Jain (1986) . Samples for biochemical parameters were first centrifuged and decanted. Sigma kits were thereafter used to determine protein, globulins and albumins. Serum aspartic aminotransferase (AST), alkaline phosphatase (ALP) and alkaline aminotransferase (ALT) were determined using the procedure of Baker and Silverton (1985) .
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