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2 methoxy polyethyleneoxy propyl trimethoxysilane peg silane

Manufactured by Gelest

2-[Methoxy(polyethyleneoxy)propyl]trimethoxysilane (PEG-silane) is a multifunctional organosilicon compound. It contains polyethylene glycol (PEG) and trimethoxysilane groups, which provide both hydrophilic and hydrophobic properties. The compound can be used in various applications that require surface modification and interfacial interactions.

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3 protocols using 2 methoxy polyethyleneoxy propyl trimethoxysilane peg silane

1

Silicon Wafer Mold Fabrication Protocol

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For silicon wafer mold fabrication, SU-8 2025 permanent epoxy negative photoresist and SU-8 developer were purchased from MicroChem (Newton, MA). Four-inch silicon wafers were purchased from University Wafer (Boston, MA). Custom laminated masks for patterning of photoresist were designed in AutoCad and sent for printing from CAD/Art Services, Inc. (Bandon, OR). Sylgard 184 silicone elastomer base and curing agent (Dow Corning, Midland, MI) were purchased from Krayden Inc. (Denver, CO). Extra dry acetone, sodium chloride, potassium chloride, sodium phosphate dibasic, potassium phosphate monobasic, Rhodamine B (RB), and Toluidine Blue (TB) were purchased from Sigma Aldrich (St. Louis, MO). Glass slides and cuvettes were purchased from Fisher Scientific. 2-[Methoxy(polyethyleneoxy)propyl]trimethoxysilane (PEG-silane) was purchased from Gelest (Morrisville, PA). Phosphate buffered saline (PBS), pH 7.4, containing 138 mM NaCl, 2.7 mM KCl, 10 mM sodium phosphate, and 100 mM EDTA was used for all experiments.
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2

Lipid Preparation and Characterization

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POPC, DOPE, POPG, E. coli Polar lipid extract (PLE) and TopFluor-CL were purchased from Avanti Polar Lipids. Lipids were stored at −20°C. Sodium chloride, hydrochloric acid, magnesium chloride, potassium chloride, glucose, glycerol, sucrose, malachite green hydrochloride, ammonium molybdate, tris(hydroxymethyl)aminomethane (Tris), dibasic potassium phosphate, adenosine 5'-triphosphate (ATP), bovine serum albumin (BSA), DL-dithiothreitol (DTT), n-Dodecyl-β-d-maltoside (DDM), methyl sulfonyl fluoride (PMSF), cOmpleteTM protease inhibitor cocktail, TOCL, 5-doxyl palmitoyl PC (5-doxyl PC) and 16-doxyl PC were purchased from Sigma Aldrich. 2-[Methoxy(polyethyleneoxy)propyl]-trimethoxysilane (PEG-silane) was purchased from Gelest. Lysogeny broth (LB) was purchased from Thermo Fisher. Phosphate buffered saline 6.7 mM (Ca2+ and Mg2+ free, pH 7) was purchasd from HyClone. Ultrapure water was produced from a Milli-Q unit (Millipore; conductivity lower than 18 MΩcm).
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3

Fluorescence Labeling of Biomolecules

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Tetraethyl orthosilicate (TEOS, 99.0%), fluorescein isothiocyanate (FITC), 3-aminopropyl-trimethoxysilane (APTMS, 97%), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N–lissamine rhodamine B sulfonyl ammonium salt (Rhodamine B PE) and fluorescein isothiocyanate labeled Ulex Europaeus Agglutinin (FITC-UEA1) were obtained from Sigma-Aldrich, USA. Other materials used include Silane-Polyethylene glycol-succinimidyl ester (Silane-PEG-NHS, MW 5000 Nanocs), 2-[Methoxy(polyethyleneoxy)propyl] trimethoxysilane, (PEG-silane, MW 596–725 g/mol, 9–12 EO, Gelest), Hexadecyltrimethylammoniumbromide (CTAB, 99%, Acrôs organics), Diacetylenic phosphatidylcholine 1,2-bis(tricosa-10,12-diynoil)-sn-glycero-3-phosphocholine (DAPC), N-glutaryl-L-α-phosphatidylethanolamine dimyristoyl (DMPC-GLU) (Avanti Polar Lipids, Alabaster, AL), Wheat Germ Agglutinin Alexa Fluor® 594 Conjugate (WGA594, Invitrogen), and 4',6-diamidino-2-phenylindole (DAPI, Invitrogen). Deionized water (18 MΩ) was used in all the experiments.
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