Antibodies against erk

Antibodies (Abs) and ELISA kits to measure IL-8, macrophage inflammatory protein 2 (MIP-2), monocyte chemoattractant protein-1 (MCP-1) and keratinocyte chemoattractant (KC) were purchased from R&D Systems (Minneapolis, MN, USA). Malondialdehyde (MDA) was purchased from Abcam (Cambridge, MA, USA). Antibodies against ERK, JNK, phospho-ERK, phospho-JNK, p65, and Histone H1 were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Mouse antibody against α-tubulin, EPA (purity ≥99%) and the 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) assay kit were purchased from Sigma-Aldrich (St. Louis, MO, USA). The EnzyChrom NADP⁺/NADPH assay kit was obtained from BioAssay Systems (Hayward, CA, USA). The membrane-permeable probes hydroethidine (HE) and dichlorofluorescein diacetate (DCFH-DA) were purchased from Molecular Probes (Eugene, OR, USA).

Antibodies (Abs) and ELISA kits to measure IL-8, macrophage inflammatory protein 2 (MIP-2), monocyte chemoattractant protein-1 (MCP-1), keratinocyte chemoattractant (KC), and interleukin-1β (IL-1β) were purchased from R&D Systems (Minneapolis, MN, USA). Rabbit antibody against 4-hydroxynonenal (4-HNE) was purchased from Abcam (Cambridge, MA, USA). Antibodies against ERK, JNK, p65, and Histone H1 were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Mouse antibody against α-tubulin, paeonol (purity > 99%, HPLC), N-acetyl-cysteine, and the 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) assay kit were purchased from Sigma-Aldrich (St. Louis, MO, USA). PD98059, SP600125, and BAY11-7085 were obtained from Calbiochem (San Diego, CA, USA). The membrane-permeable probe hydroethidine (HE) was purchased from Molecular Probes (Eugene, OR, USA).