LIQ (CAS No. 551-15-5; purity ≥ 98%), Ang II was sourced from Shanghai YuanYe Biotechnology (Shanghai, China). Primary antibodies including anti-TAK1, anti-phos-JNK1/2, and anti-JNK1/2 were purchased from CST (Boston, USA). Primary antibodies including anti-ATE1 and secondary antibodies were purchased from Abcam (Cambridge, England); anti-phos-TAK1 was purchased from Sabbiotech (Maryland, USA). CCK-8 was purchased from Beyotime Biotechnology (Shanghai, China). The Phalloidin Staining Kit was purchased from Solarbio (Beijing, China). The RT-qPCR kit, Trizol, and the SYBR Premix Ex Tap kit were purchased from Takara (Japan). Real-time PCR primers were obtained from Sangon Biotech (Shanghai, China). All other chemicals used were sourced from Thermo Fisher Scientific (Pittsburgh, PA, USA).
Ang 2
Manufactured by Yuanye Bio-Technology
Sourced in China
Ang II is a peptide hormone that plays a crucial role in the regulation of blood pressure and fluid homeostasis. It is a core component of the renin-angiotensin system.
Automatically generated - may contain errors
Lab products found in correlation
Rt qpcr kit, by Takara Bio (1 mentions)
Sybr premix ex tap kit, by Takara Bio (1 mentions)
Trizol, by Takara Bio (1 mentions)
Cck 8, by Beyotime (1 mentions)
Real time pcr primers, by Sangon (1 mentions)
F 12k medium, by American Type Culture Collection (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Procell (1 mentions)
Penicillin streptomycin, by Biosharp (1 mentions)
4 protocols using ang 2
1
Angiotensin II Signaling Pathway Protocol
2
Angiotensin II Effects on Axl Expression
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H9C2 cells were seeded (5×105 cells/well) into 12-well plates and cultured overnight. Cells were treated with different concentrations (0, 10−8, 10−7 or 10−6 M) of AngII (Shanghai YuanYe Biotechnology Co., Ltd.) for 6, 12 and 24 h at 37°C. Subsequently, reverse transcription-quantitative PCR (RT-qPCR) was performed to measure Axl expression levels.
3
Modeling Aortic-VSMC Transfection for TAD
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Human aortic-VSMCs (HA-VSMCs, ATCC, Manassas, VA, USA) were cultured in a complete medium composed of F-12K medium (ATCC) according to the manufacturer's recommendation. For mimic TAD cell models in vitro, HA-VSMCs were induced with 0.1 μM Ang II (Yuanye Bio-Technology, Shanghai, China) for 24 h. HA-VSMCs were transfected with circNRIP1 siRNAs (si-circNRIP1#1: 5′-GACAGACGGAAGTGTTTGGATdTdT-3′; si-circNRIP1#2: 5′-ACAGACGGAAGTGTTTGGATTdTdT-3′), CXCL5 siR-NAs (si-CXCL5#1: 5′-GAGAGAGCTGCGTTGCGTTTGT TTA-3′; si-CXCL5#2: 5′-GAGAGCTGCGTTGCGTTTGTTT ACA-3′), CXCL5 overexpression vector (oe-CXCL5), IGF2BP1 siRNA (si-IGF2BP1: 5′-CCGGGAAAGTAGAATTACAAGG AAA-3′) and their controls (si-NC and vector) (RiboBio, Guangzhou, China) by Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA).
4
Cardiomyocyte Hypertrophy Induction Protocol
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The H9c2 cardiomyocyte cell line was purchased from Procell Life Science & Technology Co., Ltd. Cells were maintained in high-glucose Dulbecco's modified Eagle's medium (DMEM; Procell Life Science & Technology Co., Ltd.) containing 10% fetal bovine serum (Gibco; Thermo Fisher Scientific, Inc.) and 1% penicillin-streptomycin (Biosharp Life Sciences) in a 5% CO2 incubator at 37°C. To induce the cardiomyocyte hypertrophy model, H9c2 cells were treated with either 60 µM ISO (MilliporeSigma) dissolved in phosphate-buffered saline (PBS) for 48 h, or 3 µM Ang II (Shanghai Yuanye Biotechnology Co., Ltd.) in PBS for 24 h in a 5% CO2 incubator at 37°C. The control group cells were treated with an equal volume of PBS in a 5% CO2 incubator at 37°C for 48 h (when compared with ISO) and 24 h (when compared with Ang II).
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