Aluminum starch octenylsuccinate (ASt) (DryFlo® Plus) was a gift from AkzoNobel (Amsterdam, The Netherlands). The oils used were liquid paraffin (LP) purchased from Mosselman (Ghlin, Belgium) and caprylic/capric acid triglyceride (Tegosoft® CT) (CT) a kind gift from Evonik Industries AG (Essen, Germany). Minocycline hydrochloride (MH) was obtained from Laboratórios Atral S.A. (Castanheira do Ribatejo, Portugal). Purified water was obtained by reverse osmosis and electrodeionization (Elix 3, Millipore, MA, USA) being afterwards filtered (pore 0.22 µm).
Elix 3
Manufactured by Merck Group
Sourced in United States, Germany, United Kingdom, Spain
The Elix 3 is a water purification system developed by the Merck Group. It is designed to produce high-quality purified water for use in various laboratory applications. The core function of the Elix 3 is to remove impurities from water, ensuring a consistent supply of purified water for research, analysis, and other laboratory processes.
Automatically generated - may contain errors
Lab products found in correlation
Milli q system, by Merck Group (14 mentions)
Formic acid, by Merck Group (14 mentions)
P coumaric acid, by Merck Group (4 mentions)
Caffeic acid, by Merck Group (4 mentions)
Vanillic acid, by Merck Group (4 mentions)
Epicatechin, by Merck Group (4 mentions)
Chromosolv for hplc, by ITW Reagents (4 mentions)
Acetonitrile, by Avantor (3 mentions)
Ethyl gallate, by Merck Group (3 mentions)
Gallic acid, by Merck Group (3 mentions)
Resveratrol, by Merck Group (3 mentions)
Ferulic acid, by Merck Group (3 mentions)
Apigenin, by Merck Group (3 mentions)
Synergy uv system, by Merck Group (3 mentions)
Quercetin, by Merck Group (3 mentions)
Rutin, by Merck Group (3 mentions)
Methanol, by ITW Reagents (3 mentions)
Hydrochloric acid (hcl), by Avantor (3 mentions)
Methanol, by Merck Group (3 mentions)
Methanol, by Honeywell (2 mentions)
51 protocols using elix 3
1
Formulation and Characterization of ASt-Based Topical Delivery
2
Optimized NdFeO3 Thin-Film Photocathodes
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Pristine NdFeO3 thin-film photocathodes were prepared
on fluorine-doped tin oxide conducting glass (TEC 15, 12–15
Ω/□, Pilkington) following a previously reported procedure
based on a citric acid-based sol–gel route.33 (link) The NdFeO3 precursor solution was prepared by
dissolving Nd(NO3)3·6H2O (Aldrich
Chemistry, 99%) 0.3 M and Fe(NO3)2·9H2O (Panreac, 98%) 0.3 M in water (Millipore, Elix 3). This
solution was magnetically stirred for 1 h and then the appropriate
amount of citric acid (Merck, 99.5%) was added to reach a concentration
of 0.6 M. The resulting solution was stirred for 20 h. Next, 30 μL·mL–1 acetylacetone (Fluka Analytical, 99.5%) and 30 μL·mL–1 Triton X-100 (laboratory grade, Sigma-Aldrich) was
added. An FTO glass plate (area to be covered: 1 cm2) was
cleaned by sonication (Selecta Ultrasonics) for 15 min in acetone
(VWR Chemicals, 99%) and ethanol (VWR Chemicals, 96%). It was then
spin-coated (Chemat Technology, KW-4A) at 1500 rpm for 20 s with 40
μL of the precursor solution. Finally, the electrodes were annealed
in air at 500 °C for 1 h using a programmable furnace (Conatec,
7800), with a heating rate of 5 °C·min–1. This process was repeated four times to obtain the optimized film
thickness (four layers, seeFigure S1 )
and a final thermal treatment at 640 °C was applied for 2 h to
obtain the crystalline perovskite films.
on fluorine-doped tin oxide conducting glass (TEC 15, 12–15
Ω/□, Pilkington) following a previously reported procedure
based on a citric acid-based sol–gel route.33 (link) The NdFeO3 precursor solution was prepared by
dissolving Nd(NO3)3·6H2O (Aldrich
Chemistry, 99%) 0.3 M and Fe(NO3)2·9H2O (Panreac, 98%) 0.3 M in water (Millipore, Elix 3). This
solution was magnetically stirred for 1 h and then the appropriate
amount of citric acid (Merck, 99.5%) was added to reach a concentration
of 0.6 M. The resulting solution was stirred for 20 h. Next, 30 μL·mL–1 acetylacetone (Fluka Analytical, 99.5%) and 30 μL·mL–1 Triton X-100 (laboratory grade, Sigma-Aldrich) was
added. An FTO glass plate (area to be covered: 1 cm2) was
cleaned by sonication (Selecta Ultrasonics) for 15 min in acetone
(VWR Chemicals, 99%) and ethanol (VWR Chemicals, 96%). It was then
spin-coated (Chemat Technology, KW-4A) at 1500 rpm for 20 s with 40
μL of the precursor solution. Finally, the electrodes were annealed
in air at 500 °C for 1 h using a programmable furnace (Conatec,
7800), with a heating rate of 5 °C·min–1. This process was repeated four times to obtain the optimized film
thickness (four layers, see
and a final thermal treatment at 640 °C was applied for 2 h to
obtain the crystalline perovskite films.
3
Melatonin and Formic Acid Analysis
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Melatonin and formic acid,
both of analytical grade, were obtained from Sigma-Aldrich, Germany.l -Tryptophan and serotonin were obtained from Fluka, Germany.
Acetic acid, methanol, and acetonitrile (ACN) were obtained from Riedel-de-Haen.
Ultrapure water was obtained using an Elix 3 (Millipore) system. All
of the solutions were protected from light and stored at 4 °C.
Syringe-driven filter units (0.2 μm) (Chromafil, PTFE, Macherey-Nagel)
were used for the filtration of all samples before HPLC analysis.
both of analytical grade, were obtained from Sigma-Aldrich, Germany.
Acetic acid, methanol, and acetonitrile (ACN) were obtained from Riedel-de-Haen.
Ultrapure water was obtained using an Elix 3 (Millipore) system. All
of the solutions were protected from light and stored at 4 °C.
Syringe-driven filter units (0.2 μm) (Chromafil, PTFE, Macherey-Nagel)
were used for the filtration of all samples before HPLC analysis.
4
Optimized In-line IMER-CZE-MS/MS Analysis
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Analytical grade reagents were used. Porcine pancreas trypsin (Type IX-S, lyophilized powder, Sigma, St. Louis, MO, USA) was used as a proteolytic enzyme. For in-solution digestions, trypsin was freshly prepared in double-deionized water; for IMER digestions, the enzyme was dissolved in acetic acid (pH = 4) solution. N-α-benzoyl-l -arginine ethyl ester hydrochloride (BAEE) substrate was used to examine the parameters affecting IMER efficiency. Human serum albumin (HSA) (Sigma) and human tears were digested to test the reliability of the in-line IMER in a CZE-MS/MS setup. BAEE, urea, dithiothreitol (DTT), iodoacetamide (IAM), NH4HCO3, and NH4Ac stock solutions (all Sigma products) were prepared in double-deionized water (Elix-3, Millipore, Darmstadt, Germany). HCl, NaOH, ammonium hydroxide, acetic acid (HAc) solutions, isopropanol, methanol, and acetonitrile were purchased from VWR (Radnor, PA, USA).
5
Melatonin Stock Solution Preparation
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Melatonin (Sigma, Steinheim, Germany, M5250) stock solution, 1 mg mL−1, was prepared in ethanol (Riedel-de Haen, Seelze, Germany), protected from light and stored at 4 °C. All other used reagents, MeCN (Riedel-de Haen), chloroform (Sigma, 366919), methanol (Riedel-de Haen,), NaCl (Sigma, S7653), MgSO4 (Sigma, M7506), sodium acetate (Sigma, S8750), and acetic acid (Sigma, A6283), were of analytical or chromatographic purity. The ultra-pure water was obtained with an Elix 3 (Millipore, Darmstad, Germany) system.
6
Phenolic Compounds Analytical Protocols
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All reagents employed, unless otherwise stated, were of analytical grade. Standards of arbutine, resveratrol, ethyl gallate, syringaldehyde, polydatin, tyrosol, umbelliferon, 4-hydroxybenzoic acid, caffeic acid, chlorogenic acid, gallic acid, homogentisic acid, p-coumaric acid, vanillic acid, ferulic acid, syringic acid, and trans-cinnamic acid were obtained from Sigma-Aldrich (Steinheim, Germany). The structures and CAS numbers of all studied phenolic compounds are summarized in Table 1 . Stock standard solutions of all phenolics (ca. 1000 mg/L) were prepared in methanol in amber glass vials. Intermediate working solutions were then prepared by appropriate dilution with Milli-Q water. All stock solutions were stored at 4 °C.
Methanol and acetonitrile (both UHPLC-gradient grade), absolute ethanol and acetone were purchased from Panreac (Barcelona, Spain). Water was purified using an Elix 3 coupled to a Milli-Q system (Millipore, Bedford, MA, USA) and filtered through a 0.22 µm nylon membrane integrated into the Milli-Q system.
Methanol and acetonitrile (both UHPLC-gradient grade), absolute ethanol and acetone were purchased from Panreac (Barcelona, Spain). Water was purified using an Elix 3 coupled to a Milli-Q system (Millipore, Bedford, MA, USA) and filtered through a 0.22 µm nylon membrane integrated into the Milli-Q system.
7
Diffusive and Ferrihydrite Gel DGT Sampling
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Diffusive (0.8 mm thick) and ferrihydrite resin gels (0.4 mm thick) were produced according to published procedures (Santner et al. 2010 (link); Zhang and Davison 1999 (link)). DGT samplers were obtained from DGT Research Ltd (Lancaster, UK). After DGT application, the samplers were retrieved and rinsed with lab water (18 MΩ cm, prepared by a Millipore Elix 3 water purification system). The samplers were then carefully opened, the ferrihydrite gel disk retrieved and eluted in 10 mL of 0.25 mol L−1 H2SO4. If low P recovery was expected, gels were eluted in 2 mL of 0.25 mol L−1 H2SO4. Ferrihydrite dissolves in 0.25 mol L−1 H2SO4, leading to a recovery of 100 % of the sorbed P (Santner et al. 2010 (link)).
8
Diffusive and Ferrihydrite Gel Preparation
9
Polyphenol Profiling in Sparkling Wines
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The mobile phase was prepared with formic acid (>96%, Sigma-Aldrich, St. Louis, MO, USA), methanol (UHPLC-Supergradient, Panreac, Barcelona, Spain) and water (Elix3, Millipore, Bedford, MA, USA). Polyphenols of analytical quality were purchased from Sigma-Aldrich (St. Louis, MO, USA): gallic, homogentisic, protocatechuic, caftaric, gentisic, vanillic, caffeic, syringic, ferulic, p- coumaric acids, (+)-catechin, (−)-epicatechin, ethyl gallate, resveratrol, rutin, myricetin and quercetin. These compounds were selected according to their abundance and relevance in white and rosé cavas. Stock standard solutions of 1000 mg L−1 of each polyphenol were prepared in MeOH. Working standard solutions consisting of a mixture of analytes at concentrations ranging from 20 to 0.05 mg L−1 were prepared in MeOH:water (1:1, v:v).
10
Gelatin-Based Biomaterial Preparation
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Type B gelatin powder was purchased from Acofarma (Madrid, Spain). Sucrose was obtained from Fisher Scientific (Hampton, USA). Glycerin was acquired to Lacrilar (Torres Vedras, Portugal). Cell culture medium (α-MEM), fetal bovine serum (FBS), and rhodamine B (RB, purity degree ≥ 95%) were obtained from Sigma-Aldrich (USA). Albumin bovine fraction V (BSA) MB grade was purchased from NZYTech (Lisboa, Portugal). Purified water was obtained by reverse osmosis and electrodeionization (Millipore®, Elix 3), followed by filtration (filter pore 0.22 µm) and sterilization.
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