Msu crystal

Manufactured by InvivoGen

Sourced in United States

MSU crystals are a type of laboratory equipment used for various research applications. They are composed of monosodium urate (MSU) and are designed to assist in the study of inflammatory responses and related biological processes. The core function of MSU crystals is to provide a controlled and consistent material for researchers to examine the formation, structure, and behavior of these crystalline structures in controlled experimental settings.

Automatically generated - may contain errors

Lab products found in correlation

Anti cd3 antibody, by BD (2 mentions) Ultrapure lps, by InvivoGen (2 mentions) Puno nlrp3, by InvivoGen (2 mentions) Z vad fmk, by InvivoGen (2 mentions) Adenosine triphosphate (atp), by InvivoGen (2 mentions) Mouse caspase 1, by R&D Systems (2 mentions) Ac yvad cmk, by InvivoGen (2 mentions) Human or murine il 1β elisa kits, by R&D Systems (2 mentions) Sytox green, by Thermo Fisher Scientific (2 mentions) Ouabain, by Merck Group (1 mentions) D2650, by Merck Group (1 mentions) Cgp084892, by Novartis (1 mentions) Afn700, by Novartis (1 mentions) Baa473, by Novartis (1 mentions) Salmonella typhimurium protein prgl, by Thermo Fisher Scientific (1 mentions) Mcc950, by Merck Group (1 mentions) Nigericin, by Enzo Life Sciences (1 mentions) Crt0066101, by Bio-Techne (1 mentions) Gramicidin, by Merck Group (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Monosodium urate (MSU) crystals (4 citations) Monosodium urate crystals (MSU) (2 citations)

18 protocols using msu crystal

Inflammatory Pathway Activation Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

All chemicals used in the present study were purchased from Sigma (St. Louis, MO), unless otherwise noted. Ultrapure LPS, Pm3C, ATP, MSU crystal, Caspase inhibitors Z-VAD-FMK, Ac-YVAD-cmk and puno-NLRP3 were purchased from Invivogen (San Diego, CA). Murine or human IL-1β, IL-10 from PeproTech (Rocky Hill, NJ); Anti-CD3 antibody from BD Biosciences (San Diego, CA); Human or murine IL-1β ELISA kits were from R&D system (Minneapolis, MN) or eBioscience (San Diego, California); Alum (Imject-alum) was purchased from Pierce Biochemicals. Antibodies to human IL-1β, human caspase 1, mouse IL-1β and mouse caspase 1 were from R&D Systems or Santa Cruz Biotechnology (Santa Cruz, CA).

Zhang J., Fu S., Sun S., Li Z, & Guo B. (2014). Inflammasome activation plays an important role in the development of spontaneous colitis. Mucosal immunology, 7(5), 1139-1150.

+ Open protocol

+ Expand

Inflammatory Pathway Activation Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

Zhang J., Fu S., Sun S., Li Z, & Guo B. (2014). Inflammasome activation plays an important role in the development of spontaneous colitis. Mucosal immunology, 7(5), 1139-1150.

+ Open protocol

+ Expand

Activation of Inflammasome Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols

Nigericin (Enzo, BML-CA421-005), ouabain (Sigma #O3125), gramicidin (Sigma #G5002), niclosamide (Adipogen #AG-CR1-3643), MSU crystals (InvivoGen # tlrl-msu), DMSO, used as vehicle for compound dilutions (Sigma #D2650), MCC950 (either from Sigma, #5381200001 or synthesized at Novartis), CGP084892 and AFN700 (both synthesized at Novartis), CRT0066101 (Tocris #4975). The pyrin activating compound BAA473 has been previously described [16 (link)]; it was synthesized at Novartis. The NLRC4 activating recombinant Salmonella Typhimurium Protein Prgl was from BioSource #MBS1177087.

Heiser D., Rubert J., Unterreiner A., Maurer C., Kamke M., Bodendorf U., Farady C.J., Roediger B, & Bornancin F. (2021). Evaluation of protein kinase D auto-phosphorylation as biomarker for NLRP3 inflammasome activation. PLoS ONE, 16(11), e0248668.

+ Open protocol

+ Expand

MSU Crystal-Induced Ankle Arthritis Model

Check if the same lab product or an alternative is used in the 5 most similar protocols

MSU crystals were purchased from Invivogen and verified to be endotoxin free by LAL assay from Pierce (Supplementary Figure 6). Using Hamilton syringes, 0.5 mg of MSU crystals (tlrl-msu) was injected into the tibio-tarsal joints of mice under light anesthesia according to a previously characterized model of MSU crystal-induced ankle arthritis (78 (link)). After 24 h, mice were anesthetized and ankle joints were photographed and measured via caliper. Ankle joint aspirates were collected by flushing with 20 μl of PBS and blood was taken for serum isolation by axillary vessel incision of the right subclavian vein at the time of sacrifice. Cytokine analysis on collected serum was performed using electrochemiluminescence detection (V-PLEX Proinflammatory Panel 1 mouse kit; Meso Scale Diagnostics, Rockville, MD) per manufacturer's protocol. Tibio-tarsal joints were isolated and cut in half. One half was for histological analysis and the other half was stored in TRIzol™ reagent (Life Technologies, 15596018) for RNA/protein analysis.

Caution K., Young N., Robledo-Avila F., Krause K., Abu Khweek A., Hamilton K., Badr A., Vaidya A., Daily K., Gosu H., Anne M.N., Eltobgy M., Dakhlallah D., Argwal S., Estfanous S., Zhang X., Partida-Sanchez S., Gavrilin M.A., Jarjour W.N, & Amer A.O. (2019). Caspase-11 Mediates Neutrophil Chemotaxis and Extracellular Trap Formation During Acute Gouty Arthritis Through Alteration of Cofilin Phosphorylation. Frontiers in Immunology, 10, 2519.

+ Open protocol

+ Expand

Chorionic Villi Explant Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols

Villous tissue was obtained from term uncomplicated pregnancies after caesarean section without labor (n=9) within 30 min after delivery and explants were prepared as previously described32 (link) with slight modifications. Briefly, biopsies of the chorionic villi were dissected and washed with PBS to remove any blood. Tissue was cut into small pieces (approx. 2-3mm) and three pieces (i.e. explants) were placed into netwells (74 μm mesh; Corning, Sigma-Aldrich, ON, Canada) in 1.5ml of culture media (RPMI, 5% heat-inactivated FBS, 100μg/ml streptomycin, 100IU/ml penicillin, 1μg/ml insulin, 0.1μg/ml hydrocortisone, 0.1μg/ml retinol acetate, 0.05mg/ml gentamycin; all chemical are from Sigma-Aldrich, ON, Canada). Explants were cultured at 37°C with 5% CO₂ with daily media change. On day 4, explants were treated with MSU crystals (100μg/ml; Invivogen, CA, USA), rhIL-1β (10ng/ml; Peprotech, Qc, Canada), caspase-1 inhibitor (10μM; Z-WEHD-FMK, R&D Systems, USA) or IL-1Ra (1μg/ml; Sobi-Swedish Orphan Biovitrum, ON, Canada) for 24 or 48h in Opti-MEM (Life Technologies, ON, Canada). Explants were processed for histology (fixed for 24h in 4% formalin and paraffin embedded) or protein analysis (supernatants and explants frozen and the stored at -80°C until extraction and analysis).

Brien M.E., Duval C., Palacios J., Boufaied I., Hudon-Thibeault A.A., Nadeau-Vallée M., Vaillancourt C., Sibley C.P., Abrahams V.M., Jones R.L, & Girard S. (2016). Uric acid crystals induces placental inflammation and alters trophoblast function via an IL-1-dependent pathway: implication for FGR. Journal of immunology (Baltimore, Md. : 1950), 198(1), 443-451.

+ Open protocol

+ Expand

Neutrophil Isolation and Stimulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human blood was collected into EDTA tubes, and neutrophils were purified using the EasySep Direct Neutrophil Isolation Kit (StemCell Technologies, Vancouver, Canada) according to the manufacturer's protocol. Neutrophil purity was at least 95% by flow cytometry. Neutrophils were plated onto acid-washed, poly-L-lysine (Sigma Diagnostics, Livonia, USA) coated 12 mm glass coverslips at a concentration of 50,000 cells per coverslip in media containing RPMI 1640 (Thermo Fisher Scientific, Waltham, USA) with 2% fetal bovine serum (Atlanta Biologicals, Flowery Branch, USA) and 1% penicillin-streptomycin solution (Corning, Tewksbury, USA). Neutrophils were treated with the following and incubated for 4 hours at 37°C, 5% CO₂: 4 μM ionomycin (MilliporeSigma, Darmstadt, Germany), 560 μg/mL MSU crystals (InvivoGen, San Diego, USA), 25 nM PMA (Fisher BioReagents, Waltham, USA), or 1 × 10⁶Candida albicans strain SC5314 [42 (link)].

Holmes C.L., Shim D., Kernien J., Johnson C.J., Nett J.E, & Shelef M.A. (2019). Insight into Neutrophil Extracellular Traps through Systematic Evaluation of Citrullination and Peptidylarginine Deiminases. Journal of Immunology Research, 2019, 2160192.

+ Open protocol

+ Expand

Antibody-based Investigation of Neutrophil Extracellular Traps

Check if the same lab product or an alternative is used in the 5 most similar protocols

The antibodies used were as follows: for western blotting and immunohistochemistry, rabbit monoclonal anti-myeloperoxidase (MPO) antibody (Abcam, Cambridge, UK), rabbit monoclonal anti-citrullinated histone H3 (H3cit) antibody (Abcam, Cambridge, UK), rabbit monoclonal anti-p53 antibody (Abcam, Cambridge, UK), rabbit monoclonal anti-ATG7 antibody (Abcam, Cambridge, UK), mouse monoclonal anti-PAD4 antibody (Abcam, Cambridge, UK) and rabbit monoclonal anti-GAPDH antibody (Cell Signaling Technology, MA, USA) were used. For immunofluorescence, mouse monoclonal anti-MPO antibody (Abcam, Cambridge, UK), rabbit monoclonal anti-H3cit antibody (Abcam, Cambridge, UK), Rhodamine Red-X (RRX) goat anti-mouse IgG (H+L) and FITC-AffiniPure goat anti-rabbit IgG (H+L) (Jackson, PA, USA) were used. The following chemicals were also utilized: MSU crystals (In vivoGen, CA, USA); SYTOX Green (Thermo Fisher Scientific, MA, USA); Rapamycin (RAPA) and 3-Methyladenine (3MA) (Sigma-Aldrich, MO, USA); Pifithrin-α (Sigma-Aldrich, MO, USA). The recombinant human proteins used included recombinant p53 and recombinant ATG7 (R& D system, MN, USA).

Huang S., Wang Y., Lin S., Guan W., Liang H, & Shen J. (2023). Neutrophil autophagy induced by monosodium urate crystals facilitates neutrophil extracellular traps formation and inflammation remission in gouty arthritis. Frontiers in Endocrinology, 14, 1071630.

+ Open protocol

+ Expand

Peritoneal Inflammation Induction

Check if the same lab product or an alternative is used in the 5 most similar protocols

Peritonitis was induced by injection of MSU crystals i.p. (Invivogen). Mice were injected with 2.5mg MSU in PBS. Four hours later, total peritoneal cells were collected by lavage. Cells were counted by hemacytometer and phenotype and gene expression were analyzed by flow cytometry and RT-PCR, respectively.

Goldberg E.L., Asher J.L., Molony R.D., Shaw A.C., Zeiss C.J., Wang C., Morozova-Roche L.A., Herzog R.I., Iwasaki A, & Dixit V.D. (2017). β-hydroxybutyrate deactivates neutrophil NLRP3 inflammasome to relieve gout flares. Cell reports, 18(9), 2077-2087.

+ Open protocol

+ Expand

MSU Crystals Induce Inflammatory Responses

Check if the same lab product or an alternative is used in the 5 most similar protocols

MSU crystals were purchased from InvivoGen (San Diego, CA, USA) and used freshly for in-vitro treatment by dissolving in phosphate buffered saline (PBS). Antibodies for cleaved caspase-1 (p20), phospho-NF-κB p65 (ser536), and β-actin were purchased from Cell Signaling Technology (Danvers, MA, USA). Human and mouse mature IL-1β were measured using the IL-1β ELISA kit (Neobioscience, Shenzhen, China). PerCP-CD45, PE-F4/80, and APC-Gr-1 antibodies were obtained from BioLegend (San Diego, CA, USA).

Ma T., Liu X., Cen Z., Xin C., Guo M., Zou C., Song W., Xie R., Wang K., Zhou H., Zhang J., Wang Z., Bian C., Cui K., Li J., Wei Y.Q., Li J, & Zhou X. (2018). MicroRNA-302b negatively regulates IL-1β production in response to MSU crystals by targeting IRAK4 and EphA2. Arthritis Research & Therapy, 20, 34.

+ Open protocol

+ Expand

Macrophage Differentiation and Stimulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Whole bone marrow was obtained from murine long bones, hips, and spine. Bone marrow–derived macrophages (BMDM) were grown in Iscove's Modified Dulbecco's Medium with 10% fetal bovine serum, 1% penicillin/streptomycin/glutamine, and 10 ng/mL of murine macrophage colony-stimulating factor from animals with the following genotypes: wild-type (WT), Tet2 KO, Nlrp3 KO, Nlrp3 KO/Tet2 KO, and Dnmt3a KO/Nlrp3 KO. Macrophages were differentiated for 10 to 14 days and harvested thereafter using a cell scraper. BMDM were then replated into 24-well plates with 400 000 cells/well. After 24 hours, cells were stimulated with lipopolysaccharide (Invivogen) 1 ng/mL for 3 hours followed by administration of MSU crystals (Invivogen) for 6 hours. Controls received either PBS only or lipopolysaccharide followed by PBS.

Agrawal M., Niroula A., Cunin P., McConkey M., Shkolnik V., Kim P.G., Wong W.J., Weeks L.D., Lin A.E., Miller P.G., Gibson C.J., Sekar A., Schaefer I.M., Neuberg D., Stone R.M., Bick A.G., Uddin M.M., Griffin G.K., Jaiswal S., Natarajan P., Nigrovic P.A., Rao D.A, & Ebert B.L. (2022). TET2-mutant clonal hematopoiesis and risk of gout. Blood, 140(10), 1094-1103.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!