Plain radiological images of the femora were obtained at seven, 14, and 21 days post-surgery. Image analysis was performed using the common format (DICOM). Micro-CT was performed with a micro-CT scanner (Skyscan 1176; Bruker, Belgium) set at a 9 µm voxel size. The images were processed and reconstructed using the CTVol software (Bruker), as previously described. The trabecular bone volume fraction (BV/TV), relative callus volume, trabecular spacing (Tb.Sp), and bone mineral density (BMD) were measured from the reconstructed images.
Ctvol software
Manufactured by Bruker
Sourced in Belgium, United States, Germany
CTVol software is a computer program developed by Bruker to visualize and analyze three-dimensional (3D) data obtained from various imaging techniques, such as X-ray computed tomography (CT) and micro-CT. The software's core function is to provide users with tools to process, manipulate, and interpret 3D data sets.
Automatically generated - may contain errors
Lab products found in correlation
Ctan software, by Bruker (26 mentions)
Nrecon software, by Bruker (17 mentions)
Skyscan 1176, by Bruker (6 mentions)
Skyscan 1172, by Bruker (6 mentions)
Dataviewer software, by Bruker (5 mentions)
Skyscan 1172 system, by Bruker (4 mentions)
Skyscan 1275, by Bruker (4 mentions)
Skyscan 1276, by Bruker (4 mentions)
Dataviewer, by Bruker (3 mentions)
Skyscan 1276 system, by Bruker (2 mentions)
Skyscan 1076, by Bruker (2 mentions)
Skyscan 1174, by Bruker (1 mentions)
Ct40 scanner, by Scanco (1 mentions)
Skyscan 1173 micro ct, by Bruker (1 mentions)
Nrecon reconstruction software, by Bruker (1 mentions)
Skyscan software, by Bruker (1 mentions)
Ct analyzer software, by Bruker (1 mentions)
Skyscan 1176 micro ct, by Bruker (1 mentions)
Amira avizo software, by Thermo Fisher Scientific (1 mentions)
Microfil injection compound, by Flow Tech (1 mentions)
45 protocols using ctvol software
1
Radiological and Micro-CT Analysis of Femoral Callus
2
Quantitative Femoral Bone Microstructure
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Micro-computed tomography (μCT) was performed on distal femurs with the Skyscan 1172 system (Bruker), as described previously.19 (link) 3D models of bones were generated using CTVol software (Bruker). The number of cortical perforations > 50 μm in diameter was counted blinded on reconstructed images.
3
Spinal Fusion Histomorphometric Analysis
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Spines were subjected to tomographic scanning using a Skyscan 1174 high resolution specimen imager (Bruker, Kontich, Belgium). Images were scanned at 26 W, 661 μA, 39 kV with 33 μm/pixel resolution. Axial images were generated using NRecon software (Bruker, Billerica, MA), with thresholding set to 500–10,000 Hounsfield Units. In most cases, it was impossible to distinguish the new bone from the mature transverse processes because outgrowths were completely contiguous with original bone. We therefore used previously described methods that compared total volumes of fused vertebrae 41 . For histomorphometric analysis of fusion beds, specimens were scanned at 18 μm/pixel resolution with image smoothing and ring artifact compensation set to maximum. To maximize resolution, 10 images were averaged per degree for 360°. Axial images were generated with thresholding set to 300–3,200 Hounsfield Units. A region of interest consisting of one hundred axial images taken from the left fusion bed, in line with the central 1.8 mm of the L5 vertebra, was selected and subjected to a comprehensive 3D histomorphometric analysis. Values from the BIO‐treated and control groups were analyzed statistically by Student's t test and p‐values <.05 were considered statistically significant. Three‐dimensional images were rendered by CTvol software (Bruker).
4
Quantifying Bone Regeneration in Cranial Defects
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The animals were sacrificed 28 days after surgery and the cranial region was imaged using SkyScan1176 in vivo microcomputed tomography (μCT; Bruker, Billerica, MA). Three-dimensional reconstructions were generated using CTVOL software (Bruker). The volume of newly formed bone inside the bone defect was determined using CT-An software (Bruker).
5
Quantitative Micro-CT Analysis of Knee Joints
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Quantitative analysis of hind knee joints was performed using a micro-CT system (Skyscan 1172, Bruker, Kontich, Belgium). Specimens were scanned using micro-CT with an X-ray source of 40 kV/250 μV, pixel size 23 μm, and a 0.5 mm aluminum filter. After scanning, cross-sectional slices were reconstructed and each scan result was reconstructed using the 0–0.14 threshold values to distinguish bone from air.
Three-dimensional analysis was performed using CTAn software (Bruker). The fraction of bone volume, percent bone volume, trabecular number, trabecular thickness, bone surface/bone volume and trabecular separation were performed using the built-in software. In addition, osteophytes within each contiguous coronal image section were manually outlined and their volumes were calculated using CTvol software (Bruker).
Three-dimensional analysis was performed using CTAn software (Bruker). The fraction of bone volume, percent bone volume, trabecular number, trabecular thickness, bone surface/bone volume and trabecular separation were performed using the built-in software. In addition, osteophytes within each contiguous coronal image section were manually outlined and their volumes were calculated using CTvol software (Bruker).
6
Micro-CT Analysis of Trabecular Bone
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For the analysis of trabecular bone microstructure, we employed the micro-CT SkyScan 1276 system (Bruker, Kontich, Belgium) to perform scans on the distal femoral bone. The reconstruction of the scanned images was carried out using the NRecon software (version 1.7.42, Bruker). Three-dimensional images of the distal femoral bone were generated using CTvol software (version 2.3.2.0, Bruker). The volume of interest for trabecular bone analysis in the distal femur spanned from 50 µm below the growth plate to the proximal direction, covering a total length of 1.0 mm. Bone mineral density (BMD) and bone morphometric parameters were analyzed using CTAn software (version 1.20.3.0, Bruker).
7
Micro-CT Analysis of Implanted Scaffolds
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Pre and post implanted scaffolds were wrapped in a transparent plastic film and mounted for scanning using submicron resolution μCT (Skyscan 1176, Skyscan, Belgium) and scan was performed with the settings: 45 kV X-ray voltage, 0.2 mm aluminium filter, source current 556 μA, 0.5° rotation step, exposure time of 180 ms for each section. Three-dimensional (3D) models were reconstructed using Skyscan NRecon software (Bruker micro-CT, Belgium) and reconstructed dataset was processed after segmentation and binarisation to analyse pore size, pore thickness, porosity % by CTAn software (Bruker micro-CT, Belgium), angiogenesis by CTVol software (Bruker micro-CT, Belgium) and volume degradation by Drishti software (ANU, Australia).
8
Femur Micro-CT Imaging and Analysis
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The femur was imaged by micro-CT using a three-dimensional microfocus computed tomography instrument (Sky-Scan 1176; Sky-Scan, Kontich, Belgium). The sample was wrapped in plastic wrap to prevent drying. X-ray alignment was performed by the software alignment system; the program setting was 2000 × 1336 (medium), the camera focus was set to 18 μm, and an aluminum filter of 1 mm was used. Images were reconstructed using NRecon software (ver. 1.6.9.4; Bruker, Billerica, MA, USA), and coronal, sagittal, and transverse bone structures were evaluated using DataViewer software (ver. 1.5.1.2; Bruker). Three-dimensional analysis was performed using CTAn software (ver. 1.13.5.1+; Bruker), and three-dimensional rendering was using CTvol software (ver. 2.2.3.0; Bruker). The femur was scanned using CT-analyzer™ software (SkyScan). All samples were analyzed with the same anatomical structure, and a thickness of 2 mm was set at a distance of 2 mm from the growth plate. The percentage bone volume to total volume (BV/TV) of the samples was calculated.
9
Quantitative Analysis of Hind Knee Joint
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Quantitative analysis of the hind knee joints was performed using a micro-CT system (SKYSCAN 1172; Bruker, Belgium). Specimens were scanned with an X-ray source of 40 kV/250 µV, pixel size of 23 µm, and a 0.5 mm aluminum filter. After scanning, cross-sectional slices were generated and each scan result was reconstructed using 0~0.14 threshold values to distinguish bone from air.
Three-dimensional analysis was performed using CTAn software (Bruker). The fraction of bone volume (BV), percent bone volume (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th), bone surface/bone volume (BS/BV), and trabecular separation (Tb.Sp) were evaluated at weeks 0, 2, 3, and 4 using the built-in software. In addition, osteophytes within each contiguous coronal image section were manually outlined and the volume was calculated using CTvol software (Bruker).
Three-dimensional analysis was performed using CTAn software (Bruker). The fraction of bone volume (BV), percent bone volume (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th), bone surface/bone volume (BS/BV), and trabecular separation (Tb.Sp) were evaluated at weeks 0, 2, 3, and 4 using the built-in software. In addition, osteophytes within each contiguous coronal image section were manually outlined and the volume was calculated using CTvol software (Bruker).
10
Femur Trabecular Bone Microstructure Analysis
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After the mice were euthanized by cervical dislocation, femurs were harvested and fixed in 4% PFA for 48 h. Subsequent µCT analysis was conducted to evaluate parameters of the trabecular bone in the distal metaphysis of the femurs using a Scanco µCT-40 scanner (Scanco Medical, Bassersdorf, Switzerland). Images were acquired at 55 kV and 145 mA with an isotropic voxel size of 6 μm, and 300 slices (1.8 mm) proximal to the growth plate were selected as the regions of interest (ROIs) for analysis. Bone parameters, including bone volume fraction (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th), and trabecular separation (Tb.Sp) were analyzed using NRecon software (version 1.6, Bruker), CTAn software (version 1.9, Bruker) and CTVol software (version 2.0, Bruker).
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