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αcd56 clone ncam16

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αCD56/clone NCAM16.2 is a monoclonal antibody that recognizes the NCAM (Neural Cell Adhesion Molecule) protein, also known as CD56. It is commonly used as a marker for natural killer (NK) cells and certain types of neural and neuroendocrine cells.

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Lab products found in correlation

αcd103 clone 2e7, by BioLegend (2 mentions) αcd14 clone 61d3, by BD (2 mentions) αcd3 clone sk7, by BD (2 mentions) Rnaprotect reagent, by Qiagen (2 mentions) Collagenase 4, by Merck Group (2 mentions)

Close spelling variants of this product

Clone NCAM16.2 NCAM16.2

2 protocols using αcd56 clone ncam16

1

Isolation and Analysis of Splenic Phagocytes

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Splenic phagocyte cell populations (Mϕ and DC) were sorted to assess cellular source of AhR activation and cytokine responses to apoptotic cells. Mice received 2X107 apoptotic cells i/v and 8h later harvested spleens were injected with 100U/ml of Collagenase IV (Sigma) in 2mL RPMI (supplemented with 10% FBS) and further incubated for 30 min at 37°C in 5 mL RPMI containing 400 U/mL of Collagenase IV (Sigma). From the digest single-cell suspensions were generated and incubated with αSignR1 (clone ERTR9), αCD169 (clone MOMA-1) (both from Serotec), α-CD8α (clone 53–6.7), α-CD11c (clone N418), α-F4/80 (clone BM8), and αCD103 (clone 2e7) (all from Biolegend).
For sorts from human samples, PBMC fractions were divided into 2 groups: for monocytic cell enrichment the PBMCs were stained with APC-labelled lineage markers- (αCD56/clone NCAM16.2, αCD19/clone HIB19, and αCD3/clone UCHT1) to remove T cells, B cells, and NK cells and markers for DC, pDC, and monocytes- αCD11c (clone B-Ly6), αCD123 (clone 7G3), αCD11b (clone ICRF44), αHLA-DR (clone G46-6), αBDCA-2/CD303 (clone V24-785), and αCD14 (clone 61D3) (all from BD Biosciences). For T cell enrichment the PBMC fraction was stained with αCD4 (clone RPA-T4), αCD8 (clone SK1), and αCD3 (clone SK7) (all from BD Biosciences). All cells were sorted on a Dako Cytomation MoFlo cell sorter into tubes containing RNA protect reagent (Qiagen).
Shinde R., Hezaveh K., Halaby M.J., Kloetgen A., Chakravarthy A., da Silva Medina T., Deol R., Manion K.P., Baglaenko Y., Eldh M., Lamorte S., Wallace D., Chodisetti S.B., Ravishankar B., Liu H., Chaudhary K., Munn D.H., Tsirigos A., Madaio M., Gabrielsson S., Touma Z., Wither J., De Carvalho D.D, & McGaha T.L. (2018). Apoptotic cell–induced, TLR9-dependent AhR activity is required for immunological tolerance and suppression of systemic lupus erythematosus in mice and humans. Nature immunology, 19(6), 571-582.
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2

Isolation and Analysis of Splenic Phagocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Splenic phagocyte cell populations (Mϕ and DC) were sorted to assess cellular source of AhR activation and cytokine responses to apoptotic cells. Mice received 2X107 apoptotic cells i/v and 8h later harvested spleens were injected with 100U/ml of Collagenase IV (Sigma) in 2mL RPMI (supplemented with 10% FBS) and further incubated for 30 min at 37°C in 5 mL RPMI containing 400 U/mL of Collagenase IV (Sigma). From the digest single-cell suspensions were generated and incubated with αSignR1 (clone ERTR9), αCD169 (clone MOMA-1) (both from Serotec), α-CD8α (clone 53–6.7), α-CD11c (clone N418), α-F4/80 (clone BM8), and αCD103 (clone 2e7) (all from Biolegend).
For sorts from human samples, PBMC fractions were divided into 2 groups: for monocytic cell enrichment the PBMCs were stained with APC-labelled lineage markers- (αCD56/clone NCAM16.2, αCD19/clone HIB19, and αCD3/clone UCHT1) to remove T cells, B cells, and NK cells and markers for DC, pDC, and monocytes- αCD11c (clone B-Ly6), αCD123 (clone 7G3), αCD11b (clone ICRF44), αHLA-DR (clone G46-6), αBDCA-2/CD303 (clone V24-785), and αCD14 (clone 61D3) (all from BD Biosciences). For T cell enrichment the PBMC fraction was stained with αCD4 (clone RPA-T4), αCD8 (clone SK1), and αCD3 (clone SK7) (all from BD Biosciences). All cells were sorted on a Dako Cytomation MoFlo cell sorter into tubes containing RNA protect reagent (Qiagen).
Shinde R., Hezaveh K., Halaby M.J., Kloetgen A., Chakravarthy A., da Silva Medina T., Deol R., Manion K.P., Baglaenko Y., Eldh M., Lamorte S., Wallace D., Chodisetti S.B., Ravishankar B., Liu H., Chaudhary K., Munn D.H., Tsirigos A., Madaio M., Gabrielsson S., Touma Z., Wither J., De Carvalho D.D, & McGaha T.L. (2018). Apoptotic cell–induced, TLR9-dependent AhR activity is required for immunological tolerance and suppression of systemic lupus erythematosus in mice and humans. Nature immunology, 19(6), 571-582.
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