Bp 2010a

Blood pressure measurements were performed from 8:00 a.m. to 6:00 p.m. in a warm and quiet room once a week. A rat was selected randomly for BP measurement from group A, followed by B, C, D, and E, sequentially. After one round, repeat the abovementioned operations until all BP measurements are completed. Systolic blood pressure and diastolic blood pressure (DBP) of conscious rats were measured by the tail-cuff system (BP-2010A, Softron, China) as described previously (19 (link)). Five BP measurements were recorded consecutively for each rat. If the difference between any two of the five BP readings per individual rat exceeded 10 mmHg, a sixth BP measurement was conducted. The mean value was calculated for analysis. Hypertension was defined as a SBP ≥ 140 mmHg.

129 wild-type (WT) male mice of 8 weeks old were purchased from Department of Laboratory Animal Science of Peking University (Beijing, China) and SIRT3-knockout (SIRT3-KO) mice were purchased from the Jackson Laboratories (USA). SIRT3-overexpression (SIRT3-LV) mice were obtained by injection to the 129 WT mice SIRT3-overexpression-lentivirus (JIKAI GENE, Shanghai, China) via caudal vein. HKL were intraperitoneally injected in 129 WT mice for 42 days (25 mg/Kg, once a day) as the HKL treatment group. All experimental mice anesthetized with isoflurane (1%) were implanted subcutaneously corresponding osmotic pumps beforehand filled with AngII or saline for 42 days. The osmotic pumps infused with appropriate doses of AngII in sterile saline (2000 ng/kg per min) or saline according to the directions were placed in sterile 0.9% saline at 37°C for 60 hours to prime. Together, the experimental animals were randomly assigned to eight groups: WT+saline group, WT+AngII group, SIRT3-KO+saline group, SIRT3-KO+AngII group, SIRT3-LV+saline group, SIRT3-LV+AngII group, WT+HKL+saline group, WT+HKL+AngII group. Mice were sacrificed at the end of the experiment and the kidneys were used to assay for renal fibrosis. In addition, Blood pressure were measured by Tail-Cuff way (BP2010A, softron, Japan) before modeling and death.