Gc8860 ms5977b

HPLC (High performance liquid chromatography, 1260 Infinity II, Agilent) equipped with a Raptor ARC-18 for LC-UV column (150 mm × 4.6 mm ID, pore size 2.7 µm) was used to analyze phytocannabinoids content in each fraction as described previously (Peeri et al., 2021 (link); Shalev et al., 2022 (link)). For chemical analysis of terpenes, 1 μL of each sample was analyzed by a gas chromatography-mass spectrometer (GC8860-MS5977B Agilent) equipped with 30 m, 0.25 mm ID, 5% cross-linked phenylmethyl siloxane capillary column (HP-5MS) with 0.25-μm film thickness, was used as described in (Peeri et al., 2021 (link)).

Chemical analysis was done, as described previously [19 (link),20 (link)]. Briefly, high performance liquid chromatography (HPLC, 1260 Infinity II, Agilent), equipped with a Raptor ARC-18 for LC-UV column (150 mm × 4.6 mm ID, pore size 2.7 µm), was used for chemical analysis. A gas chromatography-mass spectrometer (GC/MS; GC8860-MS5977B Agilent), equipped with a 30 m, 0.25 mm ID, 5% cross-linked phenylmethyl siloxane capillary column (HP-5MS) with 0.25-μm film thickness, was used for chemical analysis, as described in [20 (link)]. An amount of 10 μL of each sample fraction was transferred into GC vials with an insert, dried under a gentle stream of nitrogen and dissolved in 100 μL of hexane. Sample volume for injection was 1 μL. Helium was used as the carrier gas at a constant flow of 1.1 mL s⁻¹. An isothermal hold at 50 °C was maintained for 2 min, followed by a heating gradient of 6 °C min⁻¹ to 300 °C, with the final temperature held for 4 min. Peaks were assigned using spectral libraries (NIST 14.0 and 17.0) and compared with MS data obtained from the injection of standards purchased from LGC Standards.