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Nicolet ftir spectrometer

Manufactured by Thermo Fisher Scientific
Sourced in United States

The Nicolet FTIR spectrometer is a laboratory instrument that utilizes Fourier Transform Infrared (FTIR) technology to analyze the composition of materials. It measures the absorption or transmission of infrared light by a sample, providing detailed information about its molecular structure and chemical properties. The spectrometer features a high-resolution optical system and advanced data processing capabilities.

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22 protocols using nicolet ftir spectrometer

1

Synthesis and Characterization of Sorbate Ionic Liquids

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Sorbate ILs (SCILs) were synthesized by a salt metathesis reaction. In brief, sorbic acid and choline bicarbonate (molar ratio of 1:1) were weighed separately. Then, sorbic acid was dissolved in an appropriate amount of methanol. Choline bicarbonate was put into a 100 ml round‐bottom flask. The methanol solution of sorbic acid was added dropwise into choline bicarbonate. Reaction was kept under magnetic stirring until no bubbles overflow. Next, solvents of the resultant mixture were removed by rotary evaporation at 60°C for 30 min. Finally, the synthesized SCILs were placed in a vacuum oven and dried for 48 h to get rid of residual solvents.
The SCILs were characterized by 1H‐nuclear magnetic resonance (1H‐NMR) in DMSO‐d6 with the residual solvent signals as the internal standard on a Bruker Ascend™ 600 MHz spectrometer (BRUKER AXS GmbH, Berne, Switzerland). The FTIR and differential scanning calorimetry (DSC) spectra of SCILs were recorded using a Nicolet FTIR spectrometer (Thermo Fisher Scientific Inc) and a 214 Polyma differential scanning calorimeter (NETZSCH Gerätebau GmbH, Selb, Germany), respectively.
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2

FTIR Analysis of Chitin and Chitosan from Tenebrio Molitor

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Using a Thermo Nicolet FTIR Spectrometer, extracted chitin and chitosan samples from Tenebrio Molitor beetles were examined at 4000 to 400/cm. The following formula was used to compute the degree of acetylation (DA) of chitin and the degree of deacetylation (DD) of chitosan[15 ]:
DA (%) = (A1655/A3450) × 100
DD (%) = 100 -[(A1658/A3450) × 115]
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3

FT-IR Spectroscopy of Material Samples

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Samples were characterized by Fourier transform infrared spectroscopy (FT-IR). A Nicolet FT-IR spectrometer (Thermo Fisher Scientific, Madison, WI, USA) was employed coupled with the Omnic® v6.1 software (Thermo Nicolet Corporation, Waltham, MA, USA). Spectral data were collected at room temperature and accumulated from 16 scans with a resolution of 4 cm−1 in the range of 400 to 4000 cm−1. A correction was applied by subtracting the background spectrum of air. KBr pellets were prepared by grinding with ~5-mg sample on an agate mortar followed by compression at ~10,000 psi for 2 min.
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4

Deuterium-Labeled Thylakoid Membranes FTIR Analysis

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Thylakoid membranes isolated from A. thaliana accessions were resuspended in a D2O-based 20 mM Hepes–NaOH (pH 7.0) buffer containing 330 mM sorbitol, 15 mM NaCl, and 4 mM MgCl2 (Merck KGaA, Darmstadt, Germany) and then centrifuged at 7200× g for 10 min at 4 °C (Beckman Coulter, Brea, CA, USA). This step was repeated three times to replace the H2O-based buffer with a D2O one (Sigma Aldrich Inc., Saint Louis, MO, USA). Fourier-transform infrared (FTIR) spectra were recorded on a Nicolet FTIR Spectrometer (Thermo Scientific, Waltham, MA, USA). Sample measurements and data analysis were performed as described previously [56 (link)].
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5

NIR Spectroscopy of Clay-Sized Minerals

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Near-infrared spectra of the clay-sized separates were stored at room temperature and then analyzed in the lab using the diffuse reflection method126 (link) (DRIFT) with a Nicolet FTIR spectrometer (Thermo Fisher Scientific, Massachusetts, USA). Samples were poured into a sample cup without dilution in KBr. Analysis were done at room temperature and under air-dried atmosphere. We used a mirror for background measurement. Spectra were collected using a DTGS-KBr detector at 2 cm−1 resolution in the NIR region (from 12,000 to 4000 cm−1; 1 to 2.5 μm) with a quartz beamsplitter.
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6

Characterization of Crystalline Samples

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Example 3

XRD scans were obtained using a Rigaku 18 kW rotating anode generator and a high resolution powder diffractometer. The diffraction scans were collected using monochromatic CuKα radiation.

Raman spectra were collected on an in Via Raman Microscope (Renishaw) using a 514 nm laser line.

Scans were obtained at ca. 15 mW laser power at the sample and an integration period of 30 seconds.

Fourier Transform Infrared (FTIR) spectra were collected using Thermo Scientific Nicolet FT-IR spectrometer with Diffuse Reflectance Infrared Transform Spectroscopy (DRIFTS) accessory.

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7

FTIR Analysis of Liposome-SA Interactions

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The interactions between the liposome and the SA were analyzed by Fourier transform infrared spectroscopy using a Nicolet FTIR spectrometer (Thermo Fisher Scientific, Waltham, MA, USA). The samples were ground carefully with KBr and then pressed into disks for observation, and the scanning range was 4000 cm−1 to 500 cm−1.
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8

FT-IR Spectral Analysis of FSE and β-CD

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FSE, β-CD, FSE-β-CD, FSE and β-CD 1:1 physical mixture (1:1 FSE/β-CD PM) were fully mixed with an appropriate amount of KBr and then compressed into KBr disks, respectively. Then, the FT-IR spectrum was obtained using a Nicolet FT-IR spectrometer (Thermo Fisher Scientific, Waltham, MA, USA). Each sample was scanned 64 times between 400 and 4000 cm−1 with an optical resolution of 4 cm−1.
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9

FTIR Analysis of Treated Jute Fibers

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The chemical structures and compositions of treated and untreated jute fibers were analyzed by a Thermo Nicolet FTIR spectrometer. The jute fibers were mixed up with KBr and tableted.
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10

Comprehensive Material Characterization Protocol

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Example 3

XRD scans were obtained using a Rigaku 18 kW rotating anode generator and a high resolution powder diffractometer. The diffraction scans were collected using monochromatic CuKα radiation.

Raman spectra were collected on an in Via Raman Microscope (Renishaw) using a 514 nm laser line.

Scans were obtained at ca. 15 mW laser power at the sample and an integration period of 30 seconds.

Fourier Transform Infrared (FTIR) spectra were collected using Thermo Scientific Nicolet FT-IR spectrometer with Diffuse Reflectance Infrared Transform Spectroscopy (DRIFTS) accessory.

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