Elisa coating solution
ELISA coating solution is a reagent used in enzyme-linked immunosorbent assay (ELISA) techniques. It is designed to coat the surface of ELISA plates, facilitating the immobilization of target antigens or antibodies for subsequent detection and quantification steps.
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4 protocols using elisa coating solution
Optimizing Anti-S Monoclonal Antibody for PEDV ELISA
Quantifying Osteocalcin and Alkaline Phosphatase
The cell lysates were diluted with PBS and 0.1 mL diluted cell lysate was added into appropriate wells. After the plates were incubated at 37°C for 2 h, the lysate was removed and washed four times with 200 μL PBS/ well. Then, 100 μL of the appropriate primary antibodies (1:3000) were added to each well and the plate was incubated at 37°C for 1 h. After washing four times with PBS, 100 μL HRP-linked secondary antibody was added to each well and the plate was incubated at 37°C for 30 min. After washing four times with PBS, 100 μL of TMB Substrate (SolarBio) was added to each well and the plate was incubated at 37°C for 10 min, and at 25°C for 30 min. Then 100 μL of ELISA STOP Solution (SolarBio) was added to each well and the plate was shaken gently for a few seconds. Finally, the absorbance at 450 nm was read using a BioRad microplate reader.
Antibody Response Analysis by ELISA
Quantifying Angiogenic Factors by ELISA
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