Log In Sign up
The largest database of trusted experimental protocols

Mplanfl n

Manufactured by Olympus
Visit Supplier
Sourced in Japan

The MPlanFL N is a metallurgical microscope objective lens designed for Olympus microscopes. It provides high-quality images for the observation and analysis of metal and other opaque specimens.

Automatically generated - may contain errors

Lab products found in correlation

Axio 10, by Zeiss (2 mentions) Bx53m, by Olympus (2 mentions) Ultrafluar, by Zeiss (1 mentions) Alpha 300, by WITec (1 mentions) Ihr550, by Horiba (1 mentions) Infinity 2, by OceanOptics (1 mentions) Qe65000, by Olympus (1 mentions) Pm100d, by Thorlabs (1 mentions) Polygon400, by Mightex (1 mentions) Labview software, by National Instruments (1 mentions) U mwib3, by Olympus (1 mentions) Xl30 sfeg, by Thermo Fisher Scientific (1 mentions) Helios nanolab 650, by Thermo Fisher Scientific (1 mentions) Dimension 3100, by Veeco (1 mentions)

13 protocols using mplanfl n

1

Optical Characterization of Reflected Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
Reflection color images of the sample were characterized using an objective lens (MPlanFL N, ×50×/0.8, Olympus Co.). A CCD camera (Olympus, BX53, Olympus Co) was used to acquire the images from the sample. The spectra were characterized with a home built confocal microscope coupled to a spectrometer (Andori500). The sample was illuminated using a halogen white light source using an objective lens (MPlanFL N, ×20×/0.45, Olympus Co.). The reflected light was collected through the same objective lens and recorded using a spectrometer. The reflected intensity was normalized by the spectrum of the lamp obtained by reflection measurements with a silver mirror.
Hu D., Li H., Zhu Y., Lei Y., Han J., Xian S., Zheng J., Guan B.O., Cao Y., Bi L, & Li X. (2021). Ultra-sensitive nanometric flat laser prints for binocular stereoscopic image. Nature Communications, 12, 1154.
+ Open protocol
+ Expand
2

Optical Characterization of Nanostructures

Check if the same lab product or an alternative is used in the 5 most similar protocols
An optical microscope (Carl-Zeiss Axio-10) equipped with ×5 (0.13 NA), ×10 (0.25 NA), ×20 (0.4 NA), ×50 (0.75 NA), and ×100 (0.85 NA) objective lens is used to obtain the reflection and transmission optical micrographs with different magnifications. The transmittance spectra under normal incidence were collected by an Olympus microscope (BX-51) with a spectrometer through a ×100 objective (MPlan-FLN, 0.9 NA). The extinction spectra were measured in transmission mode using a 2030 PV ultraviolet–visible–near-infrared range microspectrophotometer (CRAIC Technology Inc.) equipped with a xenon light source (80 W) and an optical objective (ZEISS Ultrafluar, ×10, 0.2 NA). Both the incident and collected light were normal to the quartz substrate, thus providing linearly polarized light excitation in plane with the surface of the nanostructures.
Zheng M., Chen Y., Liu Z., Liu Y., Wang Y., Liu P., Liu Q., Bi K., Shu Z., Zhang Y, & Duan H. (2019). Kirigami-inspired multiscale patterning of metallic structures via predefined nanotrench templates. Microsystems & Nanoengineering, 5, 54.
+ Open protocol
+ Expand
3

SERS Imaging of Plasmonic Nanostructures

Check if the same lab product or an alternative is used in the 5 most similar protocols
SERS imaging was performed with a confocal Raman microscope (WITec alpha300) equipped with an upright optical microscope. For excitation a 532 nm laser was used that was coupled into a single-mode optical fiber and focused through a 100× objective (Olympus MPlanFL N, NA = 0.9) to a diffraction-limited spot (1.3 μm2) on the Si sample. Due to the size of the laser spot only NP structures without any other potential SERS active NPs in the vicinity of 0.65 μm radius have been analyzed. The laser power and the integration time were set to 900–1000 μW and 2 s for dye-covered AuNPs (structures 1, 2) or to 400–500 μW and 10 s in the case of single-molecule measurements (structures 4a, 4b). The grating of the spectrograph was set to 600 g mm–1. For better visualization SERS spectra are vertically shifted in all diagrams presenting more than one spectrum.
Prinz J., Heck C., Ellerik L., Merk V, & Bald I. (2016). DNA origami based Au–Ag-core–shell nanoparticle dimers with single-molecule SERS sensitivity †Electronic supplementary information (ESI) available: Additional information about materials and methods, designs of DNA origami templates, height profiles, additional SERS spectra, assignment of DNA bands, SEM images, additional AFM images, FDTD simulations, additional reference spectra for Cy3 and detailed description of EF estimation, simulated absorption and scattering spectra. See DOI: 10.1039/c5nr08674d Click here for additional data file.. Nanoscale, 8(10), 5612-5620.
+ Open protocol
+ Expand
4

Dark-Field Scattering Mapping and Spectroscopy

Check if the same lab product or an alternative is used in the 5 most similar protocols
The dark-field scattering mapping and spectra were measured with a commercial hyperspectral imaging system (Cytoviva, HISV3). The white light was focused by a 100× objective with a high NA (Olympus, MPlanFLN, NA = 0.9). The mapping of the scattering signal was realized with a precise motorized translation stage, and spectral profiles of all pixels could be obtained. Scattering signals were recorded by a spectrometer (Horiba, iHR550) cooled to −60 °C. Scattering spectra of samples were corrected with the substrate using build-in software (Cytoviva, ENVI 4.8).
Qi P., Dai Y., Luo Y., Tao G., Zheng L., Liu D., Zhang T., Zhou J., Shen B., Lin F., Liu Z, & Fang Z. (2022). Giant excitonic upconverted emission from two-dimensional semiconductor in doubly resonant plasmonic nanocavity. Light, Science & Applications, 11, 176.
+ Open protocol
+ Expand
5

Confocal Raman Microscopy Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
For Raman measurements a drop of the corresponding was applied to a freshly cleaned glass slide. Raman measurements have been performed using a confocal Raman microscope (WITec 300α) equipped with an upright optical microscope. The excitation laser light at 532 nm was coupled into a single-mode optical fiber and focused through a 100x objective (Olympus MPlanFL N, NA = 0.9) to a diffraction-limited spot of about 1.3 μm2. The laser power was set to 13 mW and the integration time was 10 s for all measurements. Each spectrum has been obtained by an average of three accumulations. The spectra that cover a broad range of wavenumbers have been recorded using a grating with 600 g/mm whereas for the more detailed measurement a grating with 1800 g/mm has been used.
Oertel J., Keller A., Prinz J., Schreiber B., Hübner R., Kerbusch J., Bald I, & Fahmy K. (2016). Anisotropic metal growth on phospholipid nanodiscs via lipid bilayer expansion. Scientific Reports, 6, 26718.
+ Open protocol
+ Expand
6

SERS Spectra Acquisition Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
SERS spectra
have been recorded using a confocal Raman microscope (WITec 300α)
equipped with an upright optical microscope. For Raman excitation,
laser light at λ = 633 nm was used that was coupled into a single-mode
optical fiber and focused through a 50× objective (Olympus MPlanFL
N, NA = 0.75) to a spot size of about 1000 nm. The laser power was
varied (100, 200, 500, and 1000 μW) at the focal plane, and
the integration time was 1 s. The kinetics were followed between 5
to 15 min for each sample, i.e., each curve is based on 300–900
different spectra. Each sample was measured at least 3 times, and
the results shown here are the average of all the different Raman
measurements, i.e., all the spectra collected were averaged over time,
and the time traces are extracted directly from the average data.
Kogikoski S J.r., Dutta A, & Bald I. (2021). Spatial Separation of Plasmonic Hot-Electron Generation and a Hydrodehalogenation Reaction Center Using a DNA Wire. ACS Nano, 15(12), 20562-20573.
+ Open protocol
+ Expand
7

Optical Microscopy of GT Resonators

Check if the same lab product or an alternative is used in the 5 most similar protocols
The completed GT resonator with NPs was mounted onto the stage of optical microscopy system (BX53M, Olympus, Tokyo, Japan). Optical bright-field micrographs were captured using a 100× objectives lens (MPlanFLN, Olympus, Japan) and a computer-connected CMOS camera (STC-MCCM200U3V, Omron Sentech, Ebina, Japan) under a white LED lamp as a continuous light source.
Kang J., Yoo Y.J., Ko J.H., Mahmud A.A, & Song Y.M. (2023). Trilayered Gires–Tournois Resonator with Ultrasensitive Slow-Light Condition for Colorimetric Detection of Bioparticles. Nanomaterials, 13(2), 319.
+ Open protocol
+ Expand
8

Optical Imaging and Spectroscopy of Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
Optical BF/DF images and spectra of samples were obtained using a CCD camera (Infinity 2) and spectrometer (Ocean Optics QE65000) with 100 × objectives (Olympus MPlanFLN) in a customized microscope (Olympus BX51). A halogen lamp (Philip 7023) was used as the white light source. Reflection and transmission measurements were conducted in BF configuration, and scattering measurements were conducted in DF configuration.
Peng J., Jeong H., Smith M., Chikkaraddy R., Lin Q., Liang H., De Volder M.F., Vignolini S., Kar‐Narayan S, & Baumberg J.J. (2020). FullyPrinted Flexible Plasmonic Metafilms with Directional Color Dynamics. Advanced Science, 8(2), 2002419.
+ Open protocol
+ Expand
9

High-resolution Optogenetic Mapping of Neural Circuits

Check if the same lab product or an alternative is used in the 5 most similar protocols
Optical stimulation was implemented using a digital micromirror device (DMD) coupled to a 463 nm CW laser (Polygon 400, Mightex Systems). The stimulus consisted of a 1000 x 500 µm grid divided into 24 x 12 spots of light (41.7 µm x 41.7 µm square) delivered through a 5x/0.15 NA objective (Olympus MPlanFL N). The grid was centered on the soma and aligned to the pia orthogonal to the apical dendrite. The laser output associated with each spot was measured (PM100D and S121C, Thorlabs) and adjusted to obtain a measured power of approximately 300 µW (173 mW/mm2). Optical stimuli were delivered for 1 ms at 10 Hz in a pseudo-random sequence designed to maximize the distance between consecutive spots and the time between stimulation of neighboring spots. Each recording trial consisted of a single repetition of all 288 stimuli followed by a full-field stimulus, in which all stimulation spots were illuminated simultaneously for 1 ms. 5-20 trials were recorded, with 30s pauses between trials, making the interval between consecutive stimulation of the same spot 60s. An image of the recorded cell (filled with Alexa Fluor 488) relative to the stimulation grid was used during analysis to align the recorded sCRACM heatmap with the location of the pia or soma.
Galloni A.R., Ye Z, & Rancz E. (2022). Dendritic Domain-Specific Sampling of Long-Range Axons Shapes Feedforward and Feedback Connectivity of L5 Neurons. The Journal of Neuroscience, 42(16), 3394-3405.
+ Open protocol
+ Expand
10

Optical Characterization of Microscale Materials

Check if the same lab product or an alternative is used in the 5 most similar protocols
The transmittance spectra under normal incidence were acquired by Olympus microscope (BX-51) equipped with a spectrometer through a 100 × objective (MPlan-FLN, NA = 0.9). An optical microscope (Carl-Zeiss AXIO-10) with ×5 (0.13 NA), ×10 (0.25 NA), ×20 (0.4 NA), ×50 (0.75 NA), and ×100 (0.85 NA) objective lens is used to obtain the transmission optical micrographs with different magnifications.
Wang Y., Zheng M., Ruan Q., Zhou Y., Chen Y., Dai P., Yang Z., Lin Z., Long Y., Li Y., Liu N., Qiu C.W., Yang J.K, & Duan H. (2018). Stepwise-Nanocavity-Assisted Transmissive Color Filter Array Microprints. Research, 2018, 8109054.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!