Human mmp9 elisa kit

S100B, NSE, IL-6, IL-10, IL-18, and MMP-9 were measured according to the kit's instructions. The absorbance of each well was measured at 450 nm using a microplate reader. The human IL-18 ELISA kit (D7620; R&D Systems, Minneapolis, MN, USA), human IL-10 ELISA kit (D1000B; R&D Systems, Minneapolis, MN, USA), human MMP9 ELISA kit (D6000; R&D Systems, Minneapolis, MN, USA), human S100B ELISA kit (DY1820-05; R&D Systems, Minneapolis, MN, USA), and human NSE ELISA kit (DENL20; R&D Systems, Minneapolis, MN, USA) were used.

Serum levels of proBDNF, mature BDNF, and MMP-9 were measured using the human proBDNF Rapid ELISA Kit (Biosensis, Thebarton, SA, Australia), the human BDNF ELISA Kit (Aviscera Bioscience, Santa Clara, CA), and the human MMP-9 ELISA Kit (R&D Systems, Minneapolis, MN), respectively. To minimize assay variance, serum levels of proBDNF, mature BDNF, and MMP-9 were measured in each subject in a single day. All measurements were performed in duplicates and the protocols were performed according to the instructions provided by the manufacturers. The optical density of each well was measured using an automated microplate reader (Emax; Molecular Devices, Sunnyvale, CA).

Blood samples of humans or rats containing the anticoagulant EDTA were centrifuged at 3000 g for 10 min at room temperature, and then the upper two-thirds of the plasma were carefully removed for testing. The samples were centrifuged at 500 g for 5 min, and the upper two-thirds of the cell culture supernatant were carefully aspirated for testing. The experimental steps were performed according to the instructions of the corresponding ELISA kit. The following kits were used: human HBP ELISA kit (Thermo Fisher, USA), human MPO ELISA Kit (R&D Systems, USA), human elastase ELISA kit (R&D Systems, USA), human MMP9 ELISA kit (R&D Systems, USA), human hyaluronic acid ELISA kit (Biorbyt, UK), human heparan sulfate ELISA kit (Biorbyt, UK), human SDC-1 ELISA kit (Biorbyt, UK), rat HA ELISA kit (Cusabio, China), rat HS ELISA kit (Cusabio, China), and rat SDC-1 ELISA kit (Cusabio, China).