Silibinin, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), N-acetylcysteine (NAC), ascorbic acid (AA), 3-methyladenine (3-MA), bafilomycin-A1 (Baf-A1), mouse anti-human BNIP3 and rabbit anti-human light chain 3 (LC3) were all obtained from Sigma-Aldrich. Antibodies for rabbit anti-human Bcl-2, β-actin, rabbit anti-human Atg12 and rabbit anti-human Beclin-1 were all purchased from Cell Signaling Technology (Beverly, MA, USA). 5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethylbenzimidazol ylcarbocyanine iodide (JC-1) and dihydroethidium (DHE) were obtained from Molecular Probes. BNIP3 siRNA was purchased from RiboBio Co. (guangdong, China).
5 5 6 6 tetrachloro 1 1 3 3 tetraethylbenzimidazolylcarbocyanine iodide jc 1
Manufactured by Thermo Fisher Scientific
Sourced in United States
5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine iodide (JC-1) is a fluorescent dye used in cell biology research. It is a lipophilic cation that can selectively enter mitochondria and exhibits potential-dependent accumulation, indicated by a fluorescence emission shift from green to red. This property allows JC-1 to be used as an indicator of mitochondrial membrane potential.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (8 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (4 mentions)
β actin, by Cell Signaling Technology (3 mentions)
Antimycin a, by Merck Group (3 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (3 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (3 mentions)
N acetylcysteine, by Merck Group (3 mentions)
Hoechst 33342, by Merck Group (3 mentions)
Bovine serum albumin (bsa), by Merck Group (3 mentions)
Mitosox, by Thermo Fisher Scientific (2 mentions)
2 7 dichlorofluorescin diacetate dcfh da, by Thermo Fisher Scientific (2 mentions)
Real time pcr reagents, by Thermo Fisher Scientific (2 mentions)
Cytochrome c immunoassay kit, by R&D Systems (2 mentions)
Cellrox deep red reagent, by Thermo Fisher Scientific (2 mentions)
Sucrose, by Merck Group (2 mentions)
Valinomycin, by Merck Group (2 mentions)
Hrp conjugated goat anti rabbit igg, by Santa Cruz Biotechnology (2 mentions)
Rotenone, by Merck Group (2 mentions)
Cytochrome c, by Cell Signaling Technology (2 mentions)
Cleaved parp, by Cell Signaling Technology (2 mentions)
30 protocols using 5 5 6 6 tetrachloro 1 1 3 3 tetraethylbenzimidazolylcarbocyanine iodide jc 1
1
Silibinin-Induced Autophagy Assessment
2
Artemisinin Cytotoxicity and Oxidative Stress in Alzheimer's
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Analytical grade artemisinin was purchased from Meilunbio (Dalian China). Dimethyl sulfoxide (DMSO) and sucrose were procured from Sigma-Aldrich (St. Louis, MO, USA). Hoechst 33258, 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl-benzimidazolyl-carbocyanineiodide (JC-1), and Poly-D-lysine, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), were obtained from Molecular Probes (Eugene, OR, USA). CellROX Deep Red Reagent was ordered from Thermo Fisher Scientific (Rockford, IL, USA). DCFH-DA reagent was ordered from Beyotime Institute of Biotechnology (Shanghai, China). Annexin V-FITC/PI Apoptosis Detection Kit was obtained from BD Biosciences (San Diego, CA, USA). Trypsin (0.25%) and fetal bovine serum (FBS) and 0.25% were procured from Life Technologies (Grand Island, NY, USA). MEK/ERK inhibitor PD98059 was ordered from Calbiochem (San Diego, CA, USA). The information of antibodies was shown in Table 1 . The detail information of β-Amyloid (1-42) was provided in Table 2
3
Mitochondrial Function Assays
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All reagents, unless otherwise stated, are from Merck (Darmstadt, Germany). ATP Bioluminescent Assay Kit, 7′-dichlorodihydrofluorescein diacetate (H2DCFDA), antimycin A, valinomycin, protease inhibitor cocktail, and phosphatase inhibitor cocktail were from Sigma Aldrich (St. Louis, MO, USA). 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine iodide (JC-1) and MitoSOX™ were from Molecular Probes (Invitrogen, Carlsbad, CA, USA).
4
Comprehensive Molecular Profiling of Cell Death
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3-Bromopyruvate (3BP), ATP Bioluminescent Assay Kit, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA), MitoTEMPO, antimycin A, menadione, perifosine, digitonin, staurosporine, erythrosin B, LC3 rabbit polyclonal antibody, and actin mouse monoclonal antibody were from Sigma Aldrich. Cyt c mouse monoclonal, COX-IV mouse monoclonal, p53 mouse monoclonal, goat anti-mouse HRP-conjugated IgG, goat anti-rabbit HRP-conjugated IgG, and rabbit anti-goat HRP-conjugated IgG antibodies were from Santa Cruz Biotechnology. Akt rabbit polyclonal, p-Akt (Ser-473) rabbit monoclonal, JNK rabbit monoclonal, p-JNK (Thr183/Tyr185) rabbit monoclonal, p44/42 MAPK (ERK1/2) rabbit monoclonal, p-ERK1/2 (Thr202/Tyr204) rabbit monoclonal, p38 MAPK rabbit polyclonal, p-p38 (Thr180/Tyr182) rabbit monoclonal, p-p53 (Ser-15) rabbit polyclonal, caspase-3 rabbit polyclonal, and caspase-9 rabbit polyclonal antibodies were from Cell Signaling Technology. Beta tubulin mouse monoclonal antibody was from Proteintech. 5,5′,6,6′-tetrachloro-1,1′,3,3′tetraethylbenzimidazolylcarbocyanine iodide (JC-1) and MitoSOX™ were from Molecular Probes.
5
Cardioprotective Effects of DHAG on H9c2 Cells
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The cardiomyoblast cell line H9c2 was purchased from Beijing Cell bank (Beijing, China). Fetal bovine serum and RPMI-1640 medium were from Gibco BRL (Gaithersburg, MD, USA). LTA and 3-(4,5-dimethylthiazol)-2,5-diphenyltetrazolium-bromide (MTT) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Caspase-3/9 activity assay kits were from Promega (Madison, WI, USA). Glutathione (GSH), superoxide dismutase (SOD), malondialdehyde (MDA), IL-1β, and TNF-α assay kits were purchased from Jiancheng Biological Engineering (Nanjing, China). The cytochrome c assay kit was from R&D systems (Minneapolis, MN, USA). 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine iodide (JC-1) and 2′,7′-dichlorofluorescin diacetate (DCFH-DA) were purchased from Molecular Probes (Eugene, OR, USA). Real-time PCR reagents were purchased from Thermo Fisher (Waltham, MA, USA). DHAG was provided by Prof. Teng from Zhengzhou University.15 All solvents and chemicals were analytical grade and were purchased from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). LTA and DHAG were dissolved in dimethyl sulfoxide (DMSO).
6
Cytotoxic Effects of DOX and PD
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DOX and dimethyl sulfoxide (DMSO) were obtained from Sigma (St. Louis, MO, USA). DOX was dissolved in DMSO at a concentration of 40 mM, and stored at −20°C. PD was purchased from Chengdu Best Reagent Co., LTD (Chengdu, Sichuan, China), and dissolved in DMSO at a concentration of 40 mM and stored at −20°C. 3-[4,5-Dimethyl-2-thiazolyl]-2,5-diphenyltetrazolium bromide (MTT) and 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimi-dazolylcarbocyanine iodide (JC-1) were purchased from Molecular Probes (Eugene, OR, USA). RPMI 1640 medium, fetal bovine serum (FBS) and antibiotics were purchased from Gibco (Carlsbad, CA, USA). Primary antibodies [i.e., poly (ADP-ribose) polymerase (PARP) and β-actin], and secondary antibodies were obtained from Cell Signaling Technology (Beverly, MA, USA).
7
Cardiomyoblast Stress Response Protocol
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H9c2 cardiomyoblast cell line was purchased from Shanghai Cell Bank (Shanghai, China). LTA was purchased from National Institutes for Food and Drug Control (Beijing, China). Fetal bovine serum (FBS) and Dulbecco’s Modified Eagle’s medium (DMEM) were purchased from Gibco BRL (Gaithersburg, USA). Caspase-3/9 activity assay kits and 3-(4,5-dimethylthiazol)-2,5-diphenyltetrazolium-bromid (MTT) were purchased from Sigma-Aldrich (St. Louis, USA). MDA, GSH, SOD, IL-1β, IL-12, and TNFα assay kits were purchased from Jiancheng Biological Engineering (Nanjing, China). Cytochrome-c immunoassay kit was purchased from R&D systems (Minneapolis, USA). 5,5’,6,6’-tetrachloro-1,1’,3,3’-tetraethylbenzimidazolylcarbocyanine iodide (JC-1) and 2’,7’-dichlorofluorescin diacetate (DCFH-DA) were purchased from Molecular Probes (CA, USA). Gamma H2AX (γH2AX) antibody was purchased from BioLegend (San Diego, USA). Real-time PCR reagents were purchased from Thermo Fisher (Waltham, MA, USA). TIM was isolated and identified by Prof. Lin from Shantou University Medical College (Shantou, China) (14 ). All solvents and chemicals used in this study were of analytical grade and purchased from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). LTA and TIM were dissolved in DMSO in the in vitro experiments.
8
Mitochondrial Function Profiling Protocol
9
Colorectal Cancer Cell Culture Protocol
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SW480, SW620, and LS411N colorectal-cancer cells were obtained from American Type Culture Collection (Manassas, VA, USA) and cultured in Dulbecco’s modified eagle medium (DMEM; Gibco; Carlsbad, CA, USA) containing 10% v/v fetal bovine serum (FBS) and 1% penicillin-streptomycin (Sigma; St. Louis, MO, USA) at 37°C in a 5% CO2 humidified incubator. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was obtained from Sigma. An Annexin V-fluorescein isothiocyanate/propidium iodide (Annexin V-FITC/PI) kit was purchased from BD Pharmingen (Franklin Lakes, NJ, USA). 5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine iodide (JC-1) was obtained from Molecular Probes, Inc. (Eugene, OR, USA). Transwell plates were obtained from Corning Inc. (Corning, NY, USA). Caspase-3, PARP (9542S), Bcl-2 (2870S), STAT3 (9139S), p-STAT3 (9145S), Bim (2819S), Bak (6947S), MCL-1 (4572S), E-cadherin (3195S), Vimentin (3932S), and β-actin were purchased from Cell Signaling Technology (Boston, MA, USA). Stattic, the STAT3 inhibitor, was obtained from Selleck Chemicals (Houston, TX, USA).
10
Mitochondrial Function and Oxidative Stress Assays
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