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Glyceraldehyde 3 phosphate dehydrogenase gapdh antibody 6c5

Manufactured by Santa Cruz Biotechnology
Sourced in United States

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody (6C5) is a primary antibody that recognizes the GAPDH protein. GAPDH is an enzyme involved in the glycolytic pathway and is commonly used as a loading control or reference protein in various biochemical and cell biology experiments.

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2 protocols using glyceraldehyde 3 phosphate dehydrogenase gapdh antibody 6c5

1

Analyzing Cellular Responses with Rhodamine 123 and L-NAME

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Rhodamine 123, l‐NAME, and sodium dodecyl sulfate (SDS) were purchased from Wako (Tokyo, Japan). ISOGEN was purchased from Nippongene (Tokyo, Japan). DMEM, RIPA buffer, protease inhibitor cocktail, and β‐Actin antibody were purchased from Sigma (MO, USA). Fetal bovine serum (FBS) and house serum (HS) were purchased from Gibco (NY, USA). MTT or 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide and l‐NAME were purchased from Dojindo (Kumamoto, Japan). l‐citrulline was supplied by Kyowa Hakko Bio Co., Ltd., Tokyo, Japan) while PGC‐1α (3G6) antibody was purchased from Cell Signaling Technology (Hertfordshire, UK). Glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) antibody (6C5) was purchased from Santa Cruz Biotechnology (CA, USA).
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2

Molecular Mechanisms of Adipogenesis

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Cy3G was purchased from Tokiwa Phytochemical Co. Ltd. (Chiba, Japan). ISOGEN was purchased from Nippongene (Tokyo, Japan). Rhodamine 123 and sodium dodecyl sulphate (SDS) were purchased from Wako (Tokyo, Japan). DMEM, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), Triton X-100, RIPA buffer, protease inhibitor cocktail, β-Actin antibody, L-lactic acid, 3-isobutyl-1-methylxanthine (IBMX) and Ro 20-1724 were purchased from Sigma (MO, USA). Fetal bovine serum, house serum, Hanks’ balanced salt solution (HBSS) and DMEM (no glucose) were purchased from Gibco (NY, USA). MTT was purchased from Dojindo (Kumamoto, Japan). STO-609 was purchased from Focus Biomolecules (PA, USA). PGC-1α (3G6), AMPKα and phospho-AMPKα (Thr172) antibodies were purchased from Cell Signaling Technology (Hertfordshire, UK). Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody (6C5) was purchased from Santa Cruz Biotechnology (CA, USA).
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