Soybean lecithin s100

Soybean lecithin (S100) was provided by Lipoid GmbH (Ludwigshafen, Germany). Folic acid, oleic acid, cholesterol, chitosan (molecular weight ~110,000–150,000), N-hydroxysuccinimide (NHS), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), and cobalt chloride were purchased from Sigma-Aldrich (St Louis, MO, USA). HIF-1α siRNA (human), control HIF-1α siRNA (fluorescein isothiocyanate [FITC] conjugate)-A, anti-HIF-1α antibody, goat anti-rabbit IgG-HRP, and GAPDH antibody were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Deionized water was prepared by a water purifying system (Woter, Chengdu, People’s Republic of China). All other chemicals were of analytical grade and used as received. The protocol of cell experiments was reviewed and approved by the Medical Ethics Committee of Shanghai Dermatology Hospital.

Docetaxel was purchased from ScinoPharm Taiwan (Tainan, Taiwan). Soybean lecithin (S 100) was provided by Lipoid (Ludwigshafen, Germany). 1.2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DSPE-PEG2000) and 1.2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DSPE-PEG5000) were purchased from NOF (Tokyo, Japan). Kolliphor^® TPGS was obtained from BASF (Florham Park, NJ, USA). 3.30-Dioctadecyloxacarbocyanine perchlorate (DIO) was obtained from Sigma-Aldrich (St. Louis, MO, USA). Tynen^® (docetaxel), a generic product, was provided by TYY Pharmaceutical (Taipei, Taiwan). Fetal bovine serum, non-essential amino acids and sodium pyruvate were obtained from Thermo Fisher Scientific (Waltham, MA, USA). Dulbecco’s Modified Eagle Medium (DMEM), Minimum Essential Medium (MEM) and penicillin/streptomycin/amphotericin B solution were purchased from Corning Incorporated (Corning, NY, USA). Other reagents and solvents were of analytical grade. WS1, MIA PaCa-2 and HT-29 were supplied by American Type Culture Collection (Manassas, VA, USA). WS1/FAP cells were human WS1 fibroblasts engineered to overexpress human FAP by lentiviral infection.

Doxorubicin hydrochloride
(DOX) was obtained from Huafeng United Technology (Beijing, China,
purity ≥95%). Docosahexaenoic acid (DHA, C22:6n-3) was purchased
from Nu-Chek (Eslyan, Minnesota, purity ≥95%). Soybean lecithin
(S100) was obtained from Lipoid (Ludwigshafen, Germany). Soybean oil
was provided by Beiya Medical Oil Co., Ltd. (Tieling, China). Pluronic
F68 was kindly offered by BASF (Ludwigshafen, Germany). 3-(4,5-Dimethylthiazol-2-yl)-2,
5-diphenyltetrazolium bromide (MTT), lipopolysaccharides (LPSs), and
4,6-diamidino-2-phenylindole (DAPI) were purchased from Sigma-Aldrich
(St. Louis, Missouri). Other chemicals and reagents were of analytical
grade or above.