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Soybean lecithin s100

Manufactured by Lipoid
Sourced in Germany

Soybean lecithin (S100) is a naturally-derived emulsifier and surfactant. It is extracted from soybeans and is composed of a mixture of phospholipids, including phosphatidylcholine, phosphatidylethanolamine, and phosphatidylinositol. Soybean lecithin (S100) is commonly used in various industrial and pharmaceutical applications to improve the stability and dispersion of formulations.

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5 protocols using soybean lecithin s100

1

Soybean Lecithin-based Nanoparticles for HIF-1α Silencing

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Soybean lecithin (S100) was provided by Lipoid GmbH (Ludwigshafen, Germany). Folic acid, oleic acid, cholesterol, chitosan (molecular weight ~110,000–150,000), N-hydroxysuccinimide (NHS), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), and cobalt chloride were purchased from Sigma-Aldrich (St Louis, MO, USA). HIF-1α siRNA (human), control HIF-1α siRNA (fluorescein isothiocyanate [FITC] conjugate)-A, anti-HIF-1α antibody, goat anti-rabbit IgG-HRP, and GAPDH antibody were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Deionized water was prepared by a water purifying system (Woter, Chengdu, People’s Republic of China). All other chemicals were of analytical grade and used as received. The protocol of cell experiments was reviewed and approved by the Medical Ethics Committee of Shanghai Dermatology Hospital.
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2

Docetaxel Nanoparticle Formulation and Cell Lines

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Docetaxel was purchased from ScinoPharm Taiwan (Tainan, Taiwan). Soybean lecithin (S 100) was provided by Lipoid (Ludwigshafen, Germany). 1.2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DSPE-PEG2000) and 1.2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DSPE-PEG5000) were purchased from NOF (Tokyo, Japan). Kolliphor® TPGS was obtained from BASF (Florham Park, NJ, USA). 3.30-Dioctadecyloxacarbocyanine perchlorate (DIO) was obtained from Sigma-Aldrich (St. Louis, MO, USA). Tynen® (docetaxel), a generic product, was provided by TYY Pharmaceutical (Taipei, Taiwan). Fetal bovine serum, non-essential amino acids and sodium pyruvate were obtained from Thermo Fisher Scientific (Waltham, MA, USA). Dulbecco’s Modified Eagle Medium (DMEM), Minimum Essential Medium (MEM) and penicillin/streptomycin/amphotericin B solution were purchased from Corning Incorporated (Corning, NY, USA). Other reagents and solvents were of analytical grade. WS1, MIA PaCa-2 and HT-29 were supplied by American Type Culture Collection (Manassas, VA, USA). WS1/FAP cells were human WS1 fibroblasts engineered to overexpress human FAP by lentiviral infection.
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3

Doxorubicin-Docosahexaenoic Acid Nanoformulation

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Doxorubicin hydrochloride
(DOX) was obtained from Huafeng United Technology (Beijing, China,
purity ≥95%). Docosahexaenoic acid (DHA, C22:6n-3) was purchased
from Nu-Chek (Eslyan, Minnesota, purity ≥95%). Soybean lecithin
(S100) was obtained from Lipoid (Ludwigshafen, Germany). Soybean oil
was provided by Beiya Medical Oil Co., Ltd. (Tieling, China). Pluronic
F68 was kindly offered by BASF (Ludwigshafen, Germany). 3-(4,5-Dimethylthiazol-2-yl)-2,
5-diphenyltetrazolium bromide (MTT), lipopolysaccharides (LPSs), and
4,6-diamidino-2-phenylindole (DAPI) were purchased from Sigma-Aldrich
(St. Louis, Missouri). Other chemicals and reagents were of analytical
grade or above.
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4

Formulation and Characterization of DTX Nanoparticles

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DTX was supplied by Qilu Pharmaceutical (Jinan, China). DSPE-PEG2K was purchased from NOF (Tokyo, Japan). DSPE-PEG5K was obtained from Avanti Polar Lipids. (Alabaster, AL). 3,3′-Dioctadecyloxacarbocyanine perchlorate (DIO), nystatin, and sucrose were obtained from Sigma-Aldrich (St. Louis, MO). 1,1’-Dioctadecyltetramethyl indotricarbocyanine iodide) (DIR) was purchased from Perkin-Elmer (Waltham, MA). Soybean lecithin (Lipoid S-100) was obtained from Lipoid GmbH (Ludwigshafen, Germany). Chlorpromazine and cytochalasin D were supplied by Cayman Chemicals (Ann Arbor, MI). Dynasore was obtained from MedChem Express (Monmouth Junction, NJ). LysoTracker Red DND-99 and Hoechst3342 were obtained from Thermo Fisher Scientific (Waltham, MA). The anti-PEG backbone mAb AGP4 was provided by Dr. Steve R. Roffler (Academia Sinica, Taipei, Taiwan). Tynen® (solvent-based DTX) is a generic product of DTX manufactured by TYY Pharmaceutical (lot no: STW1407, Taipei, Taiwan). All reagents were of analytical grade and solvents used in the high-performance liquid chromatography (HPLC) or ultra-performance liquid chromatography (UPLC)/mass spectrometry (MS/MS) analysis was of HPLC or MS grade.
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5

Silymarin and Irinotecan Formulation Study

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Silymarin (80%) was purchased from Sanjaing (Jiaxing, China). Irinotecan hydrochloride and SN-38 were purchased from Scino Pharm (Tainan, Taiwan). SN38G was purchased from Cayman Chemical (Ann Arbor, MI, USA). Rapamycin was purchased from Chunghwa Chemical Synthesis and Biotech (New Taipei City, Taiwan). Capryol-90 was purchased from Gattefosse (Lyon, France). Tween 80 and camptothecin were purchased from Merck KGaA (Darmstadt, Germany). Cremophor EL was procured from Wei Ming Pharmaceutical (Taipei, Taiwan). Ascomycin was purchased from MedChemExpress (South Brunswick, NJ, USA). Soybean lecithin (Lipoid S-100) was purchased from Lipoid GmbH (Ludwigshafen, Germany). Dulbecco’s modified Eagle’s medium, fetal bovine serum, and horse serum were purchased from Corning (New York, NY, USA). Reagents used for high-performance liquid chromatography (HPLC) or ultra-performance liquid chromatography with tandem mass spectrometry (UPLC/MS/MS) were of HPLC or MS grade, and other reagents were of analytical grade.
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