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Il 10 opteia elisa kit

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The IL-10 OptEIA ELISA kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) used for the measurement of interleukin-10 (IL-10) in biological samples. It is designed to detect and quantify IL-10 levels in the sample.

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BD OptEIA (350 citations) IL-10 (156 citations) OptEIA kit (130 citations) BD OptEIA ELISA kits (114 citations) ELISAs (6 citations)

4 protocols using il 10 opteia elisa kit

1

Quantifying IL-10 Secretion by Breg Cells

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Purified CD1dhighCD5+ Breg cells (1×105 cells/ml) were cultured in 1 ml of complete medium with or without LPI for 5 hrs. IL-10 concentration in culture supernatant was then quantified using the IL-10 OptEIA ELISA kit (BD Pharmingen, San Diego, CA, USA) following the manufacturer’s protocol. All assays were conducted in triplicate.
Yokoyama T., Yoshizaki A., Simon K.L., Kirby M.R., Anderson S.M, & Candotti F. (2015). Age-Dependent Defects of Regulatory B Cells in Wiskott-Aldrich Syndrome Gene Knockout Mice. PLoS ONE, 10(10), e0139729.
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2

Cytokine Profiling of Murine and Human CLL

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For cytokine analysis, normal B-1, murine CLL and human CLL cells were cultured in triplicate (2×106 cells/mL) for 24 hours in 96-well plate with various stimulants or treatments. Cells were removed by centrifugation and supernatants were assayed immediately or stored at −80°C. Plasma from mice was obtained by centrifuging blood collected in EDTA tubes at the time of euthanization by cardiac puncture and the samples were stored at −80°C. IL-10 levels in supernatants or plasma were quantified using IL-10 OptEIA ELISA kit (BD#555252). IFN-γ levels were measured using IFN-γ OptEIA ELISA kit (BD#555138). Human plasma or secreted IL-10 levels were quantified using IL-10 ELISA MAX set (BioLegend #430601). Limit of IL-10 detection is 8 pg/ml.
Alhakeem S.S., McKenna M.K., Oben K.Z., Noothi S.K., Rivas J.R., Hildebrandt G.C., Fleischman R.A., Rangnekar V.M., Muthusamy N, & Bondada S. (2018). Chronic lymphocytic leukemia derived interleukin-10 suppresses anti-tumor immunity. Journal of immunology (Baltimore, Md. : 1950), 200(12), 4180-4189.
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3

Stimulating B Cells with LPS

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Isolated splenic CD19+ B cells (3 × 106 cells/well) and Peritoneal cavity fluid (PeC)-derived CD19+ B cells (1 × 106 cells/well) were stimulated with LPS (0, 0.1, 1, and 10 μg/ml) for 24 or 48 h, the level of IL-10 by using a mouse BD OptEIA IL-10 ELISA kit according to the manufacturer’s instructions (BD Biosciences, San Jose, CA).
Lee M.B., Lee J.H., Hong S.H., You J.S., Nam S.T., Kim H.W., Park Y.H., Lee D., Min K.Y., Park Y.M., Kim Y.M., Kim H.S, & Choi W.S. (2017). JQ1, a BET inhibitor, controls TLR4-induced IL-10 production in regulatory B cells by BRD4-NF-κB axis. BMB Reports, 50(12), 640-646.
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4

Quantifying IL-10 Levels via ELISA

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IL-10 levels were quantified harnessing a BD OptEIA IL-10 ELISA Kit (BD Biosciences, USA) according to the manufacturer’s instructions.
Draxler D.F., Hanafi G., Zahra S., McCutcheon F., Ho H., Keragala C.B., Liu Z., Daly D., Painter T., Wallace S., Plebanski M., Myles P.S, & Medcalf R.L. (2022). Tranexamic acid alters the immunophenotype of phagocytes after lower limb surgery. Thrombosis Journal, 20, 17.
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