Pro prism 8

Manufactured by GraphPad

Sourced in United States

GraphPad Prism 8.0 is a software application for data analysis, curve fitting, and scientific graphing. It provides a user-friendly interface for data management, statistical analysis, and visualization of scientific data.

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Lab products found in correlation

Cell counting kit 8 cck 8, by Beyotime (1 mentions) Cck 8, by GraphPad (1 mentions)

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Prism 8 (45576 citations) GraphPad Prism Pro (14 citations)

12 protocols using pro prism 8

Statistical Analysis of Experimental Data

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Statistical analysis was performed using GraphPad Pro Prism 8.0 software (GraphPad Software, Inc.). All experiments were performed in triplicate and data are presented as the mean ± SEM. Unpaired Student's t-test was used to compare differences between two groups, while one-way ANOVA followed by Tukey's post hoc test were used to compare differences between multiple groups. P<0.05 was considered to indicate a statistically significant difference.

Feng D., Guo L., Liu J., Song Y., Ma X., Hu H., Liu J, & Hao E. (2021). DDX3X deficiency alleviates LPS-induced H9c2 cardiomyocytes pyroptosis by suppressing activation of NLRP3 inflammasome. Experimental and Therapeutic Medicine, 22(6), 1389.

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Statistical Analysis of Experimental Data

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Statistical analysis was conducted using GraphPad Pro Prism 8.0 software (GraphPad Software, Inc.). Statistical significance was evaluated by Unpaired Student's test for two groups and one‐way analysis of variance (ANOVA) for multiple groups. Every experiment was repeated three times. The data are presented as the mean ± SEM. Statistical significance was regarded as p < 0.05.

Feng D., Li J., Guo L., Liu J., Wang S., Ma X., Song Y., Liu J, & Hao E. (2022). DDX3X alleviates doxorubicin‐induced cardiotoxicity by regulating Wnt/β‐catenin signaling pathway in an in vitro model. Journal of Biochemical and Molecular Toxicology, 36(8), e23077.

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In Vitro and In Vivo Efficacy Assessment

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All in vitro data represented at least three independent experiments and in vivo data represented at least six independent experiments. All experimental data were expressed as mean ± SEM (standard error of mean). Statistical analyses were performed using GraphPad Pro Prism 8.0 (GraphPad, San Diego, CA). Unpaired two‐tailed Student t‐test or one‐way analysis of variance (ANOVA) followed by Bonferroni's multiple comparisons test was employed to analyze the differences between sets of data. p‐Value < 0.05 was considered significant.

Luo W., Lin K., Hua J., Han J., Zhang Q., Chen L., Khan Z.A., Wu G., Wang Y, & Liang G. (2022). Schisandrin B Attenuates Diabetic Cardiomyopathy by Targeting MyD88 and Inhibiting MyD88‐Dependent Inflammation. Advanced Science, 9(31), 2202590.

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Cytotoxicity and Colony Formation Assay

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Cell counting kit-8 (CCK8) (Beyotime Biotechnology, Shanghai, China) was used for cytotoxicity analysis. Cells (5 × 10³/well) were plated in 96-well plates and stimulated with drugs at different concentration gradients for 24 h. CCK-8 solution was added and incubated in an incubator for 3 h. The absorbance value was measured at 450 nm, and Cancer Chemopreventative Properties (IC50) as calculated via GraphPad Pro Prism8.0 (GraphPad, San Diego, CA, USA). As for colony formation assay, cells (1 × 10³/well) were plated in 12-well plates and cultured for 24 h. Stimulation with different drug concentrations was added and cultured for 7 days. Cloned cell colonies were fixed, stained, and imaged.

Luo L, & Xu G. (2022). Fascaplysin Induces Apoptosis and Ferroptosis, and Enhances Anti-PD-1 Immunotherapy in Non-Small Cell Lung Cancer (NSCLC) by Promoting PD-L1 Expression. International Journal of Molecular Sciences, 23(22), 13774.

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Comparative Statistical Analysis Protocol

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All results are reported in the
form of means ± SD in this study. Comparison between 2 groups
was confirmed by the unpaired 2-tailed t test. Pearson
correlation was computed to evaluate the associations between two
continuous variables. The statistical analysis was applied in GraphPad
Pro Prism 8.0 (GraphPad, USA). A statistically significant result
was obtained at P value < 0.05.

Lin K., Shi D., Wang A., Ge J., Cheng D, & Yan Y. (2024). Noninvasive Monitoring of Early Cardiac Fibrosis in Diabetic Mice by [68Ga]Ga-DOTA-FAPI-04 PET/CT Imaging. ACS Omega, 9(15), 17195-17203.

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Statistical Analysis of in vitro/vivo Data

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All in vitro or in vivo results were expressed in the form of means ± standard error of the mean (SEM) in this study. To identify group differences, unpaired student's t-test was applied (2 group studies). For >2 groups, we used one way-ANOVA with multiple comparisons (Bonferroni's correction). P < 0.05 was used as the criterion for determining statistical significance. All analyses were executed with GraphPad Pro Prism 8.0 (GraphPad, San Diego, CA).

Fang Z., Wu G., Sheng J., Ye B., Huang Z., Xu J., Zhang J., Han J., Han B, & Xu J. (2023). Gasdermin D affects aortic vascular smooth muscle cell pyroptosis and Ang II-induced vascular remodeling. Heliyon, 9(6), e16619.

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In Vitro Experimentation Protocols

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All in vitro experiments were performed at least triplicate. Data are expressed as mean ± SEM. All statistical analyses were performed using GraphPad Pro Prism 8.0 (GraphPad, San Diego, CA). Student’s t-test and two-way ANOVA were employed to analyze the statistical significance between two or more sets of data, respectively. P value <.05 was considered statistically significant.

Chen M., Qian C., Jin B., Hu C., Zhang L., Wang M., Zhou B., Zuo W., Huang L, & Wang Y. (2023). Curcumin analog WZ26 induces ROS and cell death via inhibition of STAT3 in cholangiocarcinoma. Cancer Biology & Therapy, 24(1), 2162807.

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Statistical Analysis of Experimental Data

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All data are expressed as means ± SD. Statistical analyses were performed using GraphPad Pro Prism 8.0 (GraphPad, San Diego, CA). The Student t test or 1-way analysis of variance followed by the multiple comparisons test with Bonferroni correction was used to analyze the differences between sets of data. A P value less than .05 was considered significant.

Liu X., Luo W., Chen J., Hu C., Mutsinze R.N., Wang X., Zhang Y., Huang L., Zuo W., Liang G, & Wang Y. (2022). USP25 Deficiency Exacerbates Acute Pancreatitis via Up-Regulating TBK1–NF-κB Signaling in Macrophages. Cellular and Molecular Gastroenterology and Hepatology, 14(5), 1103-1122.

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Comprehensive Statistical Analysis Protocol

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All data are expressed as the means ± SEM. Statistical analyses were performed using GraphPad Pro Prism 8.0 (GraphPad, San Diego, CA). Student's t test or one-way analysis of variance followed by the multiple comparisons test with Bonferroni correction were used to analyze the differences between sets of data. We used one-way analysis of variance followed by multiple comparisons test with Bonferroni correction when comparing data from more than 2 groups and Student's t test when comparing data from 2 groups. A P value <0.05 was considered significant.

Xu J., Zhang B., Chu Z., Jiang F, & Han J. (2021). Wogonin Alleviates Cisplatin-induced Cardiotoxicity in Mice Via Inhibiting Gasdermin D-mediated Pyroptosis. Journal of Cardiovascular Pharmacology, 78(4), 597-603.

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Detailed Experimental Protocols for Scientific Research

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All data represented at least three independent experiments. All experimental data were expressed as mean ± standard deviation (SD). Unpaired two‐tailed Student t‐test or one‐way analysis of variance (ANOVA) followed by Bonferroni's multiple comparisons test was employed to analyze the differences between sets of data. p‐Value < 0.05 was considered significant. (*p < 0.05, **p < 0.01, ***p < 0.001, ^****p < 0.0001, ns: no significant). Some results were normalized to the control to avoid sources of variation. Statistical analyses were performed using GraphPad Pro Prism 8.0 (GraphPad, San Diegao, CA).

Fan C., Zhang Z., Lai Z., Yang Y., Li J., Liu L., Chen S., Hu X., Zhao H, & Cui S. (2023). Chemical Evolution and Biological Evaluation of Natural Products for Efficient Therapy of Acute Lung Injury. Advanced Science, 11(7), 2305432.

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