Prism vs 7

Statistical analysis was performed with GraphPad Prism vs7. Values are shown as mean of all replicates ± standard error of the mean (S.E.M) in which the number of independent experiments was equal or more than three. Values were analyzed by ANOVA with Dunnett’s post-test or by students’ t-test. A p value less than 0.05 was considered statistically significant. Where the number of independent experiments was less than three, values shown are mean ± range.
To study the effect of drug combination on cells toxicity or viability, excess over bliss (EOB) analysis was performed to determine the drug combination effects at each combination dose according to Liu et al. [29 (link)]. An EOB score > 1 is considered synergism, a score of >0 but <1 is considered independent/additive while a score ˂ 0 is considered antagonism.

GraphPad Prism vs.7 (San Diego, CA, USA) was used for statistical analyses. All data sets were analyzed for significant outliers using the ROUT method. Statistics is provided for both presence and absence of the significant outliers. D’Agostino & Pearson omnibus normality test was used to test data sets for Gaussian distribution before conducting comparisons of groups. Mouse groups from Aβ-measurements (histological and biochemical) and the Mesoscale cytokine ELISA assay were compared using Two-way ANOVA and Fisher’s LSD test for planned comparisons accompanied with correction for multiple comparisons for each experiment: The significance level was calculated by dividing the chosen alpha level (0.05) with the planned number of scientifically relevant comparisons. The calculated significance level is reported in figure legends and main text for each experiment in the Results section. Graphs illustrate mean with S.E.M., unless otherwise stated. All given p-values are two-tailed. Linear regression analysis was used to compare individual mice, and results were reported as coefficient of determination (r²).

The results were statistically analyzed by ANOVA tests (one-way and two-way ANOVA) using Prism vs. 7 (GraphPad Software, Inc.). Statistical differences between mean values are marked with letters for the different conditions or were marked with asterisks (^*p ≤ .05; ^**p < .01; ^***p < .001; and ^****p < .0001).