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Catch and release reversible immunoprecipitation system

Manufactured by Merck Group
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Sourced in United Kingdom

The Catch and Release Reversible Immunoprecipitation System is a laboratory equipment designed for the isolation and purification of target proteins from complex samples. It utilizes a reversible binding process to capture and release proteins of interest, enabling efficient protein separation and analysis.

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Lab products found in correlation

P akt ser473, by Cell Signaling Technology (1 mentions) Total akt, by Cell Signaling Technology (1 mentions) Nampt, by Merck Group (1 mentions)

Spelling variants of this product

Catch and Release v2.0 Reversible Immunoprecipitation System (22 citations)

2 protocols using catch and release reversible immunoprecipitation system

1

Reversible Immunoprecipitation for GSK-3β and p-Tau

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The Catch and Release Reversible Immunoprecipitation System (Millipore) was used according to the manufacturer’s instructions. The proteins were eluted in 70 μL of the elution buffer and subjected to an immunoblotting analysis, using the anti-GSK-3βS9 and anti-p-TauS396 antibodies.
Cheng P.W., Wu Y.C., Wong T.Y., Sun G.C, & Tseng C.J. (2021). Mechanical Stretching-Induced Traumatic Brain Injury Is Mediated by the Formation of GSK-3β-Tau Complex to Impair Insulin Signaling Transduction. Biomedicines, 9(11), 1650.
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2

Immunoblotting and NAMPT Immunoprecipitation

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Immunoblotting was conducted as previously described [17 (link)] using primary antibodies against NAMPT (Sigma or Bethyl Laboratories, Montgomery, TX, USA), p-Akt(Ser473) and total Akt (both Cell Signaling Technologies, Danvers, MA, USA). All primary antibodies were rabbit anti-mouse polyclonal antibodies and were used at a 1:1000 dilution. Validation was conducted via immunoblot. NAMPT immunoprecipitation was carried out using a Catch and Release Reversible Immunoprecipitation System (Millipore, Watford, UK).
Kieswich J., Sayers S.R., Silvestre M.F., Harwood S.M., Yaqoob M.M, & Caton P.W. (2016). Monomeric eNAMPT in the development of experimental diabetes in mice: a potential target for type 2 diabetes treatment. Diabetologia, 59(11), 2477-2486.
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