Aria pressure volume conductance system

Manufactured by Millar

Sourced in United States

The ARIA pressure-volume conductance system is a lab equipment designed to measure the pressure-volume relationship and cardiac function in biological samples. It provides precise data on cardiac parameters such as cardiac output, stroke volume, and contractility. The system uses a conductance catheter to record the changes in ventricular volume, while also measuring the corresponding pressure changes.

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Lab products found in correlation

Pvan3, by Millar (4 mentions) Powerlab 4sp a d converter, by ADInstruments (3 mentions) Spr 839, by Millar (3 mentions) Powerlab a d converter, by ADInstruments (1 mentions) Powerlab 4sp, by ADInstruments (1 mentions) Mylab 30cv system, by Esaote (1 mentions)

6 protocols using aria pressure volume conductance system

Cardiac Function Measurement in Mice

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Cardiac function was detected by the SPR-839 instrument (Millar Instruments, Houston, TX, USA), and heart rate (HR), ejection fraction (EF), left ventricular end- systolic pressure (Pes), cardiac output (CO) were calculated as described previously [45 (link)]. Briefly, each mouse was intubated with a 22-gauge soft catheter and ventilated with a rodent ventilator (Columbus Instruments International Corp., Columbus, OH, USA), using a tidal volume of 0.3–0.5 ml and a respiratory rate of 110–120 breaths/min. After left thoracotomy, a microtip pressure-volume catheter was inserted through a 25-gauge apical stab into the left ventricular to measure the cardiac function. The signals were continuously recorded using an ARIA pressure-volume conductance system (Millar Instruments) connected to a Powerlab/4SPA/D converter (AD Instruments, Mountain View, CA, USA). All pressure-volume loop data were analyzed with PVAN3.4 (Millar Instruments). After the functional analysis, the hearts were removed and perfused for 2 min to remove the remaining blood. Portions of the midventricle were fixed for immunological studies.

Zhou Y., Song Y., Shaikh Z., Li H., Zhang H., Caudle Y., Zheng S., Yan H., Hu D., Stuart C, & Yin D. (2017). MicroRNA-155 attenuates late sepsis-induced cardiac dysfunction through JNK and β-arrestin 2. Oncotarget, 8(29), 47317-47329.

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Pressure-Volume Analysis in Anesthetized Mice

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Left ventricle pressure-volume was analyzed in mice anesthetized with 2% isoflurane by using a 1.0-F pressure-volume catheter (PVR 1045). The position of the catheter was monitored by pressure along with the magnitude and phase using the ARIA pressure-volume conductance system (Millar Instruments, Houston, Texas) and Powerlab A/D converter (AD Instruments, Mountain View, California). During this process, temperature (36.5-37.5°C) and heart rate were constantly monitored.

Li J., Wan W., Chen T., Tong S., Jiang X, & Liu W. (2019). miR-451 Silencing Inhibited Doxorubicin Exposure-Induced Cardiotoxicity in Mice. BioMed Research International, 2019, 1528278.

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In Vivo Left Ventricular Function Assessment

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In vivo LV function was assessed by Millar PV catheter as described previously [25 (link)]. Mice were anesthetized as described above and placed on heating pads with core temperature maintained at 37 °C. During the whole experiment, we monitored the vital signs (e.g. heart rate and blood pressure) under anesthesia. A microtip pressure–volume catheter (SPR-839; Millar Instruments, Houston, TX) was inserted into the right carotid artery and advanced into the left ventricle (LV) under pressure control. After stabilization for 20 min, the signals were continuously recorded at a sampling rate of 1000/s using an ARIA pressure-volume conductance system (Millar Instruments) coupled to a Powerlab/4SP analog-to-digital converter (AD Instruments, Mountain View, CA) and a personal computer. All pressure–volume loop data were analyzed using a cardiac pressure–volume analysis program (PVAN3.6; Millar Instruments), and HR, left ventricular end diastolic pressure (LVEDP), left ventricular end systolic pressure (LVESP), maximal slope of systolic pressure increment (dP/dtmax) and diastolic pressure decrement (dP/dtmin) were computed as described previously [26 (link),27 (link)].

Yang L., Ni L., Duan Q., Wang X., Chen C., Chen S., Chaugai S., Zeldin D.C., Tang J.R, & Wang D.W. (2015). CYP epoxygenase 2J2 prevents cardiac fibrosis by suppression of transmission of pro-inflammation from cardiomyocytes to macrophages. Prostaglandins & other lipid mediators, 116-117, 64-75.

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Evaluating Cardiac Function Using SPR-839 Instrument

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Cardiac function was detected by use of the SPR-839 instrument (Millar Instruments, Houston, TX, USA) as described previously by us [21] (link). Briefly, a microtip pressure–volume catheter (SPR-839; Millar Instruments, Houston, TX, USA) was inserted through a 25-gauge apical stab into the LV to measure the steady-state cardiac function. At the completion of the study, 10 µL of hypertonic saline (15%) was injected into the right atrium to calibrate Vp, the parallel volume. The signals were continuously recorded at a sampling rate of 1000 s⁻¹ using an ARIA pressure–volume conductance system (Millar Instruments) coupled to a Powerlab/4SP A/D converter (AD Instruments, Mountain View, CA, USA). All pressure–volume loop data were analyzed with a cardiac pressure–volume analysis program (PVAN3.4; Millar Instruments). At the end of the functional analysis, the hearts were removed and perfused for 2 min as Langendorff preparations to remove the remaining blood before Western blot analysis.

Yan H., Li H., Denney J., Daniels C., Singh K., Chua B., Stuart C., Caudle Y., Hamdy R., LeSage G, & Yin D. (2016). β-arrestin 2 attenuates cardiac dysfunction in polymicrobial sepsis through gp130 and p38. Biochemistry and Biophysics Reports, 7, 130-137.

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Cardiac Function Analysis via Pressure-Volume Catheter

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Cardiac function was detected by use of the SPR-839 instrument (Millar Instruments, Houston, TX, USA) as described previously by us [21 (link)]. Briefly, a microtip pressure–volume catheter (SPR-839; Millar Instruments, Houston, TX, USA) was inserted through a 25-gauge apical stab into the LV to measure the steady-state cardiac function. At the completion of the study, 10 μL of hypertonic saline (15%) was injected into the right atrium to calibrate Vp, the parallel volume. The signals were continuously recorded at a sampling rate of 1000 s–1 using an ARIA pressure–volume conductance system (Millar Instruments) coupled to a Powerlab/4SP A/D converter (AD Instruments, Mountain View, CA, USA). All pressure–volume loop data were analyzed with a cardiac pressure–volume analysis program (PVAN3.4; Millar Instruments). At the end of the functional analysis, the hearts were removed and perfused for 2 min as Langendorff preparations to remove the remaining blood before Western blot analysis.

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Cardiac Function Assessment in Mice

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At 4 weeks after surgery, the mice were anaesthetised by inhalation of 1.5–2% isoflurane. Echocardiography was performed to evaluate the function of the left ventricle using a MyLab 30CV system (Biosound Esaote, Inc.) equipped with a 15-MHz probe. M-mode tracings derived from the short axis of the left ventricle at the level of the papillary muscles were recorded. For haemodynamic analysis, insertion of a 1.4-French catheter-tip micromanometer catheter (Millar Instruments) into the left ventricle via the right carotid artery was performed. The heart rates, pressure and volume signals were continuously recorded using an Aria pressure-volume conductance system (Millar Instruments) coupled with a PowerLab/4SP A/D converter. According to the guidelines of the Chinese Animal Welfare Committee, subsequent to pressure-volume measurement, the mice were anaesthetised with 1.5% pentobarbital sodium (60 mg/kg) and then sacrificed by cervical dislocation under anaesthesia.

Wang Z.P., Che Y., Zhou H., Meng Y.Y., Wu H.M., Jin Y.G., Wu Q.Q., Wang S.S, & Yuan Y. (2020). Corosolic acid attenuates cardiac fibrosis following myocardial infarction in mice. International Journal of Molecular Medicine, 45(5), 1425-1435.

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