The following compounds were used for myograph studies and purchased from Sigma-Aldrich (St. Louis, MO, USA): (R)-(−)-phenylephrine hydrochloride (PE), acetylcholine chloride (ACh), adenosine 5′ diphosphate sodium salt (ADP), potassium phosphate monobasic, potassium chloride, D-(+)-glucose, magnesium sulfate heptahydrate, sodium chloride, calcium chloride dehydrate, α,ß-methylene adenosine 5′-triphosphate (α,ß-MetATP), guanethidine, Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME). DEA NONOate and NF449 were purchased from Cayman Chemicals (Ann Arbor, MI, USA), and MRS2500, 2-methylthioadenosine diphosphate trisodium salt (2-MeSADP), and tetrodotoxin citrate from Tocris (Bristol, United Kingdom). All compounds were dissolved in distilled water with the exception of atropine and DEA NONOate which were dissolved in 100% ethanol and further diluted using distilled water.
2 methylthioadenosine diphosphate trisodium salt 2 mesadp
Manufactured by Bio-Techne
Sourced in United States, Germany
2-methylthioadenosine diphosphate trisodium salt (2-MeSADP) is a chemical compound that can be used as a research tool. It is a potent and selective P2Y1 receptor agonist.
Automatically generated - may contain errors
Lab products found in correlation
Acetylcholine chloride, by Merck Group (1 mentions)
Nω nitro l arginine methyl ester hydrochloride l name, by Merck Group (1 mentions)
Tetrodotoxin citrate, by Bio-Techne (1 mentions)
Nf449, by Cayman Chemical (1 mentions)
Adenosine 5 diphosphate sodium salt, by Merck Group (1 mentions)
R phenylephrine hydrochloride pe, by Merck Group (1 mentions)
Dea nonoate, by Cayman Chemical (1 mentions)
Mrs2500, by Bio-Techne (1 mentions)
D glucose, by Merck Group (1 mentions)
Potassium phosphate monobasic, by Merck Group (1 mentions)
Dipyridamole, by Merck Group (1 mentions)
Adenosine, by Merck Group (1 mentions)
Sucrose, by Carl Roth (1 mentions)
Ticagrelor, by Cayman Chemical (1 mentions)
D glucose, by Carl Roth (1 mentions)
Tween 20, by Carl Roth (1 mentions)
Apyrase, by Merck Group (1 mentions)
Cacl2, by Carl Roth (1 mentions)
K2hpo4, by Carl Roth (1 mentions)
Nahco3, by Carl Roth (1 mentions)
2 protocols using 2 methylthioadenosine diphosphate trisodium salt 2 mesadp
1
Vascular Reactivity Assays Using Myograph
2
Reagents for Platelet Activation Assays
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ATP, ADP, AMP, adenosine, dipyridamole, apyrase and dimethylsulfoxid (DMSO) were purchased from Sigma (Taufkirchen, Germany); 2-methylthioadenosine diphosphate trisodium salt (2meSADP) was from Tocris (Bristol, UK); DMEM, Dulbecco’s Phosphate-Buffered saline without Ca2+ and Mg2+, trypsin/EDTA solution, HEPES was obtained from Thermo Scientific (Walldorf, Germany); NaCl, KCl, MgCl2, CaCl2, K2HPO4, NaHCO3, D-glucose, paraformaldehyde (PFA), Triton X-100, Tween 20, bovine serum albumin (BSA), and sucrose were from Roth (Karlsruhe, Germany). Heparin (Heparin-Natrium-250 000-ratiopharm) was from Ratiopharm (Ulm, Germany), Narcorene® from Merial GmbH (Hallbergmoos, Germany), ticagrelor was purchased from Cayman Chemical (Hamburg, Germany); concentrated stock solutions of ticagrelor and dipyridamole were prepared in DMSO and diluted in artificial cerebrospinal fluid (ACSF) or cell culture medium right before use.
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