Vs120 l100 w virtual slide microscope

Following the EEG recording session, rats were deeply anesthetized with isoflurane (diisopropyl fluorophosphate model) or sodium pentobarbital (soman model). They were then transcardially perfused with saline until fully exsanguinated, followed by 10% formalin for tissue fixation. For the diisopropyl fluorophosphate model, brains were subsequently placed in 30% sucrose for cryoprotection. Brains were then flash frozen and 30 μm coronal sections were cut between bregma coordinates −1.8 to −6.3 mm (Paxinos and Watson, 2006 ). For the soman model, brains were embedded in paraffin and sectioned into 5 μm coronal slices. The section corresponding to 3.24 mm posterior to bregma (Paxinos and Watson, 2006 ) was collected. All brain sections were mounted on glass slides and stained with FluoroJade B (Histo-chem, Inc., Jefferson, AR, United States) using previously described techniques (Schmued and Hopkins, 2000 (link)). Individual sections were imaged with a Hamamatsu Nanozoomer 2.0 HT (diisopropyl fluorophosphate model) or an Olympus VS120-L100-W virtual slide microscope (soman model) and VS-ASW software (Olympus Corporation, Tokyo, Japan).