At specific time points before, during and after inoculation of mice with live P. gingivalis, peripheral blood was drawn and stained with the following antibodies: Alexafluor 700 conjugated anti-mouse CD3ε, Peridinin Clorophyll/Cyanine 5.5 conjugated anti-mouse CD45, Pacific Blue conjugated anti mouse CD4 (all BD Biosciences) prior to acquisition on a SORP 5-Laser LSR II (BD Immunocytometry systems). Analysis was performed using FlowJo v10.5.
Sorp 5 laser lsr 2
Manufactured by BD
The SORP 5-Laser LSR II is a flow cytometer designed for advanced multiparametric analysis. It features five lasers and multiple detectors, enabling the simultaneous measurement of multiple cellular parameters. The core function of the SORP 5-Laser LSR II is to perform high-sensitivity, high-resolution flow cytometry analysis.
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2 protocols using sorp 5 laser lsr 2
1
Peripheral Blood Immune Profiling in P. gingivalis Infected Mice
2
Analyzing T-cell Subsets in P. gingivalis Infection
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At specific time points before, during, and after brushing with live P. gingivalis, peripheral blood was drawn and stained with the following antibodies: Alexafluor 700 conjugated anti-mouse CD3ε, Peridinin Clorophyll/Cyanine 5.5 conjugated anti-mouse CD45, Pacific Blue conjugated anti-mouse CD4, and Phycoerythrin/Cyanine 7 conjugated anti-mouse CD25 (all BD Biosciences) prior to acquisition on a SORP 5-Laser LSR II (BD Immunocytometry Systems). In some experiments, cervical lymph nodes were obtained at the time of sacrifice and were subjected to the same panel of antibodies. Analysis was performed using FlowJo v10.1.
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