Total RNA (30μg) was separated by 1% agarose formaldehyde gel electrophoresis and transferred overnight to a nylon membrane (Hybond-N, GE Healthcare) by the capillary method. RNA was cross-linked to the membrane with UV and hybridized to 0.1nM double-DIG labeled antisense miRCURY LNA probes against miR-193b (Exiqon). The membrane was probed with anti-DIG AP antibody (Roche, Cat#11093274910) and detected using chemiluminescence (Roche CDP-Star, Cat# 12041677001) which was imaged using a SyngeneG:Box imaging system.
Mircury lna probes against mir 193b
Manufactured by Qiagen
The MiRCURY LNA probes against miR-193b are a set of nucleic acid probes designed to detect and quantify the expression of miR-193b, a microRNA molecule involved in various biological processes. These probes utilize Locked Nucleic Acid (LNA) technology to enhance their specificity and sensitivity in detecting the target miR-193b.
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Lab products found in correlation
2 protocols using mircury lna probes against mir 193b
1
Quantitative miRNA Northern Blot Analysis
2
Quantitative miRNA Northern Blot Analysis
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Total RNA (30μg) was separated by 1% agarose formaldehyde gel electrophoresis and transferred overnight to a nylon membrane (Hybond-N, GE Healthcare) by the capillary method. RNA was cross-linked to the membrane with UV and hybridized to 0.1nM double-DIG labeled antisense miRCURY LNA probes against miR-193b (Exiqon). The membrane was probed with anti-DIG AP antibody (Roche, Cat#11093274910) and detected using chemiluminescence (Roche CDP-Star, Cat# 12041677001) which was imaged using a SyngeneG:Box imaging system.
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