Total protein of DU145 and PC3 cells was extracted with 2× loading buffer (Solarbio, Beijing, China). Antibodies used in western blotting are shown below: GAPDH (1:50000, 60004-1-Ig, Proteintech), c-Myc (1:1000, ab32072, Abcam), cyclin D1 (1:20000, ab134175, Abcam), Akt (1:2000, 4691, CST), p-Akt (1:2000, 4060, CST), FGFR2 (1:500, ab289968, Abcam), HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H + L) and Peroxidase-conjugated Affinipure Goat Anti-Mouse IgG(H + L) (1:5000, SA00001-2 and SA00001-1, Proteintech). The blots were quantified by Image J software. Uncropped scans can be found in Figure S1.
Peroxidase conjugated affinipure goat anti mouse igg
Manufactured by Proteintech
Peroxidase-conjugated Affinipure Goat Anti-Mouse IgG is a secondary antibody that is conjugated to the enzyme peroxidase. It is designed to detect and bind to mouse immunoglobulin G (IgG) antibodies in various immunoassays and detection methods.
Automatically generated - may contain errors
Lab products found in correlation
Ab134175, by Abcam (1 mentions)
Sa00001 1, by Proteintech (1 mentions)
Sa00001 2, by Proteintech (1 mentions)
Ab32072, by Abcam (1 mentions)
Polyattract mrna isolation system, by Promega (1 mentions)
Pcr select cdna subtraction kit, by Takara Bio (1 mentions)
Peroxidase conjugated affinipure goat anti rabbit igg, by Proteintech (1 mentions)
Anti β actin, by Proteintech (1 mentions)
X tremegene sirna transfection reagent, by Roche (1 mentions)
X tremegene hp dna transfection reagent, by Roche (1 mentions)
2 protocols using peroxidase conjugated affinipure goat anti mouse igg
1
Protein Expression Analysis in Prostate Cancer
2
Cyclophilin B Antibody Reagents and Techniques
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The antibodies used in this study were anti-Cyclophilin B polyconal rabbit antibody (Abcam), PE-Cy5 conjugated rabbit anti-Cyclophilin B antibody (Bioss), anti-β-actin (Proteintech), Peroxidase-conjugated Affinipure Goat Anti-Mouse IgG (Proteintech) and Peroxidase-conjugated Affinipure Goat Anti-Rabbit IgG (Proteintech). CsA were purchased from Sigma. PolyATtract® mRNA Isolation Systems was purchased from Promega. PCR-Select™ cDNA Subtraction Kit and Advantage cDNA PCR Kit & Polymerase Mix were obtained from Clontech. X-treme GENE HP DNA Transfection Reagent and X-treme GENE siRNA Transfection Reagent were purchased from Roche.
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