Phenylephrine HCl, Acetylcholine chloride, Prazosin HCl, and Sodium nitroprusside dihydrate were all obtained from Sigma Chemical Company (St. Louis, MO, USA). Sildenafil was bought from the government pharmaceutical organization (GPO) of Thailand (Bangkok, Thailand). DMSO was acquired from VWR International Ltd. (Prolabo Chemicals, UK). All quinazoline analogues were dissolved in DMSO (100%) and diluted with distilled water.
Sodium nitroprusside dihydrate
Manufactured by Merck Group
Sourced in United States, Germany
Sodium nitroprusside dihydrate is a chemical compound used as a laboratory reagent. It is a crystalline solid that is soluble in water and forms a dark red solution. Sodium nitroprusside dihydrate is commonly used in analytical and biochemical applications, but its specific functions and uses should not be extrapolated or interpreted beyond providing this factual description.
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Lab products found in correlation
Acetylcholine chloride, by Merck Group (3 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (2 mentions)
Ethylenediaminetetraacetic acid, by Merck Group (2 mentions)
Dimethyl sulfoxide (dmso), by Avantor (1 mentions)
Phenylephrine hcl, by Merck Group (1 mentions)
Prazosin hcl, by Merck Group (1 mentions)
Bovine insulin, by Merck Group (1 mentions)
Reduced glutathione ethyl ester, by Merck Group (1 mentions)
Cytochalasin b, by Merck Group (1 mentions)
Angiotensin 2, by Merck Group (1 mentions)
Diamide, by Merck Group (1 mentions)
Recombinant human insulin, by Merck Group (1 mentions)
Diethyl maleate, by Merck Group (1 mentions)
S nitroso n acetylpenicillamine, by Merck Group (1 mentions)
Superoxide dismutase sod, by Merck Group (1 mentions)
Epinephrine hydrochloride, by Merck Group (1 mentions)
Aminoguanidine hemisulfate salt, by Merck Group (1 mentions)
S methylisothiourea hemisulfate salt, by Merck Group (1 mentions)
L ng nitroarginine l nna, by Cayman Chemical (1 mentions)
12 protocols using sodium nitroprusside dihydrate
1
Vasoactive Compounds: Preparation and Testing
2
Insulin Signaling and Glucose Uptake Assay
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The hydrogen peroxide solution (30 wt% in water), S-nitroso-N-acetylpenicillamine (SNAP), Sodium nitroprusside dihydrate (SNP), NOC-7, Diamide, Diethyl Maleate (DEM), Glutathione reduced ethyl ester (GSHEE), Superoxide dismutase (SOD), Angiotensin II, Cytochalasin B, bovine insulin, and recombinant human insulin were obtained from Merck-Sigma-Aldrich (Darmstadt, Germany). Insulin receptor antagonist peptide (S961) was obtained from Phoenix Pharmaceuticals, Inc. (Burlingame, CA, USA). 6-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl) amino)-6-Deoxyglucose (6-NBDG) was obtained from Molecular ProbesTM, Invitrogen, Thermo Fisher Scientific (Waltham, MA, USA).
3
Marine organism chemical treatment protocol
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The catecholamine epinephrine hydrochloride (EPI), the NOS inhibitors S-methylisothiourea hemisulfate salt (SMIS) and aminoguanidine hemisulfate salt (AGH) as well as the NO donor sodium nitroprusside dihydrate (SNP) were purchased from Sigma-Aldrich. The irreversible inhibitor of soluble guanylyl cyclase 1H-[1,2,4]Oxadiazole[4,3-a]quinoxalin-1-one (ODQ) and the NO donor 3-morpholinosydnonimine chloride (SIN-1) were obtained from AdipoGen Life Sciences and the NOS inhibitors L-NG-nitroarginine methyl ester (L-NAME) hydrochloride, L-NG-nitroarginine (L-NNA) and 7-nitroindazole (7-NI) were purchased from Cayman chemicals. Additional chemicals were Levodopa (L-DOPA), obtained from Santa Cruz Biotechnology, as well as (+)-MK 801 maleate (MK-801) and Ifenprodil (+)-tartrate salt (ifenprodil), both obtained from Selleckchem. Stock solutions at 10− 1 M for SMIS, AGH and SNP or at 10− 2 M for remaining compounds were either prepared with autoclaved Milli-Q dH2O or with DMSO (Sigma-Aldrich) for 7-NI and ODQ. Working solutions (10x concentrate of final concentration of treatment) for each compound were prepared prior to experiments with 1 μm filtered fresh seawater (FSW).
4
Cytotoxicity and ROS Evaluation of SNP
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Sodium nitroprusside dihydrate (Na2[Fe(CN)5NO]·2H2O, SNP, ≥99%) (SNP), silver nitrate (AgNO3), and anhydrous methanol (99.8%) were purchased from Sigma-Aldrich (St. Louis, USA). A Millipore Milli-Q Biocell A10 water purifying system was used to prepare ultrapure water. A2780, A2780cis, U-87 MG (Sigma-Aldrich, St. Louis, MO, USA), MDA-MB-231 (Cell Biolabs, CA, USA) SK-OV-3, MCF-7, MRC-5, W1-38 (ATCC, Manassas, VA, USA) cells were cultured according to the manufacturers’ instruction. LysoTracker Green DND-26, Hoechst 33342, and rhodamine B were purchased from (ThermoFisher Scientific, Waltham, MA, USA). CellTiter-Glo® from (Promega, Madison, WI, USA), PE-Annexin V Apoptosis Detection Kit from (Becton-Dickinson Franklin Lakes, NJ, USA). ROS-Glo™ H2O2 luminescence assay from (Promega, Madison, WI, USA), DAX-J2 PON Green Kit was purchased from (AAT Bioquest, Sunnyvale, CA, USA), FluorSave™ reagent (Millipore, Burlington, MA, USA). qPCR Primers from (Integrated DNA Technologies, Bologna, Italy).
5
Cocaine, Testosterone, and Antibiotic Protocol
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Cocaine hydrochloride (Sigma, St Louis, MO, USA), phenylephrine hydrochloride (Sigma), sodium nitroprusside dihydrate (Sigma), and tribromoethanol (Sigma) were dissolved in saline (0.9% NaCl). Testosterone propionate (PharmaNostra, Rio de Janeiro, RJ, Brazil) was dissolved in almond oil. Flunixine meglumine (Banamine, Schering-Plough, Cotia, SP, Brazil) and the poly-antibiotic preparation (Pentabiotico, Fort Dodge, Campinas, SP, Brazil) were used as provided.
6
Evaluation of Pharmaceutical Compounds
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Sodium nitroprusside dihydrate (SNP) and fluorescein isothiocyanate-dextran 4,000 (FD-4) were obtained from Sigma-Aldrich Co. Ltd. (St. Louis, MO, USA). Carboxy-PTIO (c-PTIO) was obtained from Dojindo Laboratories (Kumamoto, Japan). Griseofulvin, antipyrine, theophylline, and sodium ferrocyanide decahydrate (SFC) were obtained from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Flurbiprofen was obtained from LKT Laboratories (St. Paul, MN, USA). Propranolol was obtained from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). All other reagents were of analytical reagent grade.
7
Biochemical Reagents in Research
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Acetylcholine chloride, sodium nitroprusside dihydrate, and thromboxane A2 analog were purchased from Sigma-Aldrich Co. (Saint Louis, USA).
8
Antioxidant Capacity Evaluation Protocol
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Folin-Ciocalteu reagent, ABTS (2,2'-azino-bis-3--ethylbenzothiazoline-6-sulfonic acid), DPPH (1,1-diphenyl-2picrylhydrazyl), tannic acid, catechin, L-ascorbic acid, DAF-2 (4,5-Diamino fluroprusside), Sodium nitroprusside dihydrate, etc. are Sigma-Aldrich Co. (St. Louis, MO, USA) products were used, and other extraction solvents and all reagents used were special reagents. A spectrophotometer (Neogen, Optizen 2120 UV, Sejong, Korea), ELISA reader (Thermo Fisher SCIENTIFIC, Multiskan Sky, KOREA) was used as the instruent.
9
Bovine Lactoferrin Hydrolysis Protocol
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Bovine LF was provided by FrieslandCampina Domo (Zwolle, The Netherlands). LF was hydrolysed using porcine pepsin (Sigma-Aldrich, Madrid, Spain) and the product was subjected to ultrafiltration through a polyethersulfone membrane with a 3 kDa cut-off (Vivascience, Sartorius Stedim Biotech, Aubagne, France) as previously described [15] (link). Protein content of the permeate LFH was estimated by the bicinchoninic acid method using bovine serum albumin (Sigma-Aldrich) as standard. LFH was freeze-dried and kept at -20 °C until use. LF-derived peptide of sequence DPYKLRP [17] (link) was purchased at >95% purity from GenScript Corporation (Piscataway, NJ, USA), wherein it was synthesized by solid phase methods using N-(9-fluorenyl) methoxycarbonyl (Fmoc) chemistry. Heptapeptide concentration was based on the dry weight.
Acetylcholine chloride (Ach), indomethacin (IND), Nω-nitro-L-arginine methyl ester hydrochloride (L-NAME), L-phenylephrine hydrochloride (PE) and sodium nitroprusside dihydrate (SNP) were obtained from Sigma-Aldrich.
The Ringer-Locke solution had the following composition (mM): NaCl 120, KCl 5.4, CaCl 2 2.2, MgCl 2 1.0, NaHCO 3 25 and glucose 5.6. In 120 mM KCl-depolarizing solution, NaCl was replaced by an equimolar amount of KCl.
Acetylcholine chloride (Ach), indomethacin (IND), Nω-nitro-L-arginine methyl ester hydrochloride (L-NAME), L-phenylephrine hydrochloride (PE) and sodium nitroprusside dihydrate (SNP) were obtained from Sigma-Aldrich.
The Ringer-Locke solution had the following composition (mM): NaCl 120, KCl 5.4, CaCl 2 2.2, MgCl 2 1.0, NaHCO 3 25 and glucose 5.6. In 120 mM KCl-depolarizing solution, NaCl was replaced by an equimolar amount of KCl.
10
Preparation of Pharmacological Solutions
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Acetylcholine chloride (Miochol-E, Novartis Healthcare, Denmark) and sodium chloride, 9 mg/mL (Baxter Medical AB, Kista, Sweden), were obtained from the hospital pharmacy.
Sodium nitroprusside dihydrate and caffeine were obtained from Sigma Aldrich (St. Louis, MO, USA). Drugs were dissolved in sterile water to a final concentration of 10 mg/mL. All drug solutions were prepared on the day of the experiments.
Sodium nitroprusside dihydrate and caffeine were obtained from Sigma Aldrich (St. Louis, MO, USA). Drugs were dissolved in sterile water to a final concentration of 10 mg/mL. All drug solutions were prepared on the day of the experiments.
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