Cd19 j3 119

Manufactured by Beckman Coulter

Sourced in United States

The CD19 (J3-119) is a lab equipment product from Beckman Coulter. It is a monoclonal antibody that recognizes the CD19 antigen, which is expressed on the surface of B cells.

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CD19-ECD (J3-119) (7 citations) J3-119 (5 citations) PE-Cy5 anti-CD19 (J3-119) (3 citations) CD19-PC7 (clone J3-119, (3 citations) CD19-PE-Cy7 (clone J3-119; (3 citations) Anti-CD19 (J3-119) (2 citations) Anti-human CD19-Pc7 (clone J3-119; (2 citations)

4 protocols using cd19 j3 119

Multiparameter Flow Cytometry of Macaque Immune Cells

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The following fluorochrome conjugated monoclonal antibodies reactive with macaque cells were used for flow cytometry studies: CD3 (SP34-2, BD Biosciences [BD]), CD4 (L200, BD), CD8 (SK1, BD), CD95 (DX2, BD), CD28 (CD28.2, BioLegend), CCR5 (3A9, BD), CXCR5 (MU5UBEE, Invitrogen), PD-1 (EH12.2H7, BioLegend), ICOS (C398.4A, BioLegend), CCR7 (150503, BD), α4β7 (A4B7, NHP Reagent Resource), LAG3 (3DS223H, eBioscience), Tim-3 (F38-2E2, BioLegend), TIGIT (MBSA43, Invitrogen), CD20 (2H7, BioLegend), CD19 (J3-119, Beckman Coulter), HLA-DR (L243, BioLegend), CD10 (HI10A, BioLegend), CD21 (B-ly4, BD), CD27 (O323, BioLegend), IgD (IADB6, Southern Biotech), IgG (G18-145, BD), IL-21R (2G1-K12, BioLegend), Ki-67 (B56, BD), BCL6 (IG191E/A8, BioLegend), CD80 (2D10, BioLegend), CD56 (B159, BD), CD45 (D058-1283, BD), CD14 (MoP9, BD), CD16 (3G8, BD), CCR2 (48607, R&D Systems), CD11b (ICRF44, BioLegend), CD11c (3.9, Invitrogen), PD-L1 (29E.2A3, BioLegend), PD-L2 (24F.10C12, BioLegend), CD80 (2D10, BioLegend), CD86 (FUN-1, BD), CD141 (1A4, BD), CD163 (GHI/61, BioLegend), and CD123 (7G3, BD).

Kvistad D., Pallikkuth S., Sirupangi T., Pahwa R., Kizhner A., Petrovas C., Villinger F, & Pahwa S. (2021). IL-21 enhances influenza vaccine responses in aged macaques with suppressed SIV infection. JCI Insight, 6(20), e150888.

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Multiparametric Flow Cytometry Analysis of Immune Cells

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PBMCs were isolated from heparinized blood samples by using the Ficoll Isopaque density gradient centrifugation (Amersham Pharmacia Biotech, Uppsala, Sweden), and viably frozen and stored in liquid nitrogen until further processing. The flow cytometry staining protocol is described elsewhere 57.
Antibodies against human CD5 (L17F12), CD8 (Sk‐1), CD21 (B‐Ly4), CD31 (WM59), CD38 (HIT2), KI‐67 (B56), PD‐1 (CD279, Clone MIH4), CXCR5 (RF8B2) were from BD Biosciences (San Jose, CA), Goat F(ab’)2 IgM and IgG from Southern Biotech(Birmingham, AL), CD19 (J3‐119) from Beckman Coulter (Fullerton, CA), CD10 (eBioCB‐CALLA), Foxp3 (PCH101) from eBiosciences (San Diego, CA), CD25 (BC96), CD127 (HCD127) from Sony biotechnology, (San Jose, CA) and CD3 (UCHT1), CD4 (RPA‐T4), CD27 (O323), CD45RO (UCHL1), CD45RA (HI100), IL‐21 (3A3‐N2) from Biolegend (San Diego, CA). Finally, stained mononuclear cells were washed twice in FACS buffer and run on an FACS Canto II and analyzed by using FlowJo software (Treestar). Naïve T cells were defined as CD3⁺CD45RA⁺CCR7⁺, memory T cells as CD3⁺ CD45RO⁺, Treg cells as CD3⁺CD4⁺Foxp3⁺, and Tfh as CD3⁺CD4⁺CXCR5⁺PD1⁺

van den Broek T., Madi A., Delemarre E.M., Schadenberg A.W., Tesselaar K., Borghans J.A., Nierkens S., Redegeld F.A., Otten H.G., Rossetti M., Albani S., Sorek R., Cohen I.R., Jansen N.J, & van Wijk F. (2017). Human neonatal thymectomy induces altered B‐cell responses and autoreactivity. European Journal of Immunology, 47(11), 1970-1981.

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Flow Cytometric Analysis of STLV-1 Infection

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Bone marrow mononuclear cells from two STLV-1 infected JMs and an uninfected JM were prepared by density gradient centrifugation using Ficoll-Paque PLUS (GE Healthcare Bio-Science). CD8 T lymphocytes were removed by positive selection with BD IMag (BD Bioscience). CD8 T lymphocytes depleted cells were cultured for 24 hours with RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) and antibiotics. The following antibody was used for cell surface staining: anti-CD4 (OKT4), CD34 (561) (all from BioLegend), CD8 (RPA-T8), CD3 (SP34-2), CD14 (M5E2), (all from BD Bioscience), CD19 (J3-119) (from Beckman Coulter), CD33 (AC104.3E3) (from Miltenyi Biotec). After cell surface staining, cells were fixed and permeabilized with Fixation/Permeabilization working solution (eBioscience), and Tax was stained by anti-Tax monoclonal antibody (MI73)(39). Samples were analyzed on a FACSVerse with FACSuite software (BD Biosciences) and data was analyzed with Flow Jo software (FlowJo, LLC).

Furuta R., Yasunaga J.I., Miura M., Sugata K., Saito A., Akari H., Ueno T., Takenouchi N., Fujisawa J.I., Koh K.R., Higuchi Y., Mahgoub M., Shimizu M., Matsuda F., Melamed A., Bangham C.R, & Matsuoka M. (2017). Human T-cell leukemia virus type 1 infects multiple lineage hematopoietic cells in vivo. PLoS Pathogens, 13(11), e1006722.

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Characterization of Mesenchymal Stem Cells by Flow Cytometry

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Trypsinized MSCs from P2 to P10 were immunophenotypically characterized by flow cytometry using anti-human monoclonal antibodies (mAb) against CD29 (4B4; Cyto-Stat/Beckman Coulter, Fullerton, CA, USA), CD44 (J173; Immunotech/Beckman Coulter, Marseille, France), CD73 (AD2; BD Biosciences Pharmingen, San Diego, CA, USA), CD90 (F15.42; Immunotech/ Beckman Coulter), CD105 (SN6; Caltag, Burlingame, CA, USA), anti-CD146 (P1H12; BD Biosciences Pharmingen), CD45 (IMMU19.2; Immunotech/ Beckman Coulter), CD14 (RMO52; Immunotech/Beckman Coulter), CD34 (QBend10; Beckman Coulter), CD31 (5.6E; Immunotech/Beckman Coulter), CD19 (J3-119; Immunotech/Beckman Coulter) and HLA-DR (Immu-357, Immunotech/Beckman Coulter), as previously described [6 (link)].
Flow cytometry was also used to study the apoptotic characteristics of MSCs at two representative passages (P2 and P6) by means of 7-aminoactinomycin D staining (7-AAD; Calbiochem-Novabiochem, Nottingham, UK) as previously described [9 (link)]. Results were expressed as proportions of 7-AAD^neg (live), 7-AAD^dim (early apoptotic) and 7-AAD^bright (late apoptotic) cells. Acquisition and analysis were performed in a Cytomics FC 500 flow cytometer (Beckman Coulter, Brea, CA, USA) on a minimum of 10,000 events.

Batsali A.K., Pontikoglou C., Koutroulakis D., Pavlaki K.I., Damianaki A., Mavroudi I., Alpantaki K., Kouvidi E., Kontakis G, & Papadaki H.A. (2017). Differential expression of cell cycle and WNT pathway-related genes accounts for differences in the growth and differentiation potential of Wharton’s jelly and bone marrow-derived mesenchymal stem cells. Stem Cell Research & Therapy, 8, 102.

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