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Apo alert annexin 5 kit

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The Apo-Alert Annexin V kit is a laboratory reagent used for the detection and quantification of apoptosis, a type of programmed cell death. The kit utilizes Annexin V, a calcium-dependent phospholipid-binding protein, to label cells undergoing apoptosis. This allows for the identification and analysis of apoptotic cells using flow cytometry or fluorescence microscopy.

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Lab products found in correlation

Facsarray bioanalyzer, by BD (2 mentions) Cell cycle detection kit, by Beyotime (1 mentions)

3 protocols using apo alert annexin 5 kit

1

Apoptosis Assay of NaHS-Treated Cells

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Cells were seeded into 35 mm dishes (1 × 105 cells/dish) and treated with NaHS, or PBS. The cells were fixed and analyzed by flow cytometry (FACS) (BD FACS Array Bioanalyzer). Apoptotic cells were stained using the Apo-Alert Annexin V kit (BD Biosciences) before analysis.
Wang R., Fan Q., Zhang J., Zhang X., Kang Y, & Wang Z. (2018). Hydrogen Sulfide Demonstrates Promising Antitumor Efficacy in Gastric Carcinoma by Targeting MGAT5. Translational Oncology, 11(4), 900-910.
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2

Annexin-V Apoptosis Assay Protocol

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Cells were initially seeded into 12-well plates with 1 × 105 per well and incubated with DMSO, BFA and GCA with proper concentrations and times. Annexin-V assay was performed on flow cytometry (BD Biosciences) according to the manufacturer’s instruction provided by the ApoAlert Annexin-V kit (BD Clontech). Here, cell death was determined by positive staining of Annexin-V and propidium iodide (PI) alone, or both.
Shen S.M., Ji Y., Zhang C., Dong S.S., Yang S., Xiong Z., Ge M.K., Yu Y., Xia L., Guo M., Cheng J.K., Liu J.L., Yu J.X, & Chen G.Q. (2018). Nuclear PTEN safeguards pre-mRNA splicing to link Golgi apparatus for its tumor suppressive role. Nature Communications, 9, 2392.
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3

Cell Cycle and Apoptosis Analysis

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Cells were seeded into 10-cm dishes (1 × 106 cells/dish) and treated with DATS and/or DOC. The cells were fixed and stained in a solution containing propidium iodide (PI) overnight at 4°C, following the instructions of the cell cycle detection kit (Beyotime Ltd.), and then analyzed by flow cytometry (BD FACS Array™ Bioanalyzer; BD Biosciences). Apoptotic cells were stained using the Apo-Alert Annexin V kit (BD Biosciences) before analysis.
Pan Y., Lin S., Xing R., Zhu M., Lin B., Cui J., Li W., Gao J., Shen L., Zhao Y., Guo M., Wang J.M., Huang J, & Lu Y. (2016). Epigenetic Upregulation of Metallothionein 2A by Diallyl Trisulfide Enhances Chemosensitivity of Human Gastric Cancer Cells to Docetaxel Through Attenuating NF-κB Activation. Antioxidants & Redox Signaling, 24(15), 839-854.
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