1010 tem

Manufactured by JEOL

Sourced in Japan, United States

The JEOL 1010 TEM is a transmission electron microscope (TEM) designed for high-resolution imaging and analysis of small-scale samples. The TEM uses a focused electron beam to produce magnified images of the internal structure and composition of materials at the nanometer scale. The JEOL 1010 TEM provides a core function of enabling detailed observation and study of a wide range of specimens, including biological samples, thin films, and nanomaterials.

Automatically generated - may contain errors

Lab products found in correlation

Ultracut uct microtome, by Leica (3 mentions) Nanogold 1.4 nm, by Nanoprobes (2 mentions) 1010 transmission electron microscope, by JEOL (2 mentions) Ccd camera, by Ametek (1 mentions) Branson 2510 ultrasonic cleaner, by Emerson (1 mentions) Malvern zetasizer nano z, by Malvern Panalytical (1 mentions) 1400plus microscope, by JEOL (1 mentions) Ultracut r, by Leica (1 mentions) Dfc450 c camera, by Leica (1 mentions) Megaview 3 camera, by JEOL (1 mentions) 2010 tem, by JEOL (1 mentions) Uranyl acetate, by Merck Group (1 mentions) S 4700, by Hitachi (1 mentions) Axioskop 40, by Zeiss (1 mentions) Jfc 1100, by JEOL (1 mentions) Ultracut s, by Leica (1 mentions) Vt1000, by Leica (1 mentions) Oriussc100b charge coupled device camera, by Ametek (1 mentions) Morada ccd camera, by Olympus (1 mentions) Item software, by Olympus (1 mentions)

52 protocols using 1010 tem

Ultrastructural and Immunogold Electron Microscopy

Check if the same lab product or an alternative is used in the 5 most similar protocols

After removal of culture medium, UM-SCC-22A cells were fixed in 2% (w/v) glutaraldehyde in 0.1 M cacodylate buffer. After post-fixation in 2% (v/v) osmium tetroxide, specimens were embedded in Epon 812, and sections were cut orthogonally to the cell monolayer with a diamond knife. Thin sections were visualized in a JEOL 1010 TEM. In immunoelectron microscopy studies, after fixing with 4% paraformaldehyde (PFA), cells were permeabilized with 0.1% Triton X-100 for 10 min at room temperature (RT), washed, and blocked with 1% BSA in PBS for 20 min. Corresponding primary antibodies (1:50 dilution) were added to cells and incubated overnight at 4°C. After washing with PBS, Nanogold (1.4 nm) (Nanoprobes)–conjugated anti-mouse or anti-rat Fab fragments (1:200) were incubated with cells for 1 hour at RT. After post-fixation with 1% glutaraldehyde in PBS for 10 min at RT, LI Silver enhancement was performed for 5 min. After rinsing with H₂O, specimens were embedded in Epon 812, and sections were cut orthogonally to the cell monolayer with a diamond knife. Thin sections were visualized in a JEOL 1010 TEM.

Oleinik N., Kim J., Roth B.M., Selvam S.P., Gooz M., Johnson R.H., Lemasters J.J, & Ogretmen B. (2019). Mitochondrial protein import is regulated by p17/PERMIT to mediate lipid metabolism and cellular stress. Science Advances, 5(9), eaax1978.

+ Open protocol

+ Expand

Phyto-synthesized Selenium Nanoparticles: Morphological Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

The morphological characteristics such as size, shape, and agglomeration of Phyto-synthesized Se-NPs were investigated using TEM (TEM JEOL 1010, Tokyo, Japan). Briefly, 1 g of synthesized Se-NPs was dissolved in 2 mL ethanol and sonicated for 10 min. After that, a few drops of suspension were loaded on a Cu-grid and stand-up to complete adsorption. The loaded Cu-grid was dabbed on blotting paper to remove excess solution before being subjected to image capture [24 (link)].

Fouda A., Al-Otaibi W.A., Saber T., AlMotwaa S.M., Alshallash K.S., Elhady M., Badr N.F, & Abdel-Rahman M.A. (2022). Antimicrobial, Antiviral, and In-Vitro Cytotoxicity and Mosquitocidal Activities of Portulaca oleracea-Based Green Synthesis of Selenium Nanoparticles. Journal of Functional Biomaterials, 13(3), 157.

+ Open protocol

+ Expand

Antibody Segregation via Optimized Conjugation

Check if the same lab product or an alternative is used in the 5 most similar protocols

With the aim to directly observe if the optimized conjugation method leads to the segregation of a mix of antibodies in two different domains (polarization), we performed a straight forward experiment. On the one hand, AuNPs of ~60 nm were doubly conjugated with rabbit anti-HSA IgG (18 μg/ml) and rabbit anti-BSA IgG (18 μg/ml). On the other hand, we conjugated smaller AuNPs as labels: AuNPs of 25 nm were functionalized with BSA (5 μM) and of 15 nm functionalized with HSA (5 μM). The bispecific conjugates were incubated with the AuNPs functionalized with BSA and the AuNPs functionalized with HSA for 24 h. Then, the sample containing the three types of AuNPs was purified from the excess of smaller AuNPs by decantation and after that, the TEM grids were prepared by dipping, by directly immersing the grids in the AuNPs solution and letting them dry in an open atmosphere overnight. Finally, the sample was analyzed by TEM, using an 80-keV TEM JEOL 1010 equipped with an Orius (Gatan) CCD Camera. TEM grids consisted on an ultrathin-formvar coated 200 mesh copper grid covered with a layer of carbon (Ted-Pella). The TEM images of 232 AuNPs of 60 nm were randomly obtained and classified depending on the apparent polarization formed by the specific recognition between 25 nm and 15 nm label AuNPs.

Astorga-Gamaza A., Vitali M., Borrajo M.L., Suárez-López R., Jaime C., Bastus N., Serra-Peinado C., Luque-Ballesteros L., Blanch-Lombarte O., Prado J.G., Lorente J., Pumarola F., Pellicer M., Falcó V., Genescà M., Puntes V, & Buzon M.J. (2020). Antibody cooperative adsorption onto AuNPs and its exploitation to force natural killer cells to kill HIV-infected T cells. Nano today, 36, 101056.

+ Open protocol

+ Expand

Micelle Characterization by DLS and TEM

Check if the same lab product or an alternative is used in the 5 most similar protocols

Micelles of 1 were prepared via thin film hydration followed by sonication. Bath sonication (Branson 2510 Ultrasonic Cleaner, Branson Ultrasonics, Danbury, CT, USA) was carried out at 50 °C for 5 min. Particle size distributions were determined using a Malvern Zetasizer Nano ZS (Malvern Instruments Ltd., Malvern, UK) equipped with a peltier controlled thermostatic holder, a fixed wavelength at 633 nm and scattering angle of 173°. DLS measurements were carried out at room temperature. For TEM observation, a drop of 1 (300 μM) was placed onto a nitrocellulose membrane covered TEM copper grid and dabbed dry through the underside of the grid with a tissue. This was then washed three times with ddH₂O. A drop of uranyl acetate (2% w/v) in H₂O was then added and the sample left to dry in the dark. Transmission electron microscopy (TEM JEOL 1010; JEOL Ltd., Tokyo, Japan; Nieuw-Vennep, The Netherlands) was run at an accelerating voltage of 60 kV.

Kong L., Poulcharidis D., Schneider G.F., Campbell F, & Kros A. (2017). Spatiotemporal Control of Doxorubicin Delivery from “Stealth-Like” Prodrug Micelles. International Journal of Molecular Sciences, 18(10), 2033.

+ Open protocol

+ Expand

Characterization of Bacterially Synthesized ZnO-NPs

Check if the same lab product or an alternative is used in the 5 most similar protocols

The morphological characteristics of bacterially synthesized ZnO-NPs were investigated by TEM analysis (TEM-JEOL 1010, Tokyo, Japan). A few drops of the bacterially synthesized ZnO-NPs suspension were loaded onto the carbon–copper TEM grid. The excess ZnO-NPs’ solution on the TEM grid was removed by contacting the grid with blotting paper. The loaded TEM grid was left to dry at room temperature before being subjected to analysis [28 (link)].

Abdo A.M., Fouda A., Eid A.M., Fahmy N.M., Elsayed A.M., Khalil A.M., Alzahrani O.M., Ahmed A.F, & Soliman A.M. (2021). Green Synthesis of Zinc Oxide Nanoparticles (ZnO-NPs) by Pseudomonas aeruginosa and Their Activity against Pathogenic Microbes and Common House Mosquito, Culex pipiens. Materials, 14(22), 6983.

+ Open protocol

+ Expand

Negative Staining Techniques for TEM

Check if the same lab product or an alternative is used in the 5 most similar protocols

Samples were adsorbed to the glow discharged carbon film coated 300 mesh copper grids, washed with water, stained, and air-dried. Samples were stained with 2% uranyl acetate (Figure 1) or 6% ammonium molybdate (6%) and trehalose (0.5%) (Figure 2). Trehalose is added to minimize the collapse of particles that occurs on the grid upon dehydration. Images were collected with a JEOL TEM 1010 (Figure 1) or a JEOL 1400plus microscope (Figure 2).

Schlicksup C.J., Laughlin P., Dunkelbarger S., Wang J.C, & Zlotnick A. (2020). Local Stabilization of Subunit–Subunit Contacts Causes Global Destabilization of Hepatitis B Virus Capsids. ACS chemical biology, 15(6), 1708-1717.

+ Open protocol

+ Expand

Negative Staining of Nanoparticles for TEM

Check if the same lab product or an alternative is used in the 5 most similar protocols

Nanoparticles were negatively stained with 1.5% uranyl acetate, and images were taken using a JEOL TEM-1010 transmission electron microscope (JEOL USA, Inc., Peabody, MA) operating at an acceleration voltage of 80 kV. The images were captured at a 20,000× magnification.

Mussi S.V., Parekh G., Pattekari P., Levchenko T., Lvov Y., Ferreira L.A, & Torchilin V.P. (2015). Improved pharmacokinetics and enhanced tumor growth inhibition using a nanostructured lipid carrier loaded with doxorubicin and modified with a layer-by-layer polyelectrolyte coating. International journal of pharmaceutics, 495(1), 186-193.

+ Open protocol

+ Expand

Transmission Electron Microscopy of EVs

Check if the same lab product or an alternative is used in the 5 most similar protocols

TEM analysis was performed as described previously, with minor modifications [6 (link)]. Briefly, the concentrated EV fractions, prepared as described above, were mixed with 25% glutaraldehyde in phosphate buffer (pH 7.2) in a ratio of 9:1 and then applied to collodion-coated 200-mesh copper grids. The grids were stained with 2% uranyl acetate (pH 7) and embedded with 2% methylcellulose/0.4% uranyl acetate (pH 4). After drying, the grids were examined using TEM (TEM-1010; JEOL, Tokyo, Japan).

Ogawa Y., Akimoto Y., Ikemoto M., Goto Y., Ishikawa A., Ohta S., Takase Y., Kawakami H., Tsujimoto M, & Yanoshita R. (2021). Stability of human salivary extracellular vesicles containing dipeptidyl peptidase IV under simulated gastrointestinal tract conditions. Biochemistry and Biophysics Reports, 27, 101034.

+ Open protocol

+ Expand

Comprehensive Characterization of MBAuNPs

Check if the same lab product or an alternative is used in the 5 most similar protocols

The synthesized MBAuNPs were characterized using a SpectraMax Paradigm to obtain the absorption spectrum of these particles and a JOEL transmission electron microscope (TEM) 1010 was used to obtain the size and shape of the nanoparticles. For this, 10 μL of the washed MBAuNP was placed on a neat parafilm and the copper grid was placed on the droplet and dried to remove excess solution. Then, they were scanned using a JEOL TEM 1010. A Hitachi 4800 Scanning Electron Microscope was used to obtain the morphology of the nanoparticles. For this, a sample holder was coated with a palladium coating and the MBAuNPs were placed on the coated sample holder and dried; then, it was scanned in the scanning electron microscope. Lastly, a DLS 90 Plus Brookhaven instrument was used to obtain the hydrodynamic size of the particles.

Johnston J., Taylor E.N., Gilbert R.J, & Webster T.J. (2015). Improved molecular fingerprint analysis employing multi-branched gold nanoparticles in conjunction with surface-enhanced Raman scattering. International Journal of Nanomedicine, 11, 45-53.

+ Open protocol

+ Expand

Transmission Electron Microscopy Analysis of Extracellular Vesicles

Check if the same lab product or an alternative is used in the 5 most similar protocols

Transmission electron microscopy (TEM) analysis was performed in accordance with a previously described method (Ogawa et al., 2011 (link)), with a few modifications. In brief, the EV fractions, prepared as described earlier, were mixed with 25% glutaraldehyde (G011/1, TAAB Laboratories Equipment Ltd., Aldermaston, UK) in phosphate buffer (pH 7.2) at a ratio of 9:1. The 2.5 µL of samples were then applied onto collodion-coated 200-mesh copper grids (collodion support film on 200 mesh copper grids, No. 6511, Nisshin EM Corporation, Tokyo, Japan). After standing the samples for 5 s, the grids were then stained with 2% uranyl acetate (pH 7.0) for 5 s. Excess uranyl acetate solution on the grids was absorbed with filter paper and embedded in 2% methylcellulose/0.4% uranyl acetate (pH 4.0). The grids were then dried and examined using TEM (TEM-1010; JEOL, Tokyo, Japan) at 80 kV.

Ogawa Y., Miura Y., Ikemoto M., Ohnishi A., Goto Y., Aoki K., Motokurumada Y., Akimoto Y., Endo T., Tsujimoto M, & Yanoshita R. (2024). Distinguishing two distinct types of salivary extracellular vesicles: a potential tool for understanding their pathophysiological roles. Frontiers in Molecular Biosciences, 11, 1278955.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!