Log In Sign up
The largest database of trusted experimental protocols

Sta 320

Manufactured by Cell Biolabs
Visit Supplier
Sourced in United States

The STA-320 is a laboratory instrument designed for the detection and quantification of cellular senescence. It utilizes a specialized staining method to measure the activity of senescence-associated β-galactosidase, a key marker of cellular senescence. The STA-320 provides accurate and reliable data on the senescent status of cell samples, enabling researchers to study age-related cellular changes and cellular aging processes.

Automatically generated - may contain errors

Lab products found in correlation

C11 bodipy probe, by Thermo Fisher Scientific (1 mentions) Ab83366, by Abcam (1 mentions) Qiamp dna isolation kit, by Qiagen (1 mentions) Allprep dna rna protein mini, by Qiagen (1 mentions) Nuclease p1, by Merck Group (1 mentions) Alkaline phosphatase, by Merck Group (1 mentions) Csb e14362m, by Cusabio (1 mentions)

6 protocols using sta 320

1

Quantifying Biomarkers in Biological Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
Commercially available enzyme-linked immunosorbant assay (ELISA) kits were used to measure the concentrations or activity of HMGB1 (Shino Test Corporation, ST51011), amylase (BioVision, K711), MPO (BioVision, K744), iron (Abcam, ab83366), MDA (Abcam, ab118970), and 8-OHdG (Cell Biolabs, STA-320) in indicated samples according to the manufacturer’s instructions. Data were normalized to protein or DNA concentration. In addition, C11-BODIPY probe (Thermo Fisher Scientific, D3861) was used to detect lipid ROS in cells.
Liu J., Song X., Kuang F., Zhang Q., Xie Y., Kang R., Kroemer G, & Tang D. (2021). NUPR1 is a critical repressor of ferroptosis. Nature Communications, 12, 647.
+ Open protocol
+ Expand
2

Quantification of 8-oxodG Levels in Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
A commercially available ELISA kit from Cell Biolabs (STA-320) was used to measure levels of 8-oxodG. Although this 8-oxodG ELISA has potential shortcomings for the precise quantification of 8-oxodG levels, it allows an estimation of the change of 8-oxodG levels upon triamterene treatment. Genomic DNA was extracted using the QIamp DNA isolation kit (Qiagen), digested with nuclease P1, treated with calf intestinal phosphatase and denatured. To avoid artifactual production of 8-oxodG, we used a phenol-free method of DNA isolation and DNA was completely digested. The assays were performed according to the manufacturer's instructions. Briefly, 10-15 µg DNA from untreated and treated cells or the 8-oxodG standard (0.078–20 ng/ml) was incubated with an 8-oxodG monoclonal antibody in an 8-oxodG-precoated microtiter plate. The assay was normalized by using an equal amount of DNA for each sample. Standard curves were calculated with serial dilutions of 8-oxodG standard to calculate reaction efficiency. Samples were assayed in triplicate.
Guillotin D., Austin P., Begum R., Freitas M.O., Merve A., Brend T., Short S., Marino S, & Martin S.A. (2016). Drug-repositioning screens identify Triamterene as a selective drug for the treatment of DNA Mismatch Repair deficient cells. Clinical cancer research : an official journal of the American Association for Cancer Research, 23(11), 2880-2890.
+ Open protocol
+ Expand
3

Urinary 8-oxo-dGuo Quantification by ELISA

Check if the same lab product or an alternative is used in the 5 most similar protocols
Urinary 8-oxo-dGuo level was quantified using a competitive enzyme immunoassay (STA-320, Cell Biolabs, San Diego, CA). Briefly, urine samples (1 : 20 dilution) or 8-oxo-dGuo standards were first added to an 8-oxo-dGuo/BSA conjugate preabsorbed enzyme immunoassay plate. After incubation, an anti-8-oxo-dGuo monoclonal antibody was added, followed by a secondary reaction with a horseradish peroxidase-conjugated antibody. 8-oxo-dGuo levels in the urine samples were then determined by comparison with the 8-oxo-dGuo standard curve. 8-oxo-dGuo levels in the urine of each subject were adjusted by urinary creatinine level and were measured as ng/mg creatinine.
Chaisiriwong L., Wanitphakdeedecha R., Sitthinamsuwan P., Sampattavanich S., Chatsiricharoenkul S., Manuskiatti W, & Panich U. (2016). A Case-Control Study of Involvement of Oxidative DNA Damage and Alteration of Antioxidant Defense System in Patients with Basal Cell Carcinoma: Modulation by Tumor Removal. Oxidative Medicine and Cellular Longevity, 2016, 5934024.
+ Open protocol
+ Expand
4

Quantifying Oxidative Stress Biomarker 8-OHdG

Check if the same lab product or an alternative is used in the 5 most similar protocols
Production of 8-OHdG in serum was determined by ELISA (STA-320; Cell Biolabs, San Diego, CA, USA) as described by Weaver et al [48 (link)]. Undiluted samples were added to an 8-OHdG conjugate-coated microplate, followed by diluted anti-8-OHdG antibody, and finally diluted secondary antibody enzyme conjugate. After incubation, the provided stop solution was added to each well, and allowed to incubate for 8–10 min before being stopped with a stop solution in order to achieve a color change which was not over-saturated. Samples were then measured at 450 nm and concentration was determined based on the standard curve.
Kim S.W., Holanda D.M., Gao X., Park I, & Yiannikouris A. (2019). Efficacy of a Yeast Cell Wall Extract to Mitigate the Effect of Naturally Co-Occurring Mycotoxins Contaminating Feed Ingredients Fed to Young Pigs: Impact on Gut Health, Microbiome, and Growth. Toxins, 11(11), 633.
+ Open protocol
+ Expand
5

Quantifying 8-OHdG in Liver DNA

Check if the same lab product or an alternative is used in the 5 most similar protocols
Genomic DNA was extracted from 50 mg frozen liver tissue using a purification spin column followed by DNA elution according to manufacturer's instructions (Qiagen AllPrep DNA/RNA/Protein Mini). Extracted DNA was converted to single-stranded DNA by incubation at 95 °C for 5 min then rapidly chilled on ice. Single-stranded DNA was then digested to nucleosides in 20 mM sodium acetate buffer pH 5.2 containing nuclease P1 (Sigma N8630) for 2 h, followed by incubation in 100 mM Tris buffer containing alkaline phosphatase (Sigma P6774) at a final pH of 7.5 at 37 °C for 1 h. The nucleoside-containing mixture was centrifuged at 6,000 g for 5 min and the supernatant was used for the 8-OHdG assay. 8-OHdG was quantitated using an enzyme-linked immunosorbent assay according to the manufacturer's protocol (Cell Biolabs STA-320).
Nelson M.E., Lahiri S., Chow J.D., Byrne F.L., Hargett S.R., Breen D.S., Olzomer E.M., Wu L.E., Cooney G.J., Turner N., James D.E., Slack-Davis J.K., Lackner C., Caldwell S.H, & Hoehn K.L. (2017). Inhibition of hepatic lipogenesis enhances liver tumorigenesis by increasing antioxidant defence and promoting cell survival. Nature Communications, 8, 14689.
+ Open protocol
+ Expand
6

Serum Klotho and 8-OHdG Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Enzyme-linked immunosorbent assay (ELISA) kits were used to detect Klotho (CSB-E14362m; Cusabio Technology, Houston, TX, USA) and 8-OHdG (STA-320; Cell Biolabs, San Diego, CA, USA) in serum, according to the manufacturer’s instruction.
Lim S.W., Shin Y.J., Luo K., Quan Y., Cui S., Ko E.J., Chung B.H, & Yang C.W. (2019). Ginseng increases Klotho expression by FoxO3-mediated manganese superoxide dismutase in a mouse model of tacrolimus-induced renal injury. Aging (Albany NY), 11(15), 5548-5569.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!