Nickel chloride hexahydrate (NiCl2·6H2O), cobalt chloride hexahydrate (CoCl2·6H2O), NaOH, and HCl were purchased from Shanghai Macklin Biochemical Co. (Shanghai, China). Tris-HCl Buffer (1M, pH8.8) was ordered from Aladdin Reagent Co. Macroporous ion exchange resins were obtained from Jiangsu Suqing Water Treatment Engineering Group Co., Ltd. (Jiangyin, China).
Recombinant strain GLpmi-QCDC was obtained from Dr. Zhongyang Ding. Key gene of gl-pmi was cloned from G. lucidum strain CGMCC 5.26 and expressed in E. coli. BL21 (DE3). The recombinant strains GLpmi-QCDC was induced and expressed in E. coli [32 ]. LB medium was prepared using the following recipe: tryptone 10.0 (g·L−1), yeast extract 5.0 (g·L−1), and NaCl 10.0 (g·L−1). 1 × PBS buffer included: KH2PO4 0.27 (g·L−1), Na2HPO4 1.42 (g·L−1), NaCl 8.00 (g·L−1), and KCl 0.20 (g·L−1); the pH was adjusted to pH 7.4 using HCl.
Recombinant strain GLpmi-QCDC was obtained from Dr. Zhongyang Ding. Key gene of gl-pmi was cloned from G. lucidum strain CGMCC 5.26 and expressed in E. coli. BL21 (DE3). The recombinant strains GLpmi-QCDC was induced and expressed in E. coli [32 ]. LB medium was prepared using the following recipe: tryptone 10.0 (g·L−1), yeast extract 5.0 (g·L−1), and NaCl 10.0 (g·L−1). 1 × PBS buffer included: KH2PO4 0.27 (g·L−1), Na2HPO4 1.42 (g·L−1), NaCl 8.00 (g·L−1), and KCl 0.20 (g·L−1); the pH was adjusted to pH 7.4 using HCl.